Product Name :
TRADD (L282) polyclonal antibody Background :
TRADD (TNF R1 associated death domain) is a 34kDa protein that associates with the death domains of both FAS and TNFR1 via their DDH regions. Over expression of TRADD leads to NFkB activation and apoptosis in the absence of TNF. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
TRADD (L282) polyclonal antibody detects endogenous levels of TRADD protein. Immunogen :
Synthetic peptide, corresponding to amino acids 250-304 of Human TRADD. Conjugate :
Unconjugated Modification :
Unmodification

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  Western blot (WB) analysis of TRADD (L282) pAb at 1:500 dilution Lane1:PC12 whole cell lysate(20ug) Lane2:AML-12 whole cell lysate(20ug) Lane3:MCF-7 whole cell lysate(40ug) Lane4:HEK293T whole cell lysate(40ug) Lane5:U-87MG whole cell lysate(40ug) Lane6:HepG2 whole cell lysate(40ug) Lane7:Hela whole cell lysate(40ug)
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  Immunohistochemistry (IHC) analyzes of TRADD (L282) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.

Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

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Price/Size :

USD 368/1mg/vial

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Tips: 

For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).

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Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

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Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

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Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 

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Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.

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