Product Name :
Amylin (H28) polyclonal antibody Background :
Adrenomedullin (AM), alpha- and beta-calcitonin gene-related peptide(CGRP), calcitonin (CT), and amylin are homologous polypeptides with overlapping biological actions such as vasodilatation and inhibition of bone resorption. Amylin (Islet/insulinoma amyloid polypeptide or IAPP) is a 37-amino acid monomeric polypeptide isolated from pancreatic amyloid. This protein, with a calculated molecular mass of 4 kDa, is a major component of amyloid-rich pancreatic extracts of three type 2 diabetic patients. IAPP has cysteine residues in positions 2 and 7, a feature found in all known calcitonin gene-related peptides. IAPP shows 46% amino acid sequence homology with CGRP II. Since IAPP has been demonstrated immunochemically in normal beta cells of several mammals, it probably has an important role in respect to pancreatic islet function. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
Amylin (H28) polyclonal antibody detects endogenous levels of Amylin protein. Immunogen :
Synthetic peptide, corresponding to amino acids 1-50 of Human Amylin. Conjugate :
Unconjugated Modification :
Unmodification

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  Immunohistochemistry (IHC) analyzes of Amylin (H28) pAb in paraffin-embedded mouse brain tissue at 1:50,showing distinct cytoplasmic membrane and secreted staining.Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.
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  Immunohistochemistry (IHC) analyzes of Amylin (H28) pAb in paraffin-embedded mouse brain tissue at 1:50,showing distinct cytoplasmic membrane and secreted staining.Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.

Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

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Price/Size :

USD 368/1mg/vial

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Tips: 

For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).

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Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

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Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

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Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 

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Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.

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