NIFK (D229) polyclonal antibody 
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Product Name :
NIFK (D229) polyclonal antibody Background :
The structural proteins for the complex metalloenzyme nitrogenase include NIFK, NIFD and NIFH. These proteins are all necessary for archaeal and bacterial nitrogen fixation. The NIFK gene encodes the b subunit of the nitrogenase molybdenum-iron (MoFe) tetramer. NIFK localizes to the nucleolus where it interactes with the fork-head associated domain of the proliferation marker protein Ki-67 in a mitosis-specific and phosphorylation-dependent manner. NIFK is widely expressed in adult tissues, suggesting other functions in addition to its interaction with Ki-67, which is only expressed in proliferating cells. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
NIFK (D229) polyclonal antibody detects endogenous levels of NIFK protein. Immunogen :
Synthetic peptide, corresponding to amino acids 196-245 of Human NIFK. Conjugate :
Unconjugated Modification :
Unmodification
NIFK (D229) polyclonal antibody Background :
The structural proteins for the complex metalloenzyme nitrogenase include NIFK, NIFD and NIFH. These proteins are all necessary for archaeal and bacterial nitrogen fixation. The NIFK gene encodes the b subunit of the nitrogenase molybdenum-iron (MoFe) tetramer. NIFK localizes to the nucleolus where it interactes with the fork-head associated domain of the proliferation marker protein Ki-67 in a mitosis-specific and phosphorylation-dependent manner. NIFK is widely expressed in adult tissues, suggesting other functions in addition to its interaction with Ki-67, which is only expressed in proliferating cells. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
NIFK (D229) polyclonal antibody detects endogenous levels of NIFK protein. Immunogen :
Synthetic peptide, corresponding to amino acids 196-245 of Human NIFK. Conjugate :
Unconjugated Modification :
Unmodification
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Western blot (WB) analysis of NIFK (D229) polyclonal antibody at 1:500 dilution Lane1:A549 whole cell lysate Lane2:Raw264.7 whole cell lysate Lane3:H9C2 whole cell lysate -
Immunohistochemistry (IHC) analyzes of NIFK (D229) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.
Price/Size :
USD 368/1mg/vial
Tips:
For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).Describe :
Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.Formula:
Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.
Storage:
The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C.
Note :
This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.