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NOS2 (V1131) polyclonal antibody BS1186
  • Western blot (WB) analysis of NOS2 (V1131) polyclonal antibody at 1:500 dilution Lane1:MCF-7 cell lysate Lane2:SP2/0 cell lysate Lane3:PC12 cell lysate Lane4:Rat liver tissue lysate
  • Immunohistochemistry (IHC) analyzes of NOS2 (V1131) pAb in paraffin-embedded human lung carcinoma tissue.
Product NameNOS2 (V1131) polyclonal antibody
Catalog No.BS1186
Swiss-ProtP35228
Host Rabbit
ReactivityHuman,Mouse,Rat
ApplicationsWB IHC IF
Application_allWB: 1:500~1:1000 IHC: 1:50~1:200 IF: 1:50~1:200
BiowMW~ 130 kDa
Alternative NameNitric oxide synthase, inducible; Hepatocyte NOS; HEP-NOS; Inducible NO synthase; Inducible NOS; Short name=iNOS; NOS type II; Peptidyl-cysteine S-nitrosylase; NOS 2; NOS2A; Inos
Purification&PurityThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
ConjugateUnconjugated
ModificationUnmodification
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Size Price
50ul $158
100ul $275
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Product Name :
NOS2 (V1131) polyclonal antibody
Background :
Several distinct NOS isoforms have been described and been shown to represent the products of three distinct genes. These include two constitutive Ca2+/CaM-dependent forms of NOS, including NOS1 (also designated ncNOS) whose activity was first identified in neurons, and NOS3 (also designated ecNOS), first identified in endothelial cells. The inducible form of NOS, NOS2 (also designated iNOS), is Ca2+-independent and is expressed in a broad range of cell types.
Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity :
NOS2 (V1131) polyclonal antibody detects endogenous levels of Inducible NOS protein.
Immunogen :
Synthetic peptide, corresponding to amino acids C-terminus of Human NOS2.
Conjugate :
Unconjugated
Modification :
Unmodification
  • Western blot (WB) analysis of NOS2 (V1131) polyclonal antibody at 1:500 dilution Lane1:MCF-7 cell lysate Lane2:SP2/0 cell lysate Lane3:PC12 cell lysate Lane4:Rat liver tissue lysate
  • Immunohistochemistry (IHC) analyzes of NOS2 (V1131) pAb in paraffin-embedded human lung carcinoma tissue.
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Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

Price/Size :

USD 368/1mg/vial



Tips: 

For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).

Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 


Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.
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