OPN (L285) polyclonal antibody 
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MSDS
SHIP
Product Name :
OPN (L285) polyclonal antibody Background :
Osteopontin (OPN), also designated bone sialoprotein 1, urinary stone protein, spp-1, Eta-1, nephropontin and uropontin, is an extracellular matrix cell adhesion phosphoglycoprotein. OPN is deposited into unmineralized matrix prior to calcification leading to localization at various tissue interfaces including cement lines, lamina limitans and between collagen fibrils of fully matured hard tissues. While OPN is a major product of osteoblasts, it is also synthesized by brain and kidney cells. OPN isolated from or secreted by various tissues ranges in molecular weight due to posttranslational modifications. OPN functions as a substrate for transglutaminase and is involved in cell adhesion, chemoattraction and immunomodulation. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
OPN (L285) polyclonal antibody detects endogenous levels of OPN protein. Immunogen :
Synthetic peptide, corresponding to amino acids 251-300 of Human OPN. Conjugate :
Unconjugated Modification :
Unmodification
OPN (L285) polyclonal antibody Background :
Osteopontin (OPN), also designated bone sialoprotein 1, urinary stone protein, spp-1, Eta-1, nephropontin and uropontin, is an extracellular matrix cell adhesion phosphoglycoprotein. OPN is deposited into unmineralized matrix prior to calcification leading to localization at various tissue interfaces including cement lines, lamina limitans and between collagen fibrils of fully matured hard tissues. While OPN is a major product of osteoblasts, it is also synthesized by brain and kidney cells. OPN isolated from or secreted by various tissues ranges in molecular weight due to posttranslational modifications. OPN functions as a substrate for transglutaminase and is involved in cell adhesion, chemoattraction and immunomodulation. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
OPN (L285) polyclonal antibody detects endogenous levels of OPN protein. Immunogen :
Synthetic peptide, corresponding to amino acids 251-300 of Human OPN. Conjugate :
Unconjugated Modification :
Unmodification
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Western blot (WB) analysis of OPN (L285) polyclonal antibody at 1:500 dilution Lane1:C6 whole cell lysate(40ug) Lane2:AML-12 whole cell lysate(40ug) Lane3:L02 whole cell lysate(40ug) Lane4:A549 whole cell lysate(40ug)
The E3 ubiquitin protein ligase MDM2 dictates all-trans retinoic acid-induced osteoblastic differentiation of osteosarcoma cells by modulating the degradation of RARα
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The E3 ubiquitin protein ligase MDM2 dictates all-trans retinoic acid-induced osteoblastic differentiation of osteosarcoma cells by modulating the degradation of RARα
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Pioglitazone attenuates progression of aortic valve calcification via down-regulating receptor for advanced glycation end products.
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Adrenomedullin Attenuates Vascular Calcification in Fructose-induced Insulin Resistance Rats
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The MEK5/ERK5 Pathway Mediates Fluid Shear Stress Promoted Osteoblast Differentiation
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Interferon Regulatory Factor 8 Modulates Phenotypic Switching of Smooth Muscle Cells through Regulating the Activity of Myocardin
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BMP-2 overexpression augments vascular smooth muscle cell motility by upregulating myosin Va via Erk signaling
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Phenotypic characterization of craniofacial bone marrow stromal cells: unique properties of enhanced osteogenesis, cell recruitment, autophagy, and apoptosis resistance.
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Interferon regulatory factor 9 is critical for neointima formation following vascular injury. (Application: Human WB, IF)
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Mangiferin Attenuates Renal Fibrosis Through Down‐Regulation of Osteopontin in Diabetic Rats
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The Imperatorin Derivative OW1, A new vasoactive compound, Inhibits VSMC proliferation and extracellular matrix hyperplasia
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Photoactivation of TAZ via Akt/GSK3β signaling pathway promotes osteogenic differentiation
PMCID: Pubmed No.:26159930
Advanced glycation end products accelerate arteriosclerosis after renal transplantation through the AGE/RAGE/ILK pathway
PMCID: Pubmed No.:26210487
Interferon regulatory factor 9 is critical for neointima formation following vascular injury. (Application: Human WB, IF)
PMCID: Pubmed No.:25319116
Mangiferin Attenuates Renal Fibrosis Through Down‐Regulation of Osteopontin in Diabetic Rats
PMCID: Pubmed No.:25380391
The MEK5/ERK5 Pathway Mediates Fluid Shear Stress Promoted Osteoblast Differentiation
PMCID: Pubmed No.:24111522
Interferon Regulatory Factor 8 Modulates Phenotypic Switching of Smooth Muscle Cells through Regulating the Activity of Myocardin
PMCID: Pubmed No.:24248596
BMP-2 overexpression augments vascular smooth muscle cell motility by upregulating myosin Va via Erk signaling
PMCID: Pubmed No.:24790701
Mechanisms of aortic dissection smooth muscle cell phenotype switch
PMCID: Pubmed No.:28625769
Mangiferin Attenuates Renal Fibrosis Through Down‐Regulation of Osteopontin in Diabetic Rats
PMCID: Pubmed No.:25380391
In Situ Release of VEGF Enhances Osteogenesis in 3D Porous Scaffolds Engineered with Osterix-Modified Adipose-Derived Stem Cells
PMCID: Pubmed No.:28107808
The imperatorin derivative OW1, a new vasoactive compound, inhibits VSMC proliferation and extracellular matrix hyperplasia
PMCID: Pubmed No.:25727310
T3 inhibits the calcification of vascular smooth muscle cells and the potential mechanism
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Photoactivation of TAZ via Akt/GSK3β signaling pathway promotes osteogenic differentiation
PMCID: Pubmed No.:26159930
Advanced glycation end products accelerate arteriosclerosis after renal transplantation through the AGE/RAGE/ILK pathway
PMCID: Pubmed No.:26210487
Silencing of Long Non-Coding RNA NONHSAT009968 Ameliorates the Staphylococcal Protein A-Inhibited Osteogenic Differentiation in Human Bone Mesenchymal Stem Cells
PMCID: Pubmed No.:27607236
Peroxisome Proliferator-Activated Receptor-γ Coactivator-1α Inhibits Vascular Calcification Through Sirtuin 3-Mediated Reduction of Mitochondrial Oxidative Stress
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Hydroxysafflor yellow A promotes osteogenesis and bone development via epigenetically regulating β-catenin and prevents ovariectomy-induced bone loss
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Inhibition of acetylation of histones 3 and 4 attenuates aortic valve calcification
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MiR155 modulates vascular calcification by regulating Akt‐FOXO3a signalling and apoptosis in vascular smooth muscle cells
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Celastrol attenuates arterial and valvular calcification via inhibiting BMP2/Smad1/5 signalling
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The role of Rho-GEF Trio in regulating tooth root development through the p38 MAPK pathway
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Osteopontin promotes cancer cell drug resistance, invasion, and lactate production and is associated with poor outcome of patients with advanced non-small-cell lung cancer
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Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.
Price/Size :
USD 368/1mg/vial
Tips:
For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).Describe :
Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.Formula:
Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.
Storage:
The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C.
Note :
This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.