Product Name :
Tubulin γ (F414) polyclonal antibody Background :
TUB4, the gene for Saccharomyces cerevisiae γ-Tubulin, encodes a 473 amino acid structural protein that localizes to the spindle pole body. γ Tubulin, an essential protein for cell growth, organizes microtubule arrays in the nucleus and cytoplasm. γ Tubulin-depleted cells fail to form functional spindles and arrest during nuclear division. γ Tubulin associates with spindle body components Spc97 and Spc98 to form the γ Tubulin complex. The budding yeast γ Tubulin complex contains one molecule each of Spc97 and Spc98 and two molecules of γ Tubulin. In the SPB, Spc110 binds Spc97 and Spc98 of the γ Tubulin complex. 2D gel analysis indicates five isoforms of γ Tubulin. The phosphorylation of γ Tubulin at Tyr 445 plays a regulatory role in microtubule formation. The incidence rate for this phosphorylation event peaks during G1. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
Tubulin γ (F414) polyclonal antibody detects endogenous levels of Tubulin γ protein. Immunogen :
Synthetic peptide, corresponding to amino acids 380-430 of Human Tubulin γ. Conjugate :
Unconjugated Modification :
Unmodification

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  Immunohistochemistry (IHC) analyzes of Tubulin γ (F414) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.

Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

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Price/Size :

USD 368/1mg/vial

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Tips: 

For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).

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Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

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Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

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Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 

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Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.

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