APRIL (S183) polyclonal antibody 
Datasheet
COA
MSDS
SHIP
Product Name :
APRIL (S183) polyclonal antibody Background :
A proliferation-inducing ligand (APRIL), also designated TNFSF13, is a Type II membrane protein that shares characteristics with other members of the tumor necrosis factor (TNF) cytokine family. APRIL is expressed in high levels in transformed cell lines and in human colon, thyroid, and lymphoid tumor tissues. APRIL is critically involved in the regulation of infections, inflammation, autoimmune diseases, and tissue homeostasis. APRIL is implicated in the regulation of tumor cell growth. The C-terminal extracellular domain has b jelly roll topography and is important in ligand trimerization. The binding of the ligand to its respective receptor induces oligomerization, initiating downstream signaling events. Intrinsic to oligomerization is the formation of the receptor binding site at the junction between neighboring subunits, creating a multivalent ligand. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
APRIL (S183) polyclonal antibody detects endogenous levels of APRIL protein. Immunogen :
Synthetic peptide, corresponding to amino acids 151-200 of Human APRIL. Conjugate :
Unconjugated Modification :
Unmodification
APRIL (S183) polyclonal antibody Background :
A proliferation-inducing ligand (APRIL), also designated TNFSF13, is a Type II membrane protein that shares characteristics with other members of the tumor necrosis factor (TNF) cytokine family. APRIL is expressed in high levels in transformed cell lines and in human colon, thyroid, and lymphoid tumor tissues. APRIL is critically involved in the regulation of infections, inflammation, autoimmune diseases, and tissue homeostasis. APRIL is implicated in the regulation of tumor cell growth. The C-terminal extracellular domain has b jelly roll topography and is important in ligand trimerization. The binding of the ligand to its respective receptor induces oligomerization, initiating downstream signaling events. Intrinsic to oligomerization is the formation of the receptor binding site at the junction between neighboring subunits, creating a multivalent ligand. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
APRIL (S183) polyclonal antibody detects endogenous levels of APRIL protein. Immunogen :
Synthetic peptide, corresponding to amino acids 151-200 of Human APRIL. Conjugate :
Unconjugated Modification :
Unmodification
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Western blot (WB) analysis of APRIL (S183) pAb at 1:500 dilution Lane1:SGC7901 whole cell lysate(40ug) Lane2:A549 whole cell lysate(40ug) Lane3:Panc1 whole cell lysate(40ug) Lane4:H9C2 whole cell lysate(40ug) Lane5:CT26 whole cell lysate(40ug) -
Immunohistochemistry (IHC) analyzes of APRIL (S183) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.
Amelioration of Colorectal Cancer Using Negative Lipidoid Nanoparticles to Encapsulate siRNA Against APRIL by Enema Delivery Mode
PMCID: Pubmed No.:24771268
Amelioration of Colorectal Cancer Using Negative Lipidoid Nanoparticles to Encapsulate siRNA Against APRIL by Enema Delivery Mode
PMCID: Pubmed No.:24771268
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.
Price/Size :
USD 368/1mg/vial
Tips:
For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).Describe :
Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.Formula:
Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.
Storage:
The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C.
Note :
This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.