Wnt-1 (G331) polyclonal antibody 
Datasheet
COA
MSDS
SHIP
Product Name :
Wnt-1 (G331) polyclonal antibody Background :
Products of the highly conserved Wnt gene family play key roles in regulating cellular growth and differentiation. The prototype member of the Wnt gene family, Wnt-1, is a cysteine-rich secreted glycoprotein that associates with cell membranes and likely functions as a key regulator of cellular adhesion. β-catenin, a cadherin-binding cellular adhesion protein which also binds the tumor supressor gene APC, has been identified as a downstream target of a signal transduction pathway mediated by Wnt-1. Wnt-1 is essential for normal development of the embryonic nervous system and its expression is normally limited to the embryonic neural tube and adult spermatids. When improperly expressed in mammary tissue, Wnt-1 contributes to hyperplasia and tumorigenic progression. Wnt family members have been shown to interact with Sonic hedgehog (Shh) in vivo to induce myogenesis in somitic tissue. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
Wnt-1 (G331) polyclonal antibody detects endogenous levels of Wnt-1 protein. Immunogen :
Synthetic peptide, corresponding to amino acids 301-350 of Human Wnt-1. Conjugate :
Unconjugated Modification :
Unmodification
Wnt-1 (G331) polyclonal antibody Background :
Products of the highly conserved Wnt gene family play key roles in regulating cellular growth and differentiation. The prototype member of the Wnt gene family, Wnt-1, is a cysteine-rich secreted glycoprotein that associates with cell membranes and likely functions as a key regulator of cellular adhesion. β-catenin, a cadherin-binding cellular adhesion protein which also binds the tumor supressor gene APC, has been identified as a downstream target of a signal transduction pathway mediated by Wnt-1. Wnt-1 is essential for normal development of the embryonic nervous system and its expression is normally limited to the embryonic neural tube and adult spermatids. When improperly expressed in mammary tissue, Wnt-1 contributes to hyperplasia and tumorigenic progression. Wnt family members have been shown to interact with Sonic hedgehog (Shh) in vivo to induce myogenesis in somitic tissue. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
Wnt-1 (G331) polyclonal antibody detects endogenous levels of Wnt-1 protein. Immunogen :
Synthetic peptide, corresponding to amino acids 301-350 of Human Wnt-1. Conjugate :
Unconjugated Modification :
Unmodification
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Western blot (WB) analysis of Wnt-1 (G331) polyclonal antibody at 1:500 dilution Lane1:HEK293T whole cell lysate(40ug) Lane2:Hela whole cell lysate(40ug) Lane3:DLD whole cell lysate(40ug) Lane4:The Testis tissue lysate of Mouse(40ug) Lane5:The Testis tissue lysate of Rat(40ug) -
Immunohistochemistry (IHC) analyzes of Wnt-1 (G331) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.
Role of Wnt1 and Fzd1 in the spinal cord pathogenesis of amyotrophic lateral sclerosis-transgenic mice
PMCID: Pubmed No.:23553522
Sox9 regulates hyperexpression of Wnt1 and Fzd1 in human osteosarcoma tissues and cells
PMCID: Pubmed No.:25197350
Sox9 regulates hyperexpression of Wnt1 and Fzd1 in human osteosarcoma tissues and cells
PMCID: Pubmed No.:25197350
Wnt and Notch signaling pathway involved in wound healing by targeting c-Myc and Hes1 separately
PMCID: Pubmed No.:26076648
Endothelial‑to‑mesenchymal transition in human idiopathic dilated cardiomyopathy
PMCID: Pubmed No.:29115553
DNMT1-Induced miR-152-3p Suppression Facilitates Cardiac Fibroblast Activation in Cardiac Fibrosis
PMCID: Pubmed No.:34424481
Umbelliferone Inhibits Migration, Invasion and Inflammation of Rheumatoid Arthritis Fibroblast-Like Synoviocytes and Relieves Adjuvant-Induced Arthritis in Rats by Blockade of Wnt/β-Catenin Signaling Pathway
PMCID: Pubmed No.:35997647
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.
Price/Size :
USD 368/1mg/vial
Tips:
For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).Describe :
Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.Formula:
Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.
Storage:
The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C.
Note :
This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.