Contact us : info@bioworlde.com
Home > Product > Primary Antibodies
NFkB-p65 (L523) polyclonal antibody BS1256
  • Western blot (WB) analysis of NFκB-p65 (L523) pAb at 1:500 dilution Lane1:MCF-7 whole cell lysate(40ug) Lane2:A549 whole cell lysate(40ug) Lane3:A2780 whole cell lysate(40ug)
  • Immunohistochemistry (IHC) analyzes of NFkB-p65 (L523) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.
Product NameNFkB-p65 (L523) polyclonal antibody
Catalog No.BS1256
Swiss-ProtQ04206
Host Rabbit
ReactivityHuman
ApplicationsWB IHC
Application_allWB: 1:500~1:1000 IHC: 1:50~1:200
BiowMW~ 60, 75 kDa
Alternative Namep65; p65 NF kappaB; p65 NFkB; RELA; NFKB P65; Transcription Factor p65; Nuclear factor NF-kappa-B p65 subunit; NFkB-p65; NFKB3
Purification&PurityThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
ConjugateUnconjugated
ModificationUnmodification
Browse similar products>>
Size Price
50ul $158
100ul $275
Add to cart My orders
Product Name :
NFkB-p65 (L523) polyclonal antibody
Background :
p65 is a subunit of the nuclear factor kappa B. The transcription factor NFkB is widely recognized as a critical mediator of immune and inflammatory responses. In most cell types, NFkB is found in the cytoplasm where it is associated with an inhibitory prd in many tissues. A high proportion of spontaneous NIH/3T3 transformants overexpress c-Met and by transfection analysis the c-Met proto-oncogene has been shown to exhibittransforming activity.
Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity :
NFκB-p65 (L523) polyclonal antibody detects endogenous levels of NFκB-p65 protein.
Immunogen :
Synthetic peptide, corresponding to amino acids 500-550 of Human NFkB-p65.
Conjugate :
Unconjugated
Modification :
Unmodification
  • Western blot (WB) analysis of NFκB-p65 (L523) pAb at 1:500 dilution Lane1:MCF-7 whole cell lysate(40ug) Lane2:A549 whole cell lysate(40ug) Lane3:A2780 whole cell lysate(40ug)
  • Immunohistochemistry (IHC) analyzes of NFkB-p65 (L523) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.
Maltose-binding protein isolated from Escherichia coli induces Toll-like receptor 2-mediated viability in U937 cells PMCID:    Pubmed No.:21775279 BML-111, a lipoxin receptor agonist, protects haemorrhagic shock-induced acute lung injury in rats PMCID:    Pubmed No.:22245750 Impairment of cellular immunity is associated with overexpression of heat shock protein 70 in neonatal pigs with intrauterine growth retardation PMCID:    Pubmed No.:22270614 Modulatory effects of the acid polysaccharide fraction from one of anamorph of Cordyceps sinensis on Ana-1 cells PMCID:    Pubmed No.:22710292 Low expression of glucocorticoid receptor alpha isoform in adult immune thrombocytopenia correlates with glucocorticoid resistance PMCID:    Pubmed No.:23435844 Toxicological effects of cigarette smoke on Ana-1 macrophages in vitro PMCID:    Pubmed No.:23517752 HSCARG Inhibits NADPH Oxidase Activity through Regulation of the Expression of p47phox PMCID:    Pubmed No.:23527155 Oxidative Burst of Circulating Neutrophils Following Traumatic Brain Injury in Human PMCID:    Pubmed No.:23894384 IGF-1 gene-modified muscle-derived stem cells are resistant to oxidative stress via enhanced activation of IGF-1R/PI3K/AKT signaling and secretion of VEGF PMCID:    Pubmed No.:24126783 Interaction between cyclooxygenase-2, Snail, and E-cadherin in gastric cancer cells PMCID:    Pubmed No.:24115825 FOXP3 inhibits NF-κB activity and hence COX2 expression in gastric cancer cells PMCID:    Pubmed No.:24308961 Adenosine 5′-monophosphate ameliorates d-galactosamine/lipopolysaccharide-induced liver injury through an adenosine receptor-independent mechanism in mice PMCID:    Pubmed No.:24407238 Poloxamer-188 Can Attenuate Blood–Brain Barrier Damage to Exert Neuroprotective Effect in Mice Intracerebral Hemorrhage Model PMCID:    Pubmed No.:24770901 Activation of Mesenchymal Stem Cells by Macrophages Prompts Human Gastric Cancer Growth through NF-κB Pathway PMCID:    Pubmed No.:24824968 Protective effects of bifidobacterial adhesin on intestinal mucosa of stressed male rats via modulation of inflammation PMCID:    Pubmed No.:25031756 Effects of Cichoric Acid Extract from Echinacea purpurea on Collagen-Induced Arthritis in Rats PMCID:    Pubmed No.:24871659 Effect of glutamine on intestinal barrier function following liver transplantation in rats PMCID:    Pubmed No.:21813386 Mitofusin 2 deficiency leads to oxidative stress that contributes to insulin resistance in rat skeletal muscle cells PMCID:    Pubmed No.:25034891 Penehyclidine hydrochloride postconditioning on lipopolysaccharide-induced acute lung injury by inhibition of inflammatory factors in a rodent model PMCID:    Pubmed No.:25539663 Regional Arterial Infusion with Lipoxin A4 Attenuates Experimental Severe Acute Pancreatitis PMCID:    Pubmed No.:25265022 Arctigenin but not arctiin acts as the major effective constituent of Arctium lappa L. fruit for attenuating colonic inflammatory response induced by dextran sulfate sodium in mice. (Application: Mouse WB) PMCID:    Pubmed No.:25284342 Mangiferin Attenuates Renal Fibrosis Through Down‐Regulation of Osteopontin in Diabetic Rats PMCID:    Pubmed No.:25380391 Noscapine Increases the Sensitivity of Drug-Resistant Ovarian Cancer Cell Line SKOV3/DDP to Cisplatin by Regulating Cell Cycle and Activating Apoptotic Pathways PMCID:    Pubmed No.:25510462 Alpha-Lipoic Acid Promotes Osteoblastic Formation in H2 O2 -Treated MC3T3-E1 Cells and Prevents Bone Loss in Ovariectomized Rats. PMCID:    Pubmed No.:25655087 Pyruvate kinase M2 accelerates pro-inflammatory cytokine secretion and cell proliferation induced by lipopolysaccharide in colorectal cancer PMCID:    Pubmed No.:25778902 Inhibitory effects of Chikusetsusaponin IVa on lipopolysaccharide-induced pro-inflammatory responses in THP-1 cells. PMCID:    Pubmed No.:26157065 Penehyclidine hydrochloride postconditioning on lipopolysaccharide-induced acute lung injury by inhibition of inflammatory factors in a rodent model PMCID:    Pubmed No.:25539663 Regional Arterial Infusion with Lipoxin A4 Attenuates Experimental Severe Acute Pancreatitis PMCID:    Pubmed No.:25265022 Arctigenin but not arctiin acts as the major effective constituent of Arctium lappa L. fruit for attenuating colonic inflammatory response induced by dextran sulfate sodium in mice. (Application: Mouse WB) PMCID:    Pubmed No.:25284342 Mangiferin Attenuates Renal Fibrosis Through Down‐Regulation of Osteopontin in Diabetic Rats PMCID:    Pubmed No.:25380391 Noscapine Increases the Sensitivity of Drug-Resistant Ovarian Cancer Cell Line SKOV3/DDP to Cisplatin by Regulating Cell Cycle and Activating Apoptotic Pathways PMCID:    Pubmed No.:25510462 IGF-1 gene-modified muscle-derived stem cells are resistant to oxidative stress via enhanced activation of IGF-1R/PI3K/AKT signaling and secretion of VEGF PMCID:    Pubmed No.:24126783 Interaction between cyclooxygenase-2, Snail, and E-cadherin in gastric cancer cells PMCID:    Pubmed No.:24115825 Poloxamer-188 Can Attenuate Blood–Brain Barrier Damage to Exert Neuroprotective Effect in Mice Intracerebral Hemorrhage Model PMCID:    Pubmed No.:24770901 Activation of Mesenchymal Stem Cells by Macrophages Prompts Human Gastric Cancer Growth through NF-κB Pathway PMCID:    Pubmed No.:24824968 FOXP3 inhibits NF-κB activity and hence COX2 expression in gastric cancer cells PMCID:    Pubmed No.:24308961 Adenosine 5′-monophosphate ameliorates d-galactosamine/lipopolysaccharide-induced liver injury through an adenosine receptor-independent mechanism in mice PMCID:    Pubmed No.:24407238 A comparison of the impact of Shimen and C strains of classical swine fever virus on Toll-like receptor expression PMCID:    Pubmed No.:25805409 The combination effects of Shen-Ling-Bai-Zhu on promoting apoptosis of transplanted H22 hepatocellular carcinoma in mice receiving chemotherapy PMCID:    Pubmed No.:27235019
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

Price/Size :

USD 368/1mg/vial



Tips: 

For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).

Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 


Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.
COPYRIGHT © 2015-2018 Bioworld Technology, Inc. All rights reserved POLYCLONAL AND MONOCLONAL ANTIBODY CENTER