CHOP (K121) polyclonal antibody 
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Product Name :
CHOP (K121) polyclonal antibody Background :
GADD153 is a small nuclear protein that is capable of dimerizing with transcription factors C/EBP alpha and beta. Once dimerized, this complex inhibits the normal binding and function of C/EBP to classical binding sites. Inversely, the C/EBP GADD153 dimer gains binding activity to other non classical C/EBP stress related targets. Under normal cellular conditions this protein is not expressed in detectable levels, but is highly unregulated during times of cellular/ER stress. Examples of GADD153 inducing stress include: treatment with tunicamycin, nutrient starvation and reducing agents that interfere with the calcium flux across the ER membrane. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
CHOP (K121) polyclonal antibody detects endogenous levels of CHOP protein. Immunogen :
Synthetic peptide, corresponding to amino acids 100-150 of Human CHOP. Conjugate :
Unconjugated Modification :
Unmodification
CHOP (K121) polyclonal antibody Background :
GADD153 is a small nuclear protein that is capable of dimerizing with transcription factors C/EBP alpha and beta. Once dimerized, this complex inhibits the normal binding and function of C/EBP to classical binding sites. Inversely, the C/EBP GADD153 dimer gains binding activity to other non classical C/EBP stress related targets. Under normal cellular conditions this protein is not expressed in detectable levels, but is highly unregulated during times of cellular/ER stress. Examples of GADD153 inducing stress include: treatment with tunicamycin, nutrient starvation and reducing agents that interfere with the calcium flux across the ER membrane. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
CHOP (K121) polyclonal antibody detects endogenous levels of CHOP protein. Immunogen :
Synthetic peptide, corresponding to amino acids 100-150 of Human CHOP. Conjugate :
Unconjugated Modification :
Unmodification
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Western blot (WB) analysis of CHOP (K121) pAb at 1:500 dilution Lane1:A375 whole cell lysate(40ug) Lane2:HCT116 whole cell lysate(40ug) Lane3:The Kidney tissue lysate of Mouse(40ug) Lane4:PC12 whole cell lysate(40ug) -
Immunohistochemistry (IHC) analyzes of CHOP (K121) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.
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LZ-106, a novel analogue of enoxacin, inducing apoptosis via activation of ROS-dependent DNA damage response in NSCLCs
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E platinum, a newly synthesized platinum compound, induces apoptosis through ROS‐triggered ER stress in gastric carcinoma cells
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Reconstructed mung bean trypsin inhibitor targeting cell surface GRP78 induces apoptosis and inhibits tumor growth in colorectal cancer
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Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.
Price/Size :
USD 368/1mg/vial
Tips:
For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).Describe :
Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.Formula:
Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.
Storage:
The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C.
Note :
This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.