Product Name :
BCKDK (E41) polyclonal antibody Background :
BCKDK (branched chain ketoacid dehydrogenase kinase), also known as BCKDHKIN, is a 412 amino acid mitochondrial matrix protein that exists as a monomer and contains one histidine kinase domain. Expressed ubiquitously, BCKDK catalyzes the ATP-dependent phosphorylation and subsequent inactivation of the branched-chain α-ketoacid dehydrogenase (BCKD) complex, a regulatory enzyme complex that plays a crucial role in the catabolic pathways of valine, leucine and isoleucine. Specifically, the BCKD complex functions as the second enzyme in branched-chain amino acid (BCAA) catabolism, effectively catalyzing the irreversible oxidative decarboxylation of BCAAs. Due to the ability of BCKDK to regulate the activity of the BCKD complex, BCKDK plays an essential role in the catabolic pathways of branched-chain amino acid metabolism. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
BCKDK (E41) polyclonal antibody detects endogenous levels of BCKDK protein. Immunogen :
Synthetic peptide, corresponding to amino acids 21-70 of Human BCKDK. Conjugate :
Unconjugated Modification :
Unmodification

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  Western blot (WB) analysis of BCKDK (E41) polyclonal antibody at 1:500 dilution Lane1:C6 whole cell lysate(40ug) Lane2:AML-12 whole cell lysate(40ug) Lane3:HEK293T whole cell lysate(40ug) Lane4:L02 whole cell lysate(40ug)
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  Immunohistochemistry (IHC) analyzes of BCKDK (E41) pAb in paraffin-embedded human tonsil carcinoma tissue at 1:50.
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  Immunohistochemistry (IHC) analyzes of BCKDK (E41) pAb in paraffin-embedded human tonsil carcinoma tissue at 1:50.

Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

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Price/Size :

USD 368/1mg/vial

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Tips: 

For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).

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Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

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Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

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Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 

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Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.

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