Ubiquitin (A46) polyclonal antibody 
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Product Name :
Ubiquitin (A46) polyclonal antibody Background :
Ubiquitin (Ub) is among the most phylogenetically conserved proteins known. The primary function of ubiquitin is to clear abnormal, foreign and improperly folded proteins by targeting them for degradation by the 26S proteosome. This small, 76 amino acid protein can be covalently attached to cellular proteins via an isopeptide linkage between the carboxy terminal group of ubiquitin and lysine amino groups on the acceptor protein. For proteolysis to occur, ubiquitin oligomers must be assembled. Ubiquitin chains on proteolytic substrates are commonly found to have an isopeptide bridge between Lysine 48 of one ubiquitin molecule and the carboxy-terminus of a neighboring ubiquitin molecule. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
Ubiquitin (A46) polyclonal antibody detects ubiquitin, polyubiquitin and ubiquitinated proteins. Immunogen :
Synthetic peptide, corresponding to amino acids 20-60 of Ubiquitin. Conjugate :
Unconjugated Modification :
Unmodification
Ubiquitin (A46) polyclonal antibody Background :
Ubiquitin (Ub) is among the most phylogenetically conserved proteins known. The primary function of ubiquitin is to clear abnormal, foreign and improperly folded proteins by targeting them for degradation by the 26S proteosome. This small, 76 amino acid protein can be covalently attached to cellular proteins via an isopeptide linkage between the carboxy terminal group of ubiquitin and lysine amino groups on the acceptor protein. For proteolysis to occur, ubiquitin oligomers must be assembled. Ubiquitin chains on proteolytic substrates are commonly found to have an isopeptide bridge between Lysine 48 of one ubiquitin molecule and the carboxy-terminus of a neighboring ubiquitin molecule. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
Ubiquitin (A46) polyclonal antibody detects ubiquitin, polyubiquitin and ubiquitinated proteins. Immunogen :
Synthetic peptide, corresponding to amino acids 20-60 of Ubiquitin. Conjugate :
Unconjugated Modification :
Unmodification
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Western blot (WB) analysis of Ubiquitin (A46) polyclonal antibody at 1:500 dilution Lane1:Hela cell lysate treated with UV Lane2:HEK293T cell ysate Lane3:NIH-3T3 cell lysate treated with UV Lane4:Rat kidney tissue lysate -
Immunohistochemistry (IHC) analyzes of Ubiquitin (A46) pAb in paraffin-embedded human breast carcinoma tissue.
p28GANK Overexpression Accelerates Hepatocellular Carcinoma Invasiveness and Metastasis via Phosphoinositol 3-Kinase/AKT/Hypoxia-Inducible Factor-1a Pathways
PMCID: Pubmed No.:21254169
Gambogic acid-induced degradation of mutant p53 is mediated by proteasome and related to CHIP
PMCID: Pubmed No.:21268072
Heat shock protein gp96 decreases p53 stability by regulating Mdm2 E3 ligase activity in liver cancer
PMCID: Pubmed No.:25637791
Claudin-4 is required for modulation of paracellular permeability by muscarinic acetylcholine receptor in epithelial cells
PMCID: Pubmed No.:25948584
The Nature of the N-Terminal Amino Acid Residue of HIV-1 RNase H Is Critical for the Stability of Reverse Transcriptase in Viral Particles
PMCID: Pubmed No.:25392207
Downregulation of the ubiquitin ligase KBTBD8 prevented epithelial ovarian cancer progression
PMCID: Pubmed No.:33109073
FBXW24 controls female meiotic prophase progression by regulating SYCP3 ubiquitination
PMCID: Pubmed No.:35858239
Myocardial structure and functional alterations in a preclinical model of exertional heat stroke
PMCID: Pubmed No.:37004732
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.
Price/Size :
USD 368/1mg/vial
Tips:
For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).Describe :
Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.Formula:
Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.
Storage:
The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C.
Note :
This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.