Product Name :
NFkB-p105/p50 (Q332) polyclonal antibody Background :
Proteins encoded by the v-Rel viral oncogene and its cellular homolog, c-Rel, are members of a family of transcription factors that include the two subunits of the transcription factor NFκB (p50 and p65) and the Drosophila maternal morphogen, dorsal. The DNA binding activity of NFκB is activated and NFκB is subsequently transported from the cytoplasm to the nucleus in cells exposed to mitogens or growth factors. cDNAs encoding precursors for two distinct proteins of the same size have been described, designated p105 and p100. The p105 precursor contains p50 at its N-terminus and a C-terminal region that when expressed as a separate molecule, designated pdI, binds to p50 and regulates its activity Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
NFkB-p105/p50 (Q332) polyclonal antibody detects endogenous levels of the precurser protein p105 and its cleavage product p50. Immunogen :
Synthetic peptide, corresponding to amino acids 300-350 of Human NFkB-p105. Conjugate :
Unconjugated Modification :
Unmodification
NFkB-p105/p50 (Q332) polyclonal antibody Background :
Proteins encoded by the v-Rel viral oncogene and its cellular homolog, c-Rel, are members of a family of transcription factors that include the two subunits of the transcription factor NFκB (p50 and p65) and the Drosophila maternal morphogen, dorsal. The DNA binding activity of NFκB is activated and NFκB is subsequently transported from the cytoplasm to the nucleus in cells exposed to mitogens or growth factors. cDNAs encoding precursors for two distinct proteins of the same size have been described, designated p105 and p100. The p105 precursor contains p50 at its N-terminus and a C-terminal region that when expressed as a separate molecule, designated pdI, binds to p50 and regulates its activity Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
NFkB-p105/p50 (Q332) polyclonal antibody detects endogenous levels of the precurser protein p105 and its cleavage product p50. Immunogen :
Synthetic peptide, corresponding to amino acids 300-350 of Human NFkB-p105. Conjugate :
Unconjugated Modification :
Unmodification
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Western blot (WB) analysis of NFkB-p105/p50 (Q332) polyclonal antibody at 1:1000 dilution Lane1:HEK293T whole cell lysate(45ug) Lane2:RAW264.7 whole cell lysate(46ug) Lane3:H9C2 whole cell lysate(37ug) Lane4:HepG2 whole cell lysate(46ug)
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Immunohistochemistry (IHC) analyzes of NFkB-p105/p50 (Q332) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.
Regulation of the exopolysaccharide from an anamorph of Cordyceps sinensis on dendritic cell sarcoma (DCS) cell line
PMCID: Pubmed No.:22610670
Diosgenin down-regulates NF-κB p65/p50 and p38MAPK pathways and attenuates acute lung injury induced by lipopolysaccharide in mice
PMCID: Pubmed No.:23246979
Toxicological effects of cigarette smoke on Ana-1 macrophages in vitro
PMCID: Pubmed No.:23517752
Ozone Therapy Attenuates NF-κB-Mediated Local Inflammatory Response and Activation of Th17 Cells in Treatment for Psoriasis
PMCID: Pubmed No.:32398953
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.
Price/Size :
USD 368/1mg/vial
Tips:
For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).Describe :
Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.Formula:
Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.
Storage:
The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C.
Note :
This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.