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AKT (A444) polyclonal antibody BS1810
  • Western blot (WB) analysis of AKT (A444) polyclonal antibody at 1:500 dilution Lane1:MCF-7 whole cell lysate(40ug) Lane2:The Lung tissue lysate of Rat(40ug) Lane3:The Lung tissue lysate of Mouse(40ug)
  • Immunohistochemistry (IHC) analyzes of AKT (A444) pAb in paraffin-embedded human breast carcinoma tissue at 1:50,showing cytoplasm and nucleus staining.Negative control (the right)Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.
  • Immunohistochemistry (IHC) analyzes of AKT (A444) pAb in paraffin-embedded human breast carcinoma tissue at 1:50,showing cytoplasm and nucleus staining.Negative control (the right)Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.
Product NameAKT (A444) polyclonal antibody
Catalog No.BS1810
Swiss-ProtP31749/P31751/Q9Y243
Host Rabbit
ReactivityHuman,Mouse,Rat
ApplicationsWB IHC IP
Application_allWB 1:500 - 1:1000 IHC 1:50 - 1:100 IP 1:10 - 1:100
BiowMW~ 60 kDa
Alternative NameRAC-alpha serine/threonine-protein kinase; Protein kinase B; PKB; Protein kinase B alpha; PKB alpha; Proto-oncogene c-Akt; RAC-PK-alpha; AKT1; PKB; RAC; PKBα; RAC-beta serine/threonine-protein kinase; Protein kinase Akt-2; Protein kinase B beta; PKB beta; RAC-PK-beta; AKT2; PKBβ; RAC-gamma serine/threonine-protein kinase; Protein kinase Akt-3; Protein kinase B gamma; PKB gamma; RAC-PK-gamma; STK-2; AKT3; PKBG; PKBγ
Purification&PurityThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
ConjugateUnconjugated
ModificationUnmodification
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100ul $275
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Product Name :
AKT (A444) polyclonal antibody
Background :
AKT, also known as protein kinase B (PKB), is a 57 kDa serine/threonine protein kinase. There are three mammalian isoforms of Akt: AKT1 (PKB alpha), AKT2 (PKB beta) and AKT3 (PKB gamma) with AKT2 and AKT3 being approximately 82% identical with the AKT1 isoform. Each isoform has a pleckstrin homology (PH) domain, a kinase domain and a carboxy terminal regulatory domain. AKT was originally cloned from the retrovirus AKT8, and is a key regulator of many signal transduction pathways. Its tight control over cell proliferation and cell viability are manifold; overexpression or inappropriate activation of AKT has been seen in many types of cancer.
Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity :
AKT (A444) polyclonal antibody detects endogenous levels of total AKT protein.
Immunogen :
Synthetic peptide, corresponding to amino acids 420-470 of Human AKT1.
Conjugate :
Unconjugated
Modification :
Unmodification
  • Western blot (WB) analysis of AKT (A444) polyclonal antibody at 1:500 dilution Lane1:MCF-7 whole cell lysate(40ug) Lane2:The Lung tissue lysate of Rat(40ug) Lane3:The Lung tissue lysate of Mouse(40ug)
  • Immunohistochemistry (IHC) analyzes of AKT (A444) pAb in paraffin-embedded human breast carcinoma tissue at 1:50,showing cytoplasm and nucleus staining.Negative control (the right)Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.
  • Immunohistochemistry (IHC) analyzes of AKT (A444) pAb in paraffin-embedded human breast carcinoma tissue at 1:50,showing cytoplasm and nucleus staining.Negative control (the right)Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.
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PMCID:    Pubmed No.:24098937 Musashi2 modulates K562 leukemic cell proliferation and apoptosis involving the MAPK pathway PMCID:    Pubmed No.:24076374 Quercetin oppositely regulates insulin‐mediated glucose disposal in skeletal muscle under normal and inflammatory conditions: The dual roles of AMPK activation PMCID:    Pubmed No.:26627467 Ribonuclease inhibitor up-regulation inhibits the growth and induces apoptosis in murine melanoma cells through repression of angiogenin and ILK/PI3K/AKT signaling pathway. PMCID:    Pubmed No.:24769129 Tripterygium Glycosides Impairs the Proliferation of Granulosa Cells and Decreases the Reproductive Outcomes in Female Rats. PMCID:    Pubmed No.:24831781 Quercetin differently regulates insulin-mediated glucose transporter 4 translocation under basal and inflammatory conditions in adipocytes PMCID:    Pubmed No.:24343960 The DPP-4 inhibitor sitagliptin attenuates the progress of atherosclerosis in apolipoprotein-E-knockout mice via AMPK-and MAPK-dependent mechanisms PMCID:    Pubmed No.:24490809 Cyclocarya paliurus extract modulates adipokine expression and improves insulin sensitivity by inhibition of inflammation in mice PMCID:    Pubmed No.:24530856 cyclooxygenase-2 inhibitor is a robust enhancer of anticancer agents against hepatocellular carcinoma multicellular spheroids PMCID:    Pubmed No.:24591842 Akt1 signaling coordinates BMP signaling and β-catenin activity to regulate second heart field progenitor development PMCID:    Pubmed No.:25670795 Ginsenoside Rb1 and compound K improve insulin signaling and inhibit ER stress-associated NLRP3 inflammasome activation in adipose tissue PMCID:    Pubmed No.:27746687 Mangiferin suppresses endoplasmic reticulum stress in perivascular adipose tissue and prevents insulin resistance in the endothelium PMCID:    Pubmed No.:30666402 Ginsenoside Rg5 Inhibits Succinate-Associated Lipolysis in Adipose Tissue and Prevents Muscle Insulin Resistance PMCID:    Pubmed No.:28261091 Establishment of human pancreatic cancer gemcitabine‑resistant cell line with ribonucleotide reductase overexpression PMCID:    Pubmed No.:25394408 Metformin and resveratrol ameliorate muscle insulin resistance through preventing lipolysis and inflammation in hypoxic adipose tissue PMCID:    Pubmed No.:27343375 Estrogen-related receptor (ERR) γ protects against puromycin aminonucleoside-induced podocyte apoptosis by targeting PI3K/Akt signaling PMCID:    Pubmed No.:27417234 Pretreatment with low dose etomidate prevents etomidate-induced rat adrenal insufficiency by regulating oxidative stress-related MAPKs and apoptosis PMCID:    Pubmed No.:25989534 Modified Si-Miao-San inhibits inflammation and promotes glucose disposal in adipocytes through regulation of AMP-kinase PMCID:    Pubmed No.:25556062 Mangiferin ameliorates insulin resistance by inhibiting inflammation and regulatiing adipokine expression in adipocytes under hypoxic condition PMCID:    Pubmed No.:28991527 Clostridium butyricum exerts a neuroprotective effect in a mouse model of traumatic brain injury via the gut‐brain axis PMCID:    Pubmed No.:29193450 Downregulation of prostaglandin E2 is involved in hindgut mucosal damage in lactating goats fed a high‐concentrate diet PMCID:    Pubmed No.:26593381 CXCR4 knockdown inhibits the growth and invasion of nasopharyngeal cancer stem cells PMCID:    Pubmed No.:28454388 Vitamin C sensitizes BRAFV600E thyroid cancer to PLX4032 via inhibiting the feedback activation of MAPK/ERK signal by PLX4032 PMCID:    Pubmed No.:33468157 Rare ginsenosides ameliorate lipid overload-induced myocardial insulin resistance via modulating metabolic flexibility PMCID:    Pubmed No.:31005715 Lnc-MCEI mediated the chemosensitivity of esophageal squamous cell carcinoma via miR-6759-5p to competitively regulate IGF2 PMCID:    Pubmed No.:33061807 ERα and/or ERβ activation ameliorates cognitive impairment, neurogenesis and apoptosis in type 2 diabetes mellitus mice PMCID:    Pubmed No.:30201537 Neuroprotective effects of novel compound Tozan on cognition, neurogenesis and apoptosis in diabetes PMCID:    Pubmed No.:34734023 EHF enhances malignancy by modulating AKT and MAPK/ERK signaling in non‑small cell lung cancer cells PMCID:    Pubmed No.:33907840 Activation of ERα and/or ERβ ameliorates cognitive impairment and apoptosis in streptozotocin-induced diabetic mice PMCID:    Pubmed No.:30096284 CXCR3 expression in colorectal cancer cells enhanced invasion through preventing CXCR4 internalization PMCID:    Pubmed No.:30092218 Gas6 attenuates lipopolysaccharide‑induced TNF‑α expression and apoptosis in H9C2 cells through NF‑κB and MAPK inhibition via the Axl/PI3K/Akt pathway PMCID:    Pubmed No.:31524235 Galectin‑3 facilitates the proliferation and migration of nasopharyngeal carcinoma cells via activation of the ERK1/2 and Akt signaling pathways, and is positively correlated with the inflammatory state of nasopharyngeal carcinoma PMCID:    Pubmed No.:33760180 ROS induces epithelial‑mesenchymal transition via the TGF‑β1/PI3K/Akt/mTOR pathway in diabetic nephropathy PMCID:    Pubmed No.:30651870 Synthesis of dihydrofuran-3-one and 9,10-phenanthrenequinone hybrid molecules and biological evaluation against colon cancer cells as selective Akt kinase inhibitors PMCID:    Pubmed No.:35751771 Mechanism of Shiliu Buxue Syrup for anemia using integrated metabolomics and network pharmacology PMCID:    Pubmed No.:35690102 Neuroprotective effects of novel compound FMDB on cognition, neurogenesis and apoptosis in APP/PS1 transgenic mouse model of Alzheimer's disease PMCID:    Pubmed No.:36893915
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

Price/Size :

USD 368/1mg/vial



Tips: 

For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).

Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 


Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.
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