Product Name :
p18 INK4c (D142) polyclonal antibody Background :
The normal progression of cells through the cell cycle is under the control of the cyclin dependent protein kinases Cdk4 and Cdk6 which are subject to inhibition by the mitotic inhibitory protein p16. Isolated members of the p16 family have been designated p15 and p18, respectively. The p16 related protein, p18, interacts strongly with Cdk6 and to a lesser extent with Cdk4, but lacks apparent interaction with other Cdks. Recombinant p18 has been shown to inhibit cyclin D-Cdk6 kinase activity. In contrast to p21/p27 that form ternary complexes with cyclin-Cdks, only binary complexes of p15, p16 and p18 have been identified in association with Cdk4 and/or Cdk6. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
p18 INK4c (D142) polyclonal antibody detects endogenous levels of p18 INK4c protein. Immunogen :
Synthetic peptide, corresponding to amino acids 100-150 of Human p18 INK4c. Conjugate :
Unconjugated Modification :
Unmodification

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  Western blot (WB) analysis of p18 INK4c (D142) polyclonal antibody at 1:500 dilution Lane1:MCF-7 whole cell lysate(40ug) Lane2:A549 whole cell lysate(40ug) Lane3:Hela whole cell lysate(40ug) Lane4:CT26 whole cell lysate(40ug) Lane5:PC12 whole cell lysate(40ug)
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  Immunohistochemistry (IHC) analysis of p18 INK4c (D142) pAb in paraffin-embedded human breast carcinoma tissue
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  Immunohistochemistry (IHC) analysis of p18 INK4c (D142) pAb in paraffin-embedded human breast carcinoma tissue

Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

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Price/Size :

USD 368/1mg/vial

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Tips: 

For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).

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Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

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Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

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Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 

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Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.

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