GSK3β (S2) polyclonal antibody 
Datasheet
COA
MSDS
SHIP
Product Name :
GSK3β (S2) polyclonal antibody Background :
Glycogen synthase kinase 3, or GSK-3, is a serine/threonine, proline-directed kinase involved in a diverse array of signaling pathways, including glycogen synthesis and cellular adhesion, and has been implicated in Alzheimer’s disease. Two forms of GSK-3, designated GSK-3α and GSK-3β, have been identified and differ in their subcellular localization. Tau, a microtubule-binding protein which serves to stabilize microtubules in growing axons, is found to be hyper-phosphorylated in paired helical filaments (PHF), the major fibrous component of neurofibrillary lesions associated with Alzheimer’s disease. Hyperphosphorylation of Tau is thought to be the critical event leading to the assembly of PHF. Six Tau protein isoforms have been identified, all of which are phosphorylated by GSK-3. This presents the possibility that miscues in GSK-3 signaling contribute to the onset of Alzheimer’s disease. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
GSK3β (S2) polyclonal antibody detects endogenous levels of GSK3β protein. Immunogen :
Synthetic peptide, corresponding to amino acids N-terminus of Human GSK3β. Conjugate :
Unconjugated Modification :
Unmodification
GSK3β (S2) polyclonal antibody Background :
Glycogen synthase kinase 3, or GSK-3, is a serine/threonine, proline-directed kinase involved in a diverse array of signaling pathways, including glycogen synthesis and cellular adhesion, and has been implicated in Alzheimer’s disease. Two forms of GSK-3, designated GSK-3α and GSK-3β, have been identified and differ in their subcellular localization. Tau, a microtubule-binding protein which serves to stabilize microtubules in growing axons, is found to be hyper-phosphorylated in paired helical filaments (PHF), the major fibrous component of neurofibrillary lesions associated with Alzheimer’s disease. Hyperphosphorylation of Tau is thought to be the critical event leading to the assembly of PHF. Six Tau protein isoforms have been identified, all of which are phosphorylated by GSK-3. This presents the possibility that miscues in GSK-3 signaling contribute to the onset of Alzheimer’s disease. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
GSK3β (S2) polyclonal antibody detects endogenous levels of GSK3β protein. Immunogen :
Synthetic peptide, corresponding to amino acids N-terminus of Human GSK3β. Conjugate :
Unconjugated Modification :
Unmodification
-
Western blot (WB) analysis of GSK3β (S2) polyclonal antibody at 1:500 dilution Lane1:H9C2 whole cell lysate(40ug) Lane2:CT26 whole cell lysate(40ug) Lane3:COS-7 whole cell lysate(40ug) Lane4:L02 whole cell lysate(40ug) Lane5:HEK293T whole cell lysate(40ug) -
Immunohistochemistry (IHC) analyzes of GSK3β (S2) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.
Hepatitis C virus core protein interacts with Snail and histone deacetylases to promote the metastasis of hepatocellular carcinoma
PMCID: Pubmed No.:26549030
Roles of Wnt/b-catenin signaling in epithelial differentiation of mesenchymal stem cells
PMCID: Pubmed No.:19879238
Astrocyte-elevated gene-1 overexpression is associated with poor prognosis in gastric cancer
PMCID: Pubmed No.:20300973
HOXB7 as a prognostic factor and mediator of colorectal cancer progression
PMCID: Pubmed No.:21474578
SHAFTS: A Hybrid Approach for 3D Molecular Similarity Calculation. 2. Prospective Case Study in the Discovery of Diverse p90 Ribosomal S6 Protein Kinase 2 Inhibitors To Suppress Cell Migration
PMCID: Pubmed No.:21488662
miR-145 inhibits isoproterenol-induced cardiomyocyte hypertrophy by targeting the expression and localization of GATA6.
PMCID: Pubmed No.:23624080
β-Catenin overexpression is associated with gefitinib resistance in non-small cell lung cancer cells
PMCID: Pubmed No.:23707949
sFRP1 Inhibits Epithelial–Mesenchymal Transition in A549 Human Lung Adenocarcinoma Cell Line
PMCID: Pubmed No.:23802127
GSK3β/β-catenin signaling is correlated with the differentiation of glioma cells induced by wogonin
PMCID: Pubmed No.:23872260
Wogonin induced G1 cell cycle arrest by regulating Wnt/β-catenin signaling pathway and inactivating CDK8 in human colorectal cancer carcinoma cells
PMCID: Pubmed No.:23907061
Xanthoceraside attenuates tau hyperphosphorylation and cognitive deficits in intracerebroventricular-streptozotocin injected rats
PMCID: Pubmed No.:23958944
The correlation between TSC2 and GSK3β levels, and outcomes of patients with hepatocellular carcinoma treated by hepatectomy
PMCID: Pubmed No.:24119083
Effect of naringenin on brain insulin signaling and cognitive functions in ICV-STZ induced dementia model of rats
PMCID: Pubmed No.:24337945
Akt Mediates GSK-3β Phosphorylation in the Rat Prefrontal Cortex during the Process of Ketamine Exerting Rapid Antidepressant Actions
PMCID: Pubmed No.:24504086
Role of NMDA receptors in noise-induced tau hyperphosphorylation in rat hippocampus and prefrontal cortex
PMCID: Pubmed No.:24685355
Proteasome Inhibition-Induced Downregulation of Akt/GSK-3β Pathway Contributes to Abnormality of Tau in Hippocampal Slice
PMCID: Pubmed No.:24752588
Effect of Tenuifoliside A isolated from Polygala tenuifolia on the ERK and PI3K pathways in C6 glioma cells.
PMCID: Pubmed No.:24877714
Pretreatment by Evodiamine is Neuroprotective in Cerebral Ischemia: Up-Regulated pAkt, pGSK3β, Down-Regulated NF-κB Expression, and Ameliorated BBB Permeability.
PMCID: Pubmed No.:24919817
Suppression of epithelial-mesenchymal transition and apoptotic pathways by miR-294/302 family synergistically blocks let-7-induced silencing of self-renewal in embryonic stem cells.
PMCID: Pubmed No.:25501598
Fangchinoline targets PI3K and suppresses PI3K/AKT signaling pathway in SGC7901 cells
PMCID: Pubmed No.:25872479
Nicorandil inhibits oxidative stress and amyloid-β precursor protein processing in SH-SY5Y cells overexpressing APPsw.
PMCID: Pubmed No.:25932125
MicroRNA-137 Contributes to Dampened Tumorigenesis in Human Gastric Cancer by Targeting AKT2
PMCID: Pubmed No.:26102366
DGAEE, a newly synthesized derivative of glycyrrhetinic acid, potently attenuates mouse septic shock via its main metabolite DGA in an IL-10-dependent manner
PMCID: Pubmed No.:26456500
Effects of lentivirus-mediated shRNA targeting integrin-linked kinase on oral squamous cell carcinoma in vitro and in vivo
PMCID: Pubmed No.:26531674
The effect and mechanism of bufalin on regulating hepatocellular carcinoma cell invasion and metastasis via Wnt/β-catenin signaling pathway
PMCID: Pubmed No.:26648168
Oroxylin A inhibits invasion and migration through suppressing ERK/GSK‐3β signaling in snail‐expressing non‐small‐cell lung cancer cells
PMCID: Pubmed No.:26741501
Dietary cholesterol promotes AOM-induced colorectal cancer through activating the NLRP3 inflammasome
PMCID: Pubmed No.:26921636
Suppression of epithelial-mesenchymal transition and apoptotic pathways by miR-294/302 family synergistically blocks let-7-induced silencing of self-renewal in embryonic stem cells.
PMCID: Pubmed No.:25501598
The correlation between TSC2 and GSK3β levels, and outcomes of patients with hepatocellular carcinoma treated by hepatectomy
PMCID: Pubmed No.:24119083
DGAEE, a newly synthesized derivative of glycyrrhetinic acid, potently attenuates mouse septic shock via its main metabolite DGA in an IL-10-dependent manner
PMCID: Pubmed No.:26456500
Effects of lentivirus-mediated shRNA targeting integrin-linked kinase on oral squamous cell carcinoma in vitro and in vivo
PMCID: Pubmed No.:26531674
The effect and mechanism of bufalin on regulating hepatocellular carcinoma cell invasion and metastasis via Wnt/β-catenin signaling pathway
PMCID: Pubmed No.:26648168
Oroxylin A inhibits invasion and migration through suppressing ERK/GSK‐3β signaling in snail‐expressing non‐small‐cell lung cancer cells
PMCID: Pubmed No.:26741501
Dietary cholesterol promotes AOM-induced colorectal cancer through activating the NLRP3 inflammasome
PMCID: Pubmed No.:26921636
Proteasome Inhibition-Induced Downregulation of Akt/GSK-3β Pathway Contributes to Abnormality of Tau in Hippocampal Slice
PMCID: Pubmed No.:24752588
Effect of naringenin on brain insulin signaling and cognitive functions in ICV-STZ induced dementia model of rats
PMCID: Pubmed No.:24337945
Akt Mediates GSK-3β Phosphorylation in the Rat Prefrontal Cortex during the Process of Ketamine Exerting Rapid Antidepressant Actions
PMCID: Pubmed No.:24504086
Role of NMDA receptors in noise-induced tau hyperphosphorylation in rat hippocampus and prefrontal cortex
PMCID: Pubmed No.:24685355
Mindin regulates vascular smooth muscle cell phenotype and prevents neointima formation
PMCID: Pubmed No.:25751394
Dietary cholesterol promotes AOM-induced colorectal cancer through activating the NLRP3 inflammasome
PMCID: Pubmed No.:26921636
Vaspin protects against LPS‑induced ARDS by inhibiting inflammation, apoptosis and reactive oxygen species generation in pulmonary endothelial cells via the Akt/GSK‑3β pathway
PMCID: Pubmed No.:29039444
Kai-Xin-San, a traditional Chinese medicine formulation, exerts antidepressive and neuroprotective effects by promoting pCREB upstream pathways
PMCID: Pubmed No.:27882154
Chemopreventive activity of GEN-27, a genistein derivative, in colitis-associated cancer is mediated by p65-CDX2-β-catenin axis
PMCID: Pubmed No.:26910375
DIFFERENTIATED EMBRYONIC CHONDROCYTE EXPRESSED GENE-1 IS A CENTRAL SIGNALING COMPONENT IN THE DEVELOPMENT OF COLLAGEN-INDUCED RHEUMATOID ARTHRITIS
PMCID: Pubmed No.:36739947
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.
Price/Size :
USD 368/1mg/vial
Tips:
For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).Describe :
Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.Formula:
Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.
Storage:
The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C.
Note :
This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.