Product Name :
Cyclin D1 (L283) polyclonal antibody Background :
Considerable effort directed towards the identification of G1 cyclins has led to the isolation of cyclin D, cyclin C and cyclin E. Of these, cyclin D corresponds to a putative human oncogene, designated PRAD1, which maps at the site of the Bcl1 rearrangement in certain lymphomas and leukemias. Two additional human type D cyclins, as well as their mouse homologs, have been identified. Evidence has established that members of the cyclin D family function to regulate phosphorylation of the retinoblastoma gene product, thereby activating E2F transcription factors. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
Cyclin D1 (L283) polyclonal antibody detects endogenous levels of Cyclin D1 protein. Immunogen :
Synthetic peptide, corresponding to amino acids 250-300 of Human Cyclin D1. Conjugate :
Unconjugated Modification :
Unmodification
Cyclin D1 (L283) polyclonal antibody Background :
Considerable effort directed towards the identification of G1 cyclins has led to the isolation of cyclin D, cyclin C and cyclin E. Of these, cyclin D corresponds to a putative human oncogene, designated PRAD1, which maps at the site of the Bcl1 rearrangement in certain lymphomas and leukemias. Two additional human type D cyclins, as well as their mouse homologs, have been identified. Evidence has established that members of the cyclin D family function to regulate phosphorylation of the retinoblastoma gene product, thereby activating E2F transcription factors. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
Cyclin D1 (L283) polyclonal antibody detects endogenous levels of Cyclin D1 protein. Immunogen :
Synthetic peptide, corresponding to amino acids 250-300 of Human Cyclin D1. Conjugate :
Unconjugated Modification :
Unmodification
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Western blot (WB) analysis of Cyclin D1 (L283) pAb at 1:2000 dilution Lane1:HepG2 whole cell lysate(20ug) Lane2:L02 whole cell lysate(20ug) Lane3:C6 whole cell lysate(20ug) Lane4:3T3-L1 whole cell lysate(20ug)
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Immunohistochemistry (IHC) analyzes of Cyclin D1 (L283) pAb in paraffin-embedded human breast carcinoma tissue at 1:50.
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MicroRNA-302b suppresses cell proliferation by targeting EGFR in human hepatocellular carcinoma SMMC-7721 cells.
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MicroRNA-30b functions as a tumour suppressor in human colorectal cancer by targeting KRAS, PIK3CD and BCL2
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Fangchinoline as a kinase inhibitor targets FAK and suppresses FAK-mediated signaling pathway in A549
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MTA1 downregulation inhibits malignant potential in a small cell lung cancer cell line
PMCID: Pubmed No.:25502548
Water Stress Proteins from Nostoc commune Vauch. Exhibit Anti-Colon Cancer Activities in Vitro and in Vivo
PMCID: Pubmed No.:25524246
Oxymatrine targets EGFRp-Tyr845 and inhibits EGFR-related signaling pathways to suppress the proliferation and invasion of gastric cancer cells
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The role of EGFR/PI3K/Akt/cyclinD1 signaling pathway in acquired middle ear cholesteatoma
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MicroRNA-302b suppresses cell proliferation by targeting EGFR in human hepatocellular carcinoma SMMC-7721 cells.
PMCID: Pubmed No.:24083596
Repression of PES1 expression inhibits growth of gastric cancer
PMCID: Pubmed No.:26423399
Tumorigenic hybrids between mesenchymal stem cells and gastric cancer cells enhanced cancer proliferation, migration and stemness
PMCID: Pubmed No.:26498753
Suppression of RAD21 induces senescence of MDA-MB-231 human breast cancer cells through RB1 pathway activation via c-Myc downregulation.
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GEN-27, a newly synthetic isoflavonoid, inhibits the proliferation of colon cancer cells in inflammation microenvironment by suppressing NF-κB pathway.
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Silencing of HMGA2 suppresses cellular proliferation, migration, invasion, and epithelial–mesenchymal transition in bladder cancer
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PFR peptide, one of the antimicrobial peptides identified from the derivatives of lactoferrin, induces necrosis in leukemia cells
PMCID: Pubmed No.:26860588
Targeted deletion of miR‐139‐5p activates MAPK, NF‐κB and STAT3 signaling and promotes intestinal inflammation and colorectal cancer
PMCID: Pubmed No.:26859226
ZFX Facilitates Cell Proliferation and Imatinib Resistance in Chronic Myeloid Leukemia Cells
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Down-regulating ribonuclease inhibitor enhances metastasis of bladder cancer cells through regulating epithelial-mesenchymal transition and ILK signaling pathway
PMCID: Pubmed No.:24768914
Activation of Mesenchymal Stem Cells by Macrophages Prompts Human Gastric Cancer Growth through NF-κB Pathway
PMCID: Pubmed No.:24824968
MicroRNA-30b functions as a tumour suppressor in human colorectal cancer by targeting KRAS, PIK3CD and BCL2
PMCID: Pubmed No.:24293274
Reconstructed mung bean trypsin inhibitor targeting cell surface GRP78 induces apoptosis and inhibits tumor growth in colorectal cancer
PMCID: Pubmed No.:24333163
Dichlorodiphenyltrichloroethane exposure induces the growth of hepatocellular carcinoma via Wnt/β-catenin pathway
PMCID: Pubmed No.:24355586
PES1 differentially regulates the expression of ERα and ERβ in ovarian cancer
PMCID: Pubmed No.:24376209
Involvement of activating ERK1/2 through G protein coupled receptor 30 and estrogen receptor α/β in low doses of bisphenol A promoting growth of Sertoli TM4 cells
PMCID: Pubmed No.:24495410
PES1 differentially regulates the expression of ERα and ERβ in ovarian cancer
PMCID: Pubmed No.:24376209
Iso-suillin isolated from Suillus luteus, induces G1 phase arrest and apoptosis in human hepatoma SMMC-7721 cells.
PMCID: Pubmed No.:24606477
A novel protein extracted from foxtail millet bran displays anti-carcinogenic effects in human colon cancer cells
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Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.
Price/Size :
USD 368/1mg/vial
Tips:
For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).Describe :
Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.Formula:
Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.
Storage:
The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C.
Note :
This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.