PML (P39) polyclonal antibody 
Datasheet
COA
MSDS
SHIP
Product Name :
PML (P39) polyclonal antibody Background :
The PML protein is a zinc finger transcription factor expressed as three major transcription products due to alternative splicing. The gene encoding human PML maps to chromosome 15q22. The t(15;17) (q22;q11.2-q12) chromosomal trans-location of the retinoic acid receptor α (RARα) gene occurs in virtually all cases of acute promyelocytic leukemia and results in the expression of a PML/RARα chimeric protein. Myeloid precursor cells expressing the PML/ RARα chimera fail to differentiate and exhibit an increased growth rate consequent to diminished apoptosis. PML/RARα transforms myeloid precursors by recruiting the nuclear co-repressor (N-CoR)-histone deacetylase complex that is essential to retinoic acid-dependent myeloid differentiation. PML/ RARα also recruits DNA methyltransferases in order to induce gene hypermethylation and silencing, which ultimately facilitates leukemogenesis. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
PML (P39) polyclonal antibody detects endogenous levels of PML protein. Immunogen :
Synthetic peptide, corresponding to the N-terminal of Human PML. Conjugate :
Unconjugated Modification :
Unmodification
PML (P39) polyclonal antibody Background :
The PML protein is a zinc finger transcription factor expressed as three major transcription products due to alternative splicing. The gene encoding human PML maps to chromosome 15q22. The t(15;17) (q22;q11.2-q12) chromosomal trans-location of the retinoic acid receptor α (RARα) gene occurs in virtually all cases of acute promyelocytic leukemia and results in the expression of a PML/RARα chimeric protein. Myeloid precursor cells expressing the PML/ RARα chimera fail to differentiate and exhibit an increased growth rate consequent to diminished apoptosis. PML/RARα transforms myeloid precursors by recruiting the nuclear co-repressor (N-CoR)-histone deacetylase complex that is essential to retinoic acid-dependent myeloid differentiation. PML/ RARα also recruits DNA methyltransferases in order to induce gene hypermethylation and silencing, which ultimately facilitates leukemogenesis. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
PML (P39) polyclonal antibody detects endogenous levels of PML protein. Immunogen :
Synthetic peptide, corresponding to the N-terminal of Human PML. Conjugate :
Unconjugated Modification :
Unmodification
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Western blot (WB) analysis of PML (P39) pAb at 1:500 dilution Lane1:L02 whole cell lysate(40ug) Lane2:HEK293T whole cell lysate(40ug) Lane3:A549 whole cell lysate(40ug) -
Immunohistochemistry (IHC) analyzes of PML (P39) pAb in paraffin-embedded human tonsil carcinoma tissue at 1:50.
Suppression of RAD21 induces senescence of MDA-MB-231 human breast cancer cells through RB1 pathway activation via c-Myc downregulation.
PMCID: Pubmed No.:26529363
Suppression of RAD21 induces senescence of MDA-MB-231 human breast cancer cells through RB1 pathway activation via c-Myc downregulation.
PMCID: Pubmed No.:26529363
Suppression of RAD21 Induces Senescence of MDA‐MB‐231 Human Breast Cancer Cells Through RB1 Pathway Activation Via c‐Myc Downregulation
PMCID: Pubmed No.:26529363
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.
Price/Size :
USD 368/1mg/vial
Tips:
For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).Describe :
Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.Formula:
Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.
Storage:
The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C.
Note :
This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.