Survivin (N111) polyclonal antibody 
Datasheet
COA
MSDS
SHIP
Product Name :
Survivin (N111) polyclonal antibody Background :
Two mammalian homologs of baculovirus p35, referred to as inhibitor of apoptosis protein (IAP) 1 and 2, share an amino-terminal baculovirus IAP repeat (BIR) motif and a carboxy-terminal RING finger. Although the c-IAPs do not directly associate with the TNF receptor (TNF-R), they efficiently block TNF-mediated apoptosis through their interaction with the downstream TNF-R effectors, TRAF1 and TRAF2. Additional IAP family members include ILP (for IAP-like protein) and survivin. ILP inhibits activated caspase-3, leading to the resistance of FASmediated apoptosis. Survivin (also designated TIAP) is expressed during the G2/M phase of the cell cycle and associates with microtubules of the mitotic spindle. Increased caspase-3 activity is detected when a disruption of survivinmicrotubule interactions occurs. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
Survivin (N111) polyclonal antibody detects endogenous levels of Survivin protein. Immunogen :
Synthetic peptide, corresponding to amino acids 81-130 of Human Survivin. Conjugate :
Unconjugated Modification :
Unmodification
Survivin (N111) polyclonal antibody Background :
Two mammalian homologs of baculovirus p35, referred to as inhibitor of apoptosis protein (IAP) 1 and 2, share an amino-terminal baculovirus IAP repeat (BIR) motif and a carboxy-terminal RING finger. Although the c-IAPs do not directly associate with the TNF receptor (TNF-R), they efficiently block TNF-mediated apoptosis through their interaction with the downstream TNF-R effectors, TRAF1 and TRAF2. Additional IAP family members include ILP (for IAP-like protein) and survivin. ILP inhibits activated caspase-3, leading to the resistance of FASmediated apoptosis. Survivin (also designated TIAP) is expressed during the G2/M phase of the cell cycle and associates with microtubules of the mitotic spindle. Increased caspase-3 activity is detected when a disruption of survivinmicrotubule interactions occurs. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
Survivin (N111) polyclonal antibody detects endogenous levels of Survivin protein. Immunogen :
Synthetic peptide, corresponding to amino acids 81-130 of Human Survivin. Conjugate :
Unconjugated Modification :
Unmodification
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Western blot (WB) analysis of Survivin (N111) polyclonal antibody at 1:500 dilution Lane1:HEK293T whole cell lysate Lane2:Raw264.7 whole cell lysate Lane3:H9C2 whole cell lysate -
Immunohistochemistry (IHC) analyzes of Survivin (N111) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.
Effect of interaction of magnetic nanoparticles of Fe3O4 and artesunate on apoptosis of K562 cells
PMCID: Pubmed No.:21822380
CIP2A with survivin protein expressions in human non-small-cell lung cancer correlates with prognosis
PMCID: Pubmed No.:21874565
Down-regulation of Notch receptor signaling pathway induces caspase-dependent and caspase-independent apoptosis in lung squamous cell carcinoma cells
PMCID: Pubmed No.:22583356
Down regulation of differentiated embryonic chondrocytes 1 (DEC1) is involved in 8-methoxypsoralen-induced apoptosis in HepG2 cells
PMCID: Pubmed No.:22796345
CDX2 serves as a Wnt signaling inhibitor and is frequently methylated in lung cancer.
PMCID: Pubmed No.:22892849
Synergistic anticancer effects of curcumin and resveratrol in Hepa1-6 hepatocellular carcinoma cells
PMCID: Pubmed No.:23446753
Polymorphisms of Survivin and Its Protein Expression Are Associated with Colorectal Cancer Susceptibility in Chinese Population
PMCID: Pubmed No.:23581553
Rapamycin interacts synergistically with idarubicin to induce T-leukemia cell apoptosis in vitro and in a mesenchymal stem cell simulated drug-resistant microenvironment via Akt/mammalian target of rapamycin and extracellular signal-related kinase signaling pathways
PMCID: Pubmed No.:23741975
Teng-Long-Bu-Zhong-Tang, a Chinese herbal formula, enhances anticancer effects of 5 - Fluorouracil in CT26 colon carcinoma
PMCID: Pubmed No.:23758730
MCM-2 is a therapeutic target of Trichostatin A in colon cancer cells
PMCID: Pubmed No.:23770000
Ran GTPase protein promotes human pancreatic cancer proliferation by deregulating the expression of Survivin and cell cycle proteins
PMCID: Pubmed No.:24076388
Molecular Mechanisms of Exopolysaccharide from Aphanothece halaphytica (EPSAH) Induced Apoptosis in HeLa Cells
PMCID: Pubmed No.:24466342
Small Double-Stranded RNA Mediates the Anti-Cancer Effects of p21WAF1/ClP1 Transcriptional Activation in a Human Glioma Cell Line
PMCID: Pubmed No.:24532499
miR-155 regulates the proliferation and cell cycle of colorectal carcinoma cells by targeting E2F2
PMCID: Pubmed No.:24793496
Effects of infrasound on the growth of bone marrow mesenchymal stem cells: A pilot study
PMCID: Pubmed No.:25175368
Arginine deiminase augments the chemosensitivity of argininosuccinate synthetase-deficient pancreatic cancer cells to gemcitabine via inhibition of NF-kappaB signaling.
PMCID: Pubmed No.:25240403
miR-31 promotes proliferation of colon cancer cells by targeting E2F2
PMCID: Pubmed No.:25362258
Epigenetic changes and functional study of HOXA11 in human gastric cancer
PMCID: Pubmed No.:25590359
"Triple-Punch" Strategy for Triple Negative Breast Cancer Therapy with Minimized Drug Dosage and Improved Antitumor Efficacy.
PMCID: Pubmed No.:25611071
Effects of infrasound on the growth of bone marrow mesenchymal stem cells: A pilot study
PMCID: Pubmed No.:25175368
Arginine deiminase augments the chemosensitivity of argininosuccinate synthetase-deficient pancreatic cancer cells to gemcitabine via inhibition of NF-kappaB signaling.
PMCID: Pubmed No.:25240403
miR-31 promotes proliferation of colon cancer cells by targeting E2F2
PMCID: Pubmed No.:25362258
Ran GTPase protein promotes human pancreatic cancer proliferation by deregulating the expression of Survivin and cell cycle proteins
PMCID: Pubmed No.:24076388
miR-155 regulates the proliferation and cell cycle of colorectal carcinoma cells by targeting E2F2
PMCID: Pubmed No.:24793496
Molecular Mechanisms of Exopolysaccharide from Aphanothece halaphytica (EPSAH) Induced Apoptosis in HeLa Cells
PMCID: Pubmed No.:24466342
Small Double-Stranded RNA Mediates the Anti-Cancer Effects of p21WAF1/ClP1 Transcriptional Activation in a Human Glioma Cell Line
PMCID: Pubmed No.:24532499
Egg Yolk Immunoglobulin Supplementation Prevents Rat Liver from Aflatoxin B1-Induced Oxidative Damage and Genotoxicity
PMCID: Pubmed No.:30449111
Imaging Intracellular Drug/siRNA Co-Delivery by Self-Assembly Cross-Linked Polyethylenimine with Fluorescent Core-Shell Silica Nanoparticles
PMCID: Pubmed No.:35566982
KPT-330 and Y219 exert a synergistic anti-tumor effect in triple-negative breast cancer through inhibiting NF-κB signaling
PMCID: Pubmed No.:36847599
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.
Price/Size :
USD 368/1mg/vial
Tips:
For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).Describe :
Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.Formula:
Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.
Storage:
The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C.
Note :
This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.