Sp1 (G447) polyclonal antibody 
Datasheet
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Product Name :
Sp1 (G447) polyclonal antibody Background :
Sp1 is a sequence-specific transcription factor that recognizes GGGGCGGGGC and closely related sequences, which are often referred to as GC boxes. Sp1 was initially identified as a HeLa cell-derived factor that selectively activates in vitro transcription from the SV40 promoter and binds to the multiple GC boxes in the 21 bp repeated elements in SV40. The sequence specificity of DNA binding is conferred by Zn (II) fingers, whereas a different region of Sp1 appears to regulate the affinity of DNA binding. Sp1 belongs to a subgroup of transcription factors that are phosphorylated upon binding to promoter sequences. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
SP1 (G447) polyclonal antibody detects endogenous levels of SP1 protein. Immunogen :
Synthetic peptide, corresponding to amino acids 421-470 of Human Sp1. Conjugate :
Unconjugated Modification :
Unmodification
Sp1 (G447) polyclonal antibody Background :
Sp1 is a sequence-specific transcription factor that recognizes GGGGCGGGGC and closely related sequences, which are often referred to as GC boxes. Sp1 was initially identified as a HeLa cell-derived factor that selectively activates in vitro transcription from the SV40 promoter and binds to the multiple GC boxes in the 21 bp repeated elements in SV40. The sequence specificity of DNA binding is conferred by Zn (II) fingers, whereas a different region of Sp1 appears to regulate the affinity of DNA binding. Sp1 belongs to a subgroup of transcription factors that are phosphorylated upon binding to promoter sequences. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
SP1 (G447) polyclonal antibody detects endogenous levels of SP1 protein. Immunogen :
Synthetic peptide, corresponding to amino acids 421-470 of Human Sp1. Conjugate :
Unconjugated Modification :
Unmodification
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Western blot (WB) analysis of Sp1 (G447) polyclonal antibody at 1:500 dilution Lane1:PC12 whole cell lysate(40ug) Lane2:CT26 whole cell lysate(40ug) Lane3:Hela whole cell lysate(40ug) Lane4:COS-7 whole cell lysate(40ug) -
Immunohistochemistry (IHC) analyzes of Sp1 (G447) pAb in paraffin-embedded human breast carcinoma tissue at 1:50.
Heme Oxygenase-1/Carbon Monoxide Induces Vascular Endothelial Growth Factor Expression via p38 Kinase-dependent Activation of Sp1
PMCID: Pubmed No.:21115498
The TGF‐β‐induced up‐regulation of NKG2DLs requires AKT/GSK‐3β‐mediated stabilization of SP1
PMCID: Pubmed No.:28165192
Loss of the clock protein PER2 shortens the erythrocyte life span in mice
PMCID: Pubmed No.:28607147
Expression and role of specificity protein 1 and collagen I in recurrent pterygial tissues
PMCID: Pubmed No.:33614450
Expression of specificity protein 1 and collagen I in primary pterygial tissues
PMCID: Pubmed No.:31166424
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.
Price/Size :
USD 368/1mg/vial
Tips:
For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).Describe :
Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.Formula:
Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.
Storage:
The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C.
Note :
This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.