Product Name :
SAE2 (S621) polyclonal antibody Background :
The human UBiquitin Activating domain 2 (UBA2) gene is located on chromosome 19q12. Human UBA2 could form a beta mercaptoethanol-sensitive conjugate with members of the sentrin family, but not with ubiquitin of NEDD8 in the presence of AOS1. The recombinant proteins, Sua1p and hUba2, form a complex. In this complex, hUba2 binds SUMO-1, which gives the complex the activity of the SUMO-1 activating enzyme. Therefore, UBA2 has been found to be a component of the sentrin-activating enzyme complex. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
Uba2 (S621) polyclonal antibody detects endogenous levels of Uba2 protein. Immunogen :
Synthetic peptide, corresponding to amino acids 598-640 of Human SAE2. Conjugate :
Unconjugated Modification :
Unmodification

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  Western blot (WB) analysis of SAE2 (S621) polyclonal antibody at 1:500 dilution Lane1:A549 whole cell lysate(40ug) Lane2:HEK293T whole cell lysate(40ug) Lane3:H1792 whole cell lysate(40ug) Lane4:PC12 whole cell lysate(40ug) Lane5:BV2 whole cell lysate(40ug)
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  Immunohistochemistry (IHC) analyzes of SAE2 (S621) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.

Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

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Price/Size :

USD 368/1mg/vial

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Tips: 

For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).

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Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

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Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

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Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 

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Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.

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