Product Name :
NUB1 (Y601) polyclonal antibody Background :
NEDD8 is a ubiquitin-like protein that controls vital biological events through its conjugation to cullin proteins. NEDD8 ultimate buster-1 (NUB1), is a negative regulator of the NEDD8 system that recruits NEDD8 and its conjugates to the proteasome for degradation. It is, therefore a cell growth regulator. The UBA domain of NUB1 is the specific acceptor for the linear ubiquitin precursor. NUB1 is composed of 601 amino acids. It is an interferon-inducible protein and predominantly localizes in the nucleus. NUB1 is specifically expressed in adult human testis, ovary, heart, and skeletal muscle tissues. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
NUB1 (Y601) polyclonal antibody detects endogenous levels of NUB1 protein. Immunogen :
Synthetic peptide, corresponding to amino acids 570-621 of Human NUB1. Conjugate :
Unconjugated Modification :
Unmodification

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  Western blot (WB) analysis of NUB1 (Y601) pAb at 1:500 dilution Lane1:HEK293T whole cell lysate(10ug) Lane2:A549 whole cell lysate(10ug) Lane3:U-87MG whole cell lysate(10ug) Lane4:MCF-7 whole cell lysate(10ug) Lane5:PC3 whole cell lysate(10ug) Lane6:PC12 whole cell lysate(40ug) Lane7:AML-12 whole cell lysate(40ug)
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  Immunohistochemistry (IHC) analyzes of NUB1 (Y601) pAb in paraffin-embedded human tonsil tissue.
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  Immunohistochemistry (IHC) analyzes of NUB1 (Y601) pAb in paraffin-embedded human tonsil tissue.

Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

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Price/Size :

USD 368/1mg/vial

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Tips: 

For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).

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Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

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Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

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Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 

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Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.

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