PTEN (D375) polyclonal antibody 
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COA
MSDS
SHIP
Product Name :
PTEN (D375) polyclonal antibody Background :
As human tumors progress to advanced stages, one genetic alteration that occurs at high frequency is a loss of heterozygosity (LOH) at chromosome 10q23. Mapping of homozygous deletions on this chromosome led to the isolation of the PTEN gene, also designated MMAC1 (for mutated in multiple advanced cancers) and TEP1. This candidate tumor suppressor gene exhibits a high frequency of mutations in human glioblastomas and is also mutated in other cancers, including sporadic brain, breast, kidney and prostate cancers. PTEN has been associated with Cowden disease, an autosomal dominant cancer predisposition syndrome. The PTEN gene product is a putative protein tyrosine phosphatase that is localized to the cytoplasm, and it shares extensive homology with the cytoskeletal proteins tensin and auxilin. Gene transfer studies have indicated that the phosphatase domain of PTEN is essential for growth suppression of glioma cells. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
PTEN (D375) polyclonal antibody detects endogenous levels of PTEN protein. Immunogen :
Synthetic peptide, corresponding to amino acids 350-400 of Human PTEN. Conjugate :
Unconjugated Modification :
Unmodification
PTEN (D375) polyclonal antibody Background :
As human tumors progress to advanced stages, one genetic alteration that occurs at high frequency is a loss of heterozygosity (LOH) at chromosome 10q23. Mapping of homozygous deletions on this chromosome led to the isolation of the PTEN gene, also designated MMAC1 (for mutated in multiple advanced cancers) and TEP1. This candidate tumor suppressor gene exhibits a high frequency of mutations in human glioblastomas and is also mutated in other cancers, including sporadic brain, breast, kidney and prostate cancers. PTEN has been associated with Cowden disease, an autosomal dominant cancer predisposition syndrome. The PTEN gene product is a putative protein tyrosine phosphatase that is localized to the cytoplasm, and it shares extensive homology with the cytoskeletal proteins tensin and auxilin. Gene transfer studies have indicated that the phosphatase domain of PTEN is essential for growth suppression of glioma cells. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
PTEN (D375) polyclonal antibody detects endogenous levels of PTEN protein. Immunogen :
Synthetic peptide, corresponding to amino acids 350-400 of Human PTEN. Conjugate :
Unconjugated Modification :
Unmodification
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Western blot (WB) analysis of PTEN (D375) polyclonal antibody at 1:500 dillution Lane1:HeLa whole cell lysate(50μg) Lane2:PC12 whole cell lysate(50μg) Lane3:RAW264.7 whole cell lysate(50μg) -
Immunohistochemistry (IHC) analyzes of PTEN (D375) pAb in paraffin-embedded human breast carcinoma tissue.
Expression patterns of USP22 and potential targets BMI-1, PTEN, p-AKT in non-small-cell lung cancer
PMCID: Pubmed No.:22717106
Anti-apoptotic Effect of MicroRNA-21 after Contusion Spinal Cord Injury in Rats
PMCID: Pubmed No.:23647386
miR-155* mediates suppressive effect of PTEN 3'-untranslated region on AP-1/NF-κB pathway in HTR-8/SVneo cells.
PMCID: Pubmed No.:23684381
Promotion of Melanoma Cell Invasion and Tumor Metastasis by Microcystin-LR via Phosphatidylinositol 3-Kinase/AKT Pathway.
PMCID: Pubmed No.:23802678
Hypoxia-Inducible Factor-1α Dependent Pathways Mediate the Renoprotective Role of Acetazolamide Against Renal Ischemia-Reperfusion Injury
PMCID: Pubmed No.:None
Hypoxia-Inducible Factor-1α Dependent Pathways Mediate the Renoprotective Role of Acetazolamide Against Renal Ischemia-Reperfusion Injury
PMCID: Pubmed No.:24335166
Paeonol protects rat vascular endothelial cells from ox-LDL-induced injury in vitro via downregulating microRNA-21 expression and TNF-α release
PMCID: Pubmed No.:24562307
MiR-205 inhibits cell apoptosis by targeting phosphatase and tensin homolog deleted on chromosome ten in endometrial cancer ishikawa cells
PMCID: Pubmed No.:24929707
PTEN inhibits macrophage polarization from M1 to M2 through CCL2 and VEGF-A reduction and NHERF-1 synergism
PMCID: Pubmed No.:25756512
MicroRNA-222 Controls Human Pancreatic Cancer Cell Line Capan-2 Proliferation by P57 Targeting
PMCID: Pubmed No.:26535064
miR-141 is involved in BRD7-mediated cell proliferation and tumor formation through suppression of the PTEN/AKT pathway in nasopharyngeal carcinoma
PMCID: Pubmed No.:27010857
miR-141 is involved in BRD7-mediated cell proliferation and tumor formation through suppression of the PTEN/ AKT pathway in nasopharyngeal carcinoma
PMCID: Pubmed No.:27010857
Hypoxia-Inducible Factor-1α Dependent Pathways Mediate the Renoprotective Role of Acetazolamide Against Renal Ischemia-Reperfusion Injury
PMCID: Pubmed No.:None
MicroRNA-222 Controls Human Pancreatic Cancer Cell Line Capan-2 Proliferation by P57 Targeting
PMCID: Pubmed No.:26535064
miR-141 is involved in BRD7-mediated cell proliferation and tumor formation through suppression of the PTEN/AKT pathway in nasopharyngeal carcinoma
PMCID: Pubmed No.:27010857
Hypoxia-Inducible Factor-1α Dependent Pathways Mediate the Renoprotective Role of Acetazolamide Against Renal Ischemia-Reperfusion Injury
PMCID: Pubmed No.:24335166
Paeonol protects rat vascular endothelial cells from ox-LDL-induced injury in vitro via downregulating microRNA-21 expression and TNF-α release
PMCID: Pubmed No.:24562307
Follicle loss and PTEN/PI3K/mTOR signaling pathway activated in LepR-mutated mice
PMCID: Pubmed No.:30145913
Mesenchymal stem cells-derived small extracellular vesicles alleviate diabetic retinopathy by delivering NEDD4
PMCID: Pubmed No.:35841055
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.
Price/Size :
USD 368/1mg/vial
Tips:
For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).Describe :
Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.Formula:
Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.
Storage:
The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C.
Note :
This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.