Product Name :
MCM2 / BM28 (A11) polyclonal antibody Background :
The mini-chromosome maintenance (MCM) family of proteins, including MCM2, MCM3, MCM4 (Cdc21), MCM5 (Cdc46), MCM6 (Mis5) and MCM7 (Cdc47), are regulators of DNA replication that act to ensure replication occurs only once in the cell cycle. Expression of MCM proteins increases during cell growth, peaking at G1 to S phase. The MCM proteins each contain an ATPbinding motif, which is predicted to mediate ATP-dependent opening of doublestranded DNA. MCM proteins are regulated by E2F transcription factors, which induce MCM expression, and by protein kinases, which interact with MCM proteins to maintain the ostreplicative state of the cell. MCM2/MCM4 complexes function as substrates for Cdc2/cyclin B in vitro. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
MCM2 (A11) polyclonal antibody detects endogenous levels of MCM2 protein. Immunogen :
Synthetic peptide, corresponding to amino acids N-terminus of Human MCM2. Conjugate :
Unconjugated Modification :
Unmodification

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  Western blot (WB) analysis of BM28 / MCM2 (A11) pAb at 1:500 dilution Lane1:SK-OVCAR3 whole cell lysate(40ug) Lane2:HCT116 whole cell lysate(40ug) Lane3:CT26 whole cell lysate(40ug) Lane4:The Testis tissue lysate of Rat(40ug)
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  Immunohistochemistry (IHC) analyzes of MCM2 / BM28 (A11) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.
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  Immunohistochemistry (IHC) analyzes of MCM2 / BM28 (A11) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.

Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

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Price/Size :

USD 368/1mg/vial

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Tips: 

For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).

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Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

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Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

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Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 

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Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.

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