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GRP78 (P641) polyclonal antibody BS1154
  • Western blot (WB) analysis of GRP78 (P641) polyclonal antibody at 1:500 dilution Lane1:L02 whole cell lysate(40ug) Lane2:HCT116 whole cell lysate(40ug)Lane3:HCT116 whole cell lysate(40ug) Lane3:AML-12 whole cell lysate(40ug) Lane4:PC12 whole cell lysate(40ug)Lane3:HCT116 whole cell lysate(40ug) Lane5:HEK293T whole cell lysate(40ug)
  • Immunohistochemistry (IHC) analyzes of GRP78 (P641) pAbb in paraffin-embedded human breast carcinoma tissue at 1:100.
  • Immunohistochemistry (IHC) analyzes of GRP78 (P641) pAbb in paraffin-embedded human breast carcinoma tissue at 1:100.
Product NameGRP78 (P641) polyclonal antibody
Catalog No.BS1154
Swiss-ProtP11021
Host Rabbit
ReactivityHuman,Mouse,Rat
ApplicationsWB IHC IF IP
Application_allWB: 1:500~1:1000 IHC/IF: 1:50~1:200 IP: 1:10~1:100
BiowMW~ 78 kDa
Alternative Name78 kDa glucose-regulated protein; GRP-78; Endoplasmic reticulum lumenal Ca(2+)-binding protein grp78; Heat shock 70 kDa protein 5; Immunoglobulin heavy chain-binding protein; BiP; HSPA5; GRP78
Purification&PurityThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
ConjugateUnconjugated
ModificationUnmodification
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Size Price
50ul $158
100ul $275
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Product Name :
GRP78 (P641) polyclonal antibody
Background :
The HSP 70 family comprises four highly conserved proteins, HSP 70, HSC 70, GRP 75 and GRP 78, which serve a variety of roles. They act as molecular chaperones facilitating the assembly of multi-protein complexes, participate in the translocation of polypeptides across cell membranes and to the nucleus, and aid in the proper folding of nascent polypeptide chains. HSC 70, GRP 75 and GRP 78 are constitutively expressed in primate cells. HSP 70 expression is strongly induced in response to heat stress. HSP 70 and HSC 70, which are found in both the cytosol and nucleus of mammalian cells, play key roles in the cytosolic endoplasmic reticulum and mitochondrial import machinery.
Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity :
GRP78 (P641) polyclonal antibody detects endogenous levels of GRP78 protein.
Immunogen :
Synthetic peptide, corresponding to amino acids 611-660 of Human GRP78.
Conjugate :
Unconjugated
Modification :
Unmodification
  • Western blot (WB) analysis of GRP78 (P641) polyclonal antibody at 1:500 dilution Lane1:L02 whole cell lysate(40ug) Lane2:HCT116 whole cell lysate(40ug)Lane3:HCT116 whole cell lysate(40ug) Lane3:AML-12 whole cell lysate(40ug) Lane4:PC12 whole cell lysate(40ug)Lane3:HCT116 whole cell lysate(40ug) Lane5:HEK293T whole cell lysate(40ug)
  • Immunohistochemistry (IHC) analyzes of GRP78 (P641) pAbb in paraffin-embedded human breast carcinoma tissue at 1:100.
  • Immunohistochemistry (IHC) analyzes of GRP78 (P641) pAbb in paraffin-embedded human breast carcinoma tissue at 1:100.
Association of Elevated GRP78 Expression with Increased Astrocytoma Malignancy via Akt and ERK Pathways PMCID:    Pubmed No.:21112319 Japanese encephalitis virus co-opts the ER-stress response protein GRP78 for viral infectivity PMCID:    Pubmed No.:21418596 The protease allergen Pen c 13 induce allergic airway inflammation and changes in epithelial barrier integrity and function in a murine model PMCID:    Pubmed No.:21613216 ROS-activated p38 MAPK/ERK-Akt cascade plays a central role in palmitic acid-stimulated hepatocyte proliferation. PMCID:    Pubmed No.:21620957 The Invasion of Tobacco Mosaic Virus RNA Induces Endoplasmic ndoplasmic Reticulum Stress Related Autophagy Autophagy Autophagy in HeLa cells PMCID:    Pubmed No.:21729006 The invasion of tobacco mosaic virus RNA induces endoplasmic reticulum stress-related autophagy in HeLa cells PMCID:    Pubmed No.:21729006 Hydrogen sulfide protects H9c2 cells against doxorubicin-induced cardiotoxicity through inhibition of endoplasmic reticulum stress PMCID:    Pubmed No.:22203419 Glucose oxidase induces insulin resistance via influencing multiple targets in vitro and in vivo: The central role of oxidative stress PMCID:    Pubmed No.:22522094 Activation of the unfolded protein response contributed to the selective cytotoxicity of oroxylin A in human hepatocellular carcinoma HepG2 cells PMCID:    Pubmed No.:22609744 Activated Protein C Induces Endoplasmic Reticulum Stress and Attenuates Lipopolysaccharide-Induced Apoptosis Mediated by Glycogen Synthase Kinase-3β PMCID:    Pubmed No.:22690042 Phycocyanin may suppress d-galactose-induced human lens epithelial cell apoptosis through mitochondrial and unfolded protein response pathways PMCID:    Pubmed No.:23036742 Transmembrane Protein 208: A Novel ER-Localized Protein That Regulates Autophagy and ER Stress. PMCID:    Pubmed No.:23691174 V8, a newly synthetic flavonoid, induces apoptosis through ROS-mediated ER stress pathway in hepatocellular carcinoma PMCID:    Pubmed No.:23835921 Differential proteome and gene expression reveal response to carbon ion irradiation in pubertal mice testes PMCID:    Pubmed No.:24440814 Molecular Mechanisms of Exopolysaccharide from Aphanothece halaphytica (EPSAH) Induced Apoptosis in HeLa Cells PMCID:    Pubmed No.:24466342 Baicalin Protects the Cardiomyocytes from ER Stress-Induced Apoptosis: Inhibition of CHOP through Induction of Endothelial Nitric Oxide Synthase PMCID:    Pubmed No.:24520378 Lack of seipin in neurons results in anxiety-and depression-like behaviors via down regulation of PPARγ PMCID:    Pubmed No.:24651066 ER stress and ER stress-induced apoptosis are activated in gastric SMCs in diabetic rats PMCID:    Pubmed No.:25009401 Really interesting new gene finger protein 121 is a novel Golgi-localized membrane protein that regulates apoptosis PMCID:    Pubmed No.:24928685 Changes in the proteomic profile during the differential polarization status of the human monocyte‐derived macrophage THP‐1 cell line PMCID:    Pubmed No.:25411139 Activation of volume-sensitive outwardly rectifying chloride channel by ROS contributes to ER stress and cardiac contractile dysfunction: involvement of CHOP through Wnt. PMCID:    Pubmed No.:25412307 Endoplasmic Reticulum Stress May Not Be Involved in Intestinal Epithelial Cell Apoptosis in Experimental Acute Pancreatitis PMCID:    Pubmed No.:25616612 The p-eIF2α/ATF4 pathway links endoplasmic reticulum stress to autophagy following the production of reactive oxygen species in mouse spermatocyte-derived cells exposed to Dibutyl phthalate. PMCID:    Pubmed No.:27002192 E platinum, a newly synthesized platinum compound, induces apoptosis through ROS‐triggered ER stress in gastric carcinoma cells PMCID:    Pubmed No.:27061377 Deficiency of CC chemokine ligand 2 and decay-accelerating factor causes retinal degeneration in mice PMCID:    Pubmed No.:26149093 Changes in the proteomic profile during the differential polarization status of the human monocyte‐derived macrophage THP‐1 cell line PMCID:    Pubmed No.:25411139 Activation of volume-sensitive outwardly rectifying chloride channel by ROS contributes to ER stress and cardiac contractile dysfunction: involvement of CHOP through Wnt. PMCID:    Pubmed No.:25412307 The p-eIF2α/ATF4 pathway links endoplasmic reticulum stress to autophagy following the production of reactive oxygen species in mouse spermatocyte-derived cells exposed to Dibutyl phthalate. PMCID:    Pubmed No.:27002192 E platinum, a newly synthesized platinum compound, induces apoptosis through ROS‐triggered ER stress in gastric carcinoma cells PMCID:    Pubmed No.:27061377 Differential proteome and gene expression reveal response to carbon ion irradiation in pubertal mice testes PMCID:    Pubmed No.:24440814 Molecular Mechanisms of Exopolysaccharide from Aphanothece halaphytica (EPSAH) Induced Apoptosis in HeLa Cells PMCID:    Pubmed No.:24466342 Baicalin Protects the Cardiomyocytes from ER Stress-Induced Apoptosis: Inhibition of CHOP through Induction of Endothelial Nitric Oxide Synthase PMCID:    Pubmed No.:24520378 Lack of seipin in neurons results in anxiety-and depression-like behaviors via down regulation of PPARγ PMCID:    Pubmed No.:24651066 Experimental study of the protective effects of SYVN1 against diabetic retinopathy PMCID:    Pubmed No.:26358086 A novel endoplasmic reticulum stress‑induced apoptosis model using tunicamycin in primary cultured neonatal rat cardiomyocytes PMCID:    Pubmed No.:26151415 The p-eIF2α/ATF4 pathway links endoplasmic reticulum stress to autophagy following the production of reactive oxygen species in mouse spermatocyte-derived cells exposed to dibutyl phthalate PMCID:    Pubmed No.:27002192 Seipin deficiency in mice causes loss of dopaminergic neurons via aggregation and phosphorylation of α-synuclein and neuroinflammation PMCID:    Pubmed No.:29670081 4-Phenylbutyric acid, a potent endoplasmic reticulum stress inhibitor, attenuates the severity of collagen-induced arthritis in mice via inhibition of proliferation and inflammatory responses of synovial fibroblasts PMCID:    Pubmed No.:33759334 Exosomes derived from endoplasmic reticulum‑stressed liver cancer cells enhance the expression of cytokines in macrophages via the STAT3 signaling pathway PMCID:    Pubmed No.:32565984 Rosuvastatin protects against endothelial cell apoptosis in vitro and alleviates atherosclerosis in ApoE‑/‑ mice by suppressing endoplasmic reticulum stress PMCID:    Pubmed No.:32537013 Therapeutic propensity of ginsenosides Rg1 and Rg3 in rhabdomyolysis-induced acute kidney injury and renohepatic crosstalk in rats PMCID:    Pubmed No.:36580761 Mycobacterium tuberculosis PE_PGRS1 promotes mycobacteria intracellular survival via reducing the concentration of intracellular free Ca2+ and suppressing endoplasmic reticulum stress PMCID:    Pubmed No.:36584479
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

Price/Size :

USD 368/1mg/vial



Tips: 

For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).

Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 


Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.
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