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JNK1/2/3 (P184) polyclonal antibody BS1544
  • Western blot (WB) analysis of JNK1/2/3 (P184) polyclonal antibody at 1:500 dilution Lane1:HEK293T whole cell lysate Lane2:Raw264.7 whole cell lysate Lane3:H9C2 whole cell lysate
  • Immunohistochemistry (IHC) analyzes of JNK1/2/3 (P184) pAb in paraffin-embedded human breast carcinoma tissue at 1:50.showing cytoplasmic and nucleus staining. Negative control (the right)Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.
  • Immunohistochemistry (IHC) analyzes of JNK1/2/3 (P184) pAb in paraffin-embedded human breast carcinoma tissue at 1:50.showing cytoplasmic and nucleus staining. Negative control (the right)Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.
  • Immunohistochemistry (IHC) analyzes of JNK1/2/3 (P184) pAb in paraffin-embedded human breast carcinoma tissue at 1:50.showing cytoplasmic and nucleus staining. Negative control (the right)Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.
Product NameJNK1/2/3 (P184) polyclonal antibody
Catalog No.BS1544
Swiss-ProtP45983/P45984/P53779
Host Rabbit
ReactivityHuman,Mouse,Rat
ApplicationsWB IHC IF
Application_allWB: 1:500~1:1000 IHC: 1:50~1:200 IF: 1:50~1:200
BiowMW~ 46,54 kDa
Alternative NameJNK 46; JNK 55; JNK; JNK1; JNK1A2; JNK2; JNK21B1/2; JNK2ALPHA; JNK2B; JNK2BETA; JNK3; JNK3A; MAP kinase 8; MAP kinase 9; MAP kinase p49 3F12; MAPK 8; MAPK 9; MAPK10; MAPK8; MAPK9; MGC50974; Mitogen activated protein kinase 10; Mitogen activated protein kinase 8; Mitogen activated protein kinase 9;
Purification&PurityThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
ConjugateUnconjugated
ModificationUnmodification
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50ul $158
100ul $275
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Product Name :
JNK1/2/3 (P184) polyclonal antibody
Background :
The human JNK1 (PRKM8, SAPK1, MAPK8) gene maps to chromosome 10q11.22 and shares 83% amino acid identity with JNK2. JNK1 is necessary for normal activation and differentiation of CD4 helper T (TH) cells into TH1 and TH2 effector cells. Capsaicin activates JNK1 and p38 in Ras-transformed human breast epithelial cells. Nitrogen oxides (NOx) upregulate JNK1 in addition to c-Fos, c-Jun and other signaling kinases, including MEKK1 and p38. JNK3 (MK10, MAPK10, PRKM10) is activated by pro-inflammatory cytokines and environmental stresss by phosphorylating transcription factors such as c-Jun and ATF2. This is important for AP-1 transcriptional activity regulation. JNK3 is crucial for neuronal apoptosis (stress-induced).
Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity :
JNK1/2/3 (T178) polyclonal antibody detects endogenous levels of JNK1/2/3 protein.
Immunogen :
Synthetic peptide, corresponding to amino acids 150-200 of Human JNK1.
Conjugate :
Unconjugated
Modification :
Unmodification
  • Western blot (WB) analysis of JNK1/2/3 (P184) polyclonal antibody at 1:500 dilution Lane1:HEK293T whole cell lysate Lane2:Raw264.7 whole cell lysate Lane3:H9C2 whole cell lysate
  • Immunohistochemistry (IHC) analyzes of JNK1/2/3 (P184) pAb in paraffin-embedded human breast carcinoma tissue at 1:50.showing cytoplasmic and nucleus staining. Negative control (the right)Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.
  • Immunohistochemistry (IHC) analyzes of JNK1/2/3 (P184) pAb in paraffin-embedded human breast carcinoma tissue at 1:50.showing cytoplasmic and nucleus staining. Negative control (the right)Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.
  • Immunohistochemistry (IHC) analyzes of JNK1/2/3 (P184) pAb in paraffin-embedded human breast carcinoma tissue at 1:50.showing cytoplasmic and nucleus staining. Negative control (the right)Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.
Endothelial cells dysfunction induced by silica nanoparticles through oxidative stress via JNK/P53 and NF-kB pathways PMCID:    Pubmed No.:20727582 Involvement of JNK and P53 Activation in G2/M Cell Cycle Arrest and Apoptosis induced by Titanium Dioxide Nanoparticles in neuron cells PMCID:    Pubmed No.:20863874 c-Jun N-terminal kinase is involved in the regulation of proliferation and apoptosis by integrin-linked kinase in human retinoblastoma cells PMCID:    Pubmed No.:21234586 Luteolin induced G2 phase cell cycle arrest and apoptosis on non-small cell lung cancer cells PMCID:    Pubmed No.:21601631 ROS-activated p38 MAPK/ERK-Akt cascade plays a central role in palmitic acid-stimulated hepatocyte proliferation. PMCID:    Pubmed No.:21620957 Subcellular proteomics: Determination of specific location and expression levels of lymphatic metastasis associated proteins in hepatocellular carcinoma by subcellular fractionation PMCID:    Pubmed No.:21741201 XJP-1, a novel ACEI, with anti-inflammatory properties in HUVECs PMCID:    Pubmed No.:21803355 Hydrogen Sulfide Protects against Chemical Hypoxia-Induced Injury by Inhibiting ROS-Activated ERK1/2 and p38MAPK Signaling Pathways in PC12 Cells PMCID:    Pubmed No.:21998720 Insulin-like growth factor 1 enhances the proliferation and osteogenic differentiation of human periodontal ligament stem cells via ERK and JNK MAPK pathways PMCID:    Pubmed No.:22227802 Disruption of mindin exacerbates cardiac hypertrophy and fibrosis PMCID:    Pubmed No.:22367478 Toosendanin induces apoptosis through suppression of JNK signaling pathway in HL-60 cells PMCID:    Pubmed No.:23111283 Dietary flavonoid genistein induces Nrf2 and phase II detoxification gene expression via ERKs and PKC pathways and protects against oxidative stress in Caco-2 cells PMCID:    Pubmed No.:23255485 Proteomic analysis of novel targets associated with TrkA-mediated tyrosine phosphorylation signaling pathways in SK-N-MC neuroblastoma cells PMCID:    Pubmed No.:23319303 Identification of oligomer proanthocyanidins (F2) isolated from grape seeds as a formyl peptide receptor 1 partial agonist PMCID:    Pubmed No.:23523627 5,7-Dihydroxyflavone Enhances the Apoptosis-Inducing Potential of TRAIL in Human Tumor Cells via Regulation of Apoptosis-Related Proteins PMCID:    Pubmed No.:23533482 Electromagnetic-pulse-induced activation of p38 MAPK pathway and disruption of blood–retinal barrier PMCID:    Pubmed No.:23570913 Dihydrotestosterone alters urocortin levels in human umbilical vein endothelial cells. PMCID:    Pubmed No.:23801677 An Apple Oligogalactan Potentiates the Growth Inhibitory Effect of Celecoxib on Colorectal Cancer PMCID:    Pubmed No.:24274457 Rho kinase inhibition by fasudil suppresses lipopolysaccharide-induced apoptosis of rat pulmonary microvascular endothelial cells via JNK and p38 MAPK pathway PMCID:    Pubmed No.:24406296 Protective effects of the total saponins from Dioscorea nipponica Makino against carbon tetrachloride-induced liver injury in mice through suppression of apoptosis and inflammation PMCID:    Pubmed No.:24491258 Dentin matrix proteins (DMPs) enhance differentiation of BMMSCs via ERK and P38 MAPK pathways PMCID:    Pubmed No.:24562313 ZnO nanoparticle-induced oxidative stress triggers apoptosis by activating JNK signaling pathway in cultured primary astrocytes PMCID:    Pubmed No.:24624962 Long-term blue light exposure induces RGC-5 cell death in vitro: involvement of mitochondria-dependent apoptosis, oxidative stress, and MAPK signaling pathways PMCID:    Pubmed No.:24682673 Effects of the Total Saponins from Rosa laevigata Michx Fruit against Acetaminophen-Induced Liver Damage in Mice via Induction of Autophagy and Suppression of Inflammation and Apoptosis. PMCID:    Pubmed No.:24886943 Berberine inhibits LPS-induced TF procoagulant activity and expression through NF-κB/p65, Akt and MAPK pathway in THP-1 cells PMCID:    Pubmed No.:24905527 Mitofusin 2 deficiency leads to oxidative stress that contributes to insulin resistance in rat skeletal muscle cells PMCID:    Pubmed No.:25034891 PM2. 5‐induced oxidative stress increases adhesion molecules expression in human endothelial cells through the ERK/AKT/NF‐κB‐dependent pathway PMCID:    Pubmed No.:25876056 An Apple Oligogalactan Potentiates the Growth Inhibitory Effect of Celecoxib on Colorectal Cancer PMCID:    Pubmed No.:24274457 Rho kinase inhibition by fasudil suppresses lipopolysaccharide-induced apoptosis of rat pulmonary microvascular endothelial cells via JNK and p38 MAPK pathway PMCID:    Pubmed No.:24406296 Protective effects of the total saponins from Dioscorea nipponica Makino against carbon tetrachloride-induced liver injury in mice through suppression of apoptosis and inflammation PMCID:    Pubmed No.:24491258 Dentin matrix proteins (DMPs) enhance differentiation of BMMSCs via ERK and P38 MAPK pathways PMCID:    Pubmed No.:24562313 ZnO nanoparticle-induced oxidative stress triggers apoptosis by activating JNK signaling pathway in cultured primary astrocytes PMCID:    Pubmed No.:24624962 Long-term blue light exposure induces RGC-5 cell death in vitro: involvement of mitochondria-dependent apoptosis, oxidative stress, and MAPK signaling pathways PMCID:    Pubmed No.:24682673 Fenofibrate improves high-fat diet-induced and palmitate-induced endoplasmic reticulum stress and inflammation in skeletal muscle PMCID:    Pubmed No.:27297630 Regulation of RAW 264.7 cell-mediated immunity by polysaccharides from Agaricus blazei Murill via the MAPK signal transduction pathway PMCID:    Pubmed No.:28322382 Downregulation of Foxc2 enhances apoptosis induced by 5-fluorouracil through activation of MAPK and AKT pathways in colorectal cancer PMCID:    Pubmed No.:26893778 Sitagliptin Reduces Endothelial Dysfunction and Apoptosis Induced by High-Fat Diet and Palmitate in Thoracic Aortas and Endothelial Cells via ROS-ER Stress-CHOP Pathway PMCID:    Pubmed No.:34531738 Downregulation of Siah1 promotes colorectal cancer cell proliferation and migration by regulating AKT and YAP ubiquitylation and proteasome degradation PMCID:    Pubmed No.:32082080 Protective effects of Danzhi jiangtang capsule on vascular endothelial damages induced by high-fat diet and palmitic acid PMCID:    Pubmed No.:30257381 Curcumin restrains hepatic glucose production by blocking cAMP/PKA signaling and reducing acetyl CoA accumulation in high-fat diet (HFD)-fed mice PMCID:    Pubmed No.:29499209 Heat stress during late pregnancy of sows influences offspring longissimus dorsi muscle growth at weaning PMCID:    Pubmed No.:33765607 Anti-Cancer Effects of Sulfasalazine and Vitamin E Succinate in MDA-MB 231 Triple-Negative Breast Cancer Cells PMCID:    Pubmed No.:31171899 Inhibition of the antigen-presenting ability of dendritic cells by non-structural protein 2 of influenza A virus PMCID:    Pubmed No.:35259598 Muscone and (+)-Borneol Cooperatively Strengthen CREB Induction of Claudin 5 in IL-1β-Induced Endothelium Injury PMCID:    Pubmed No.:35892657
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

Price/Size :

USD 368/1mg/vial



Tips: 

For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).

Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 


Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.
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