Product Name :
Rit1 (W204) polyclonal antibody Background :
ROC 1 and ROC 2 are the two highly conserved RING finger proteins that are homologous to APC 11, a subunit of the anaphase promoting complex(APC). ROC 1 and ROC 2 commonly interact with all cullins while APC 11 specifically interacts with APC 2, a cullin related APC subunit. ROC 1 and APC 11 immunocomplexes can catalyze isopeptide ligations to form polyubiquitin chains in an E1 and E2 dependent manner. ROC 1 is thought to play a unique role in the ubiquitination reaction by heterodimerizing with cullin 1 to catalyze ubiquitin polymerization. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
ROC1 (W204) polyclonal antibody detects endogenous levels of ROC1 protein. Immunogen :
Synthetic peptide, corresponding to amino acids 170-220 of Human Rit1. Conjugate :
Unconjugated Modification :
Unmodification

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  Western blot (WB) analysis of Rit1 (W204) polyclonal antibody at 1:500 dilution Lane1:HEK293T whole cell lysate Lane2:sp2/0 whole cell lysate Lane3:H9C2 whole cell lysate

Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

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Price/Size :

USD 368/1mg/vial

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Tips: 

For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).

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Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

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Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

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Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 

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Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.

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