Product Name :
PLC γ2 (R747) polyclonal antibody Background :
Phosphoinositide-specific phospholipase C (PLC) plays a crucial role in the initiation of receptor mediated signal transduction through the generation of the two second messengers, inositol 1,4,5-triphosphate and diacylglycerol from phosphatidylinositol 4,5-bisphosphate. There are many mammalian PLC isozymes, including PLC β1, PLC β2, PLC β3, PLC β4, PLCγ1, PLCγ2, PLC δ1, PLC δ2 and PLCe. PLCδ exists as four different isoforms. PLC δ1, a calcium signal amplifier, is activated by an atypical GTP-binding protein. In addition, PLC δ1 is an effector for GTP-binding protein transglutaminase II-mediated oxytocin receptor and α1B-adrenoreceptor signaling. Mouse PLC δ1 is highly expressed in brain, heart, lung and testis. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
PLC γ2 (R747) polyclonal antibody detects endogenous levels of PLC γ2 protein. Immunogen :
Synthetic peptide, corresponding to amino acids 720-770 of Human PLC γ2. Conjugate :
Unconjugated Modification :
Unmodification

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  Western blot (WB) analysis of PLC γ2 (R747) pAb at 1:500 dilution Lane1:PC3 whole cell lysate(40ug) Lane2:BV2 whole cell lysate(40ug) Lane3:Hela whole cell lysate(40ug)
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  Immunohistochemistry (IHC) analyzes of PLC γ2 (R747) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.

Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

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Price/Size :

USD 368/1mg/vial

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Tips: 

For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).

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Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

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Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

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Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 

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Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.

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