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Cyclin D1 (L283) polyclonal antibody BS1741
  • Western blot (WB) analysis of Cyclin D1 (L283) pAb at 1:2000 dilution Lane1:HepG2 whole cell lysate(20ug) Lane2:L02 whole cell lysate(20ug) Lane3:C6 whole cell lysate(20ug) Lane4:3T3-L1 whole cell lysate(20ug)
  • Immunohistochemistry (IHC) analyzes of Cyclin D1 (L283) pAb in paraffin-embedded human breast carcinoma tissue at 1:50.
Product NameCyclin D1 (L283) polyclonal antibody
Catalog No.BS1741
Swiss-ProtP24385
Host Rabbit
ReactivityHuman,Mouse,Rat
ApplicationsWB IHC
Application_allWB: 1:500~1:1000 IHC: 1:50~1:200
BiowMW~ 36 kDa
Alternative NameG1/S-specific cyclin-D1; B-cell lymphoma 1 protein; BCL-1; BCL-1 oncogene; PRAD1 oncogene; CCND1; BCL1; PRAD1
Purification&PurityThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
ConjugateUnconjugated
ModificationUnmodification
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50ul $158
100ul $275
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Product Name :
Cyclin D1 (L283) polyclonal antibody
Background :
Considerable effort directed towards the identification of G1 cyclins has led to the isolation of cyclin D, cyclin C and cyclin E. Of these, cyclin D corresponds to a putative human oncogene, designated PRAD1, which maps at the site of the Bcl1 rearrangement in certain lymphomas and leukemias. Two additional human type D cyclins, as well as their mouse homologs, have been identified. Evidence has established that members of the cyclin D family function to regulate phosphorylation of the retinoblastoma gene product, thereby activating E2F transcription factors.
Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity :
Cyclin D1 (L283) polyclonal antibody detects endogenous levels of Cyclin D1 protein.
Immunogen :
Synthetic peptide, corresponding to amino acids 250-300 of Human Cyclin D1.
Conjugate :
Unconjugated
Modification :
Unmodification
  • Western blot (WB) analysis of Cyclin D1 (L283) pAb at 1:2000 dilution Lane1:HepG2 whole cell lysate(20ug) Lane2:L02 whole cell lysate(20ug) Lane3:C6 whole cell lysate(20ug) Lane4:3T3-L1 whole cell lysate(20ug)
  • Immunohistochemistry (IHC) analyzes of Cyclin D1 (L283) pAb in paraffin-embedded human breast carcinoma tissue at 1:50.
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Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

Price/Size :

USD 368/1mg/vial



Tips: 

For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).

Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 


Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.
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