Product Name :
ERα (K531) polyclonal antibody Background :
Estrogen receptors (ER) are members of the steroid/thyroid hormone receptor uperfamily of ligand-activated transcription factors. Estrogen receptors, ncluding ERα and ERβ, contain DNA binding and ligand binding domains nd are critically involved in regulating the normal function of reproductive issues. ERα and ERβ have been shown to be differentially activated by various igands. Receptor-ligand interactions trigger a cascade of events, including issociation from heat shock proteins, receptor dimerization, phosphorylation nd the association of the hormone activated receptor with specific regulatory lements in target genes. Evidence suggests that ERα and ERβ may be egulated by distinct mechanisms even though they share many functional characteristics. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
ER-α (K531) polyclonal antibody detects endogenous levels of ER-α protein. Immunogen :
Synthetic peptide, corresponding to amino acids 501-550 of Human ERα. Conjugate :
Unconjugated Modification :
Unmodification
ERα (K531) polyclonal antibody Background :
Estrogen receptors (ER) are members of the steroid/thyroid hormone receptor uperfamily of ligand-activated transcription factors. Estrogen receptors, ncluding ERα and ERβ, contain DNA binding and ligand binding domains nd are critically involved in regulating the normal function of reproductive issues. ERα and ERβ have been shown to be differentially activated by various igands. Receptor-ligand interactions trigger a cascade of events, including issociation from heat shock proteins, receptor dimerization, phosphorylation nd the association of the hormone activated receptor with specific regulatory lements in target genes. Evidence suggests that ERα and ERβ may be egulated by distinct mechanisms even though they share many functional characteristics. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
ER-α (K531) polyclonal antibody detects endogenous levels of ER-α protein. Immunogen :
Synthetic peptide, corresponding to amino acids 501-550 of Human ERα. Conjugate :
Unconjugated Modification :
Unmodification
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Immunohistochemistry (IHC) analyzes of ERα (K531) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.
Estrogen Receptor and PI3K/Akt Signaling Pathway Involvement in S-(-) Equol-Induced Activation of Nrf2/ARE in Endothelial Cells
PMCID: Pubmed No.:24260155
Mechanisms of Gefitinib-mediated reversal of tamoxifen resistance in MCF-7 breast cancer cells by inducing ERα re-expression
PMCID: Pubmed No.:25644501
Estrogen Receptor and PI3K/Akt Signaling Pathway Involvement in S-(-) Equol-Induced Activation of Nrf2/ARE in Endothelial Cells
PMCID: Pubmed No.:24260155
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.
Price/Size :
USD 368/1mg/vial
Tips:
For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).Describe :
Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.Formula:
Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.
Storage:
The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C.
Note :
This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.