Product Name :
Caspase 1 (K372) polyclonal antibody Background :
Caspase-1 (interleukin-1 beta convertase) belongs to the Interleukin-1-beta converting enzyme subfamily of caspases which mediate many features of apoptosis, including structural dismantling of cell bodies and nuclei, fragmentation of genomic DNA, destruction of regulatory proteins and propagation of other pro-apoptotic molecules. Caspase-1 promotes maturation of interleukin IL-1 beta and interleukin 18 (IL-18) by proteolytic cleavage of precursor forms into biologically active pro-inflammatory cytokines. Cleavage of Caspase-1 into the p20 and p10 subunits directly correlates with the progression of apoptosis in the cell resulting in the initiation of the caspase cascade. Proteolytic cleavage of the precursor caspase-1 at glycine residue 317 in human and 315 in mouse generates the functional caspase-1 subunits, known as p20 and p10 subunits. At the amino acid level, the mouse p10 subunit is 81% identical to the human p10 subunit. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
Caspase 1 (K372) polyclonal antibody detects endogenous levels of Caspase 1 protein. Immunogen :
Synthetic peptide, corresponding to amino acids 340-390 of Human Caspase 1. Conjugate :
Unconjugated Modification :
Unmodification
Caspase 1 (K372) polyclonal antibody Background :
Caspase-1 (interleukin-1 beta convertase) belongs to the Interleukin-1-beta converting enzyme subfamily of caspases which mediate many features of apoptosis, including structural dismantling of cell bodies and nuclei, fragmentation of genomic DNA, destruction of regulatory proteins and propagation of other pro-apoptotic molecules. Caspase-1 promotes maturation of interleukin IL-1 beta and interleukin 18 (IL-18) by proteolytic cleavage of precursor forms into biologically active pro-inflammatory cytokines. Cleavage of Caspase-1 into the p20 and p10 subunits directly correlates with the progression of apoptosis in the cell resulting in the initiation of the caspase cascade. Proteolytic cleavage of the precursor caspase-1 at glycine residue 317 in human and 315 in mouse generates the functional caspase-1 subunits, known as p20 and p10 subunits. At the amino acid level, the mouse p10 subunit is 81% identical to the human p10 subunit. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
Caspase 1 (K372) polyclonal antibody detects endogenous levels of Caspase 1 protein. Immunogen :
Synthetic peptide, corresponding to amino acids 340-390 of Human Caspase 1. Conjugate :
Unconjugated Modification :
Unmodification
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Immunohistochemistry (IHC) analyzes of Caspase 1 (K372) pAb in paraffin-embedded human breast carcinoma tissue at 1:50.
Chronic glucocorticoids exposure enhances neurodegeneration in the frontal cortex and hippocampus via NLRP-1 inflammasome activation in male mice
PMCID: Pubmed No.:26434621
Chronic glucocorticoids exposure enhances neurodegeneration in the frontal cortex and hippocampus via NLRP-1 inflammasome activation in male mice
PMCID: Pubmed No.:26434621
Resistance of mitochondrial DNA-depleted cells against oxidized low-density lipoprotein-induced macrophage pyroptosis
PMCID: Pubmed No.:27035880
Chronic glucocorticoids exposure enhances neurodegeneration in the frontal cortex and hippocampus via NLRP-1 inflammasome activation in male mice
PMCID: Pubmed No.:26434621
Resistance of mitochondrial DNA-depleted cells against oxidized low-density lipoprotein-induced macrophage pyroptosis
PMCID: Pubmed No.:27035880
Chronic glucocorticoid exposure activates BK-NLRP1 signal involving in hippocampal neuron damage
PMCID: Pubmed No.:28732502
Esculentoside A exerts anti-inflammatory activity in microglial cells
PMCID: Pubmed No.:28843178
Ginsenoside Rg1 protects against neuronal degeneration induced by chronic dexamethasone treatment by inhibiting NLRP-1 inflammasomes in mice
PMCID: Pubmed No.:28849171
Enterococcus faecalis Lipoteichoic Acid–induced NLRP3 Inflammasome via the Activation of the Nuclear Factor Kappa B Pathway
PMCID: Pubmed No.:27325457
Resistance of mitochondrial DNA-depleted cells against oxidized low-density lipoprotein-induced macrophage pyroptosis
PMCID: Pubmed No.:27035880
Histone Deacetylase 3 Couples Mitochondria to Drive IL-1β-Dependent Inflammation by Configuring Fatty Acid Oxidation
PMCID: Pubmed No.:32937100
Effects of NIX‐mediated mitophagy on ox‐LDL‐induced macrophage pyroptosis in atherosclerosis
PMCID: Pubmed No.:32181963
Propofol Attenuates Inflammatory Damage via Inhibiting NLRP1-Casp1-Casp6 Signaling in Ischemic Brain Injury
PMCID: Pubmed No.:32999158
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.
Price/Size :
USD 368/1mg/vial
Tips:
For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).Describe :
Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.Formula:
Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.
Storage:
The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C.
Note :
This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.