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E-Cadherin (R868) polyclonal antibody BS1098
  • Western blot (WB) analysis of E-cadherin (R868) polyclonal antibody at 1:500 dilution Lane1:HCT116 whole cell lysate(40ug) Lane2:AML-12 whole cell lysate(40ug) Lane3:PC12 whole cell lysate(40ug) Lane4:L02 whole cell lysate(40ug) Lane5:HEK293T whole cell lysate(40ug)
  • Immunohistochemistry (IHC) analyzes of E-Cadherin (R868) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.
  • Immunohistochemistry (IHC) analyzes of E-Cadherin (R868) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.
Product NameE-Cadherin (R868) polyclonal antibody
Catalog No.BS1098
Swiss-ProtP12830
Host Rabbit
ReactivityHuman,Mouse,Rat
ApplicationsWB IHC IF IP
Application_allWB: 1:500~1:1000 IHC/IF: 1:50~1:200 IP: 1:50~1:200
BiowMW~ 98, 130 kDa
Alternative NameCadherin-1; Cadherin1; Cadherin 1; CAM 120/80; Epithelial cadherin; E-cadherin; Uvomorulin; CD324; CDH1; CDHE; UVO
Purification&PurityThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
ConjugateUnconjugated
ModificationUnmodification
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Size Price
50ul $158
100ul $275
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Product Name :
E-Cadherin (R868) polyclonal antibody
Background :
Cadherins are members of a multigene family of single chain glycoprotein receptors mediating calcium dependent cell-cell adhesion. They play an important role in the growth and development of cells via the mechanisms of control of tissue architecture and the maintenance of tissue integrity. Cadherins are expressed in a tissue specific manner and and are required for assembly of cells into solid tissue. Individual cadherin molecules are known to co-operate with each other to form a linear cell adhesion zipper. In adhesion junctions cadherins are bound to beta and gamma catenins which in turn bind to alpha catenin, an actin binding protein. Cadherins play an important part in tumor invasion and metastasis.
Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity :
E-cadherin (R868) polyclonal antibody detects endogenous levels of E-cadherin protein.
Immunogen :
Synthetic peptide, corresponding to amino acids 840-880 of Human E-cadherin.
Conjugate :
Unconjugated
Modification :
Unmodification
  • Western blot (WB) analysis of E-cadherin (R868) polyclonal antibody at 1:500 dilution Lane1:HCT116 whole cell lysate(40ug) Lane2:AML-12 whole cell lysate(40ug) Lane3:PC12 whole cell lysate(40ug) Lane4:L02 whole cell lysate(40ug) Lane5:HEK293T whole cell lysate(40ug)
  • Immunohistochemistry (IHC) analyzes of E-Cadherin (R868) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.
  • Immunohistochemistry (IHC) analyzes of E-Cadherin (R868) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.
Hepatitis C virus core protein interacts with Snail and histone deacetylases to promote the metastasis of hepatocellular carcinoma PMCID:    Pubmed No.:26549030 Proton pump inhibitor pantoprazole abrogates adriamycin-resistant gastric cancer cell invasiveness via suppression of Akt/GSK-β/β-catenin signaling and epithelial–mesenchymal transition PMCID:    Pubmed No.:25449432 The influence of fasudil on the epithelial-mesenchymal transdifferentiation of renal tubular epithelial cells from diabetic rats PMCID:    Pubmed No.:20006464 Knockdown of ribonuclease inhibitor expression with siRNA in non-invasive bladder cancer cell line BIU-87 promotes growth and metastasis potentials PMCID:    Pubmed No.:21125316 Osteopontin deficiency protects against aldosterone-induced inflammation, oxidative stress, and interstitial fibrosis in the kidney. PMCID:    Pubmed No.:21734100 Up-regulating ribonuclease inhibitor inhibited epithelial-to-mesenchymal transition and metastasis in murine melanoma cells PMCID:    Pubmed No.:22465710 Downregulation of integrin-linked kinase inhibits epithelial-to-mesenchymal transition and metastasis in bladder cancer cells PMCID:    Pubmed No.:22570869 ERβ-Mediated Estradiol Enhances Epithelial Mesenchymal Transition of Lung Adenocarcinoma through Increasing Transcription of Midkine PMCID:    Pubmed No.:22669742 β-casomorphin-7 attenuates the development of nephropathy in type I diabetes via inhibition of epithelial-mesenchymal transition of renal tubular epithelial cells PMCID:    Pubmed No.:22687367 β-Casomorphin-7 attenuates the development of nephropathy in type I diabetes via inhibition of epithelial–mesenchymal transition of renal tubular epithelial cells PMCID:    Pubmed No.:22687367 Interleukin-22 Inhibits Bleomycin-Induced Pulmonary Fibrosis PMCID:    Pubmed No.:23476100 Livin promotes progression of breast cancer through induction of epithelial–mesenchymal transition and activation of AKT signaling PMCID:    Pubmed No.:23524337 Twist2 is a valuable prognostic biomarker for colorectal cancer PMCID:    Pubmed No.:23613636 MicroRNA-21 suppresses PTEN and hSulf-1 expression and promotes hepatocellular carcinoma progression through AKT/ERK pathways PMCID:    Pubmed No.:23684551 A novel role of ribonuclease inhibitor in regulation of epithelial-to-mesenchymal transition and ILK signaling pathway in bladder cancer cells PMCID:    Pubmed No.:23703635 Small interfering RNA targeting ILK inhibits metastasis in human tongue cancer cells through repression of epithelial-to-mesenchymal transition PMCID:    Pubmed No.:23707970 microRNA-200c modulates the epithelial-to-mesenchymal transition in human renal cell carcinoma metastasis. PMCID:    Pubmed No.:23754305 Interaction between cyclooxygenase-2, Snail, and E-cadherin in gastric cancer cells PMCID:    Pubmed No.:24115825 LncRNA expression signatures of twist-induced epithelial-to-mesenchymal transition in MCF10A cells PMCID:    Pubmed No.:24113349 H. pylori infection-induced MSC differentiation into CAFs promotes epithelial-mesenchymal transition in gastric epithelial cells PMCID:    Pubmed No.:24145921 Benidipine Protects Kidney through Inhibiting ROCK1 Activity and Reducing the Epithelium-Mesenchymal Transdifferentiation in Type 1 Diabetic Rats PMCID:    Pubmed No.:24364038 Epigenetic silencing of SFRP1 and SFRP5 by hepatitis B virus X protein enhances hepatoma cell tumorigenicity through Wnt signaling pathway PMCID:    Pubmed No.:24374650 Synergism from the combination of ulinastatin and curcumin offers greater inhibition against colorectal cancer liver metastases via modulating matrix metalloproteinase-9 and E-cadherin expression. PMCID:    Pubmed No.:24570592 TGFbetaR2 is a major target of miR-93 in nasopharyngeal carcinoma aggressiveness PMCID:    Pubmed No.:24606633 Regulation gene expression of miR200c and ZEB1 positively enhances effect of tumor vaccine B16F10/GPI-IL-21 on inhibition of melanoma growth and metastasis PMCID:    Pubmed No.:24625224 Oroxylin A inhibits hypoxia-induced invasion and migration of MCF-7 cells by suppressing the Notch pathway PMCID:    Pubmed No.:24637576 Evidence of TGF-β 1 mediated epithelial-mesenchymal transition in immortalized benign prostatic hyperplasia cells PMCID:    Pubmed No.:24650126 Id-1, a Protein Repressed by miR-29b, Facilitates the TGFβ1-Induced Epithelial-Mesenchymal Transition in Human Ovarian Cancer Cells PMCID:    Pubmed No.:24662327 Down-regulating ribonuclease inhibitor enhances metastasis of bladder cancer cells through regulating epithelial-mesenchymal transition and ILK signaling pathway PMCID:    Pubmed No.:24768914 PMCID:    Pubmed No.:24812621 Epigenetic silencing of DACH1 induces the invasion and metastasis of gastric cancer by activating TGF-β signalling. PMCID:    Pubmed No.:24912879 Neuropilin-1 Promotes Epithelial-to-Mesenchymal Transition by Stimulating Nuclear Factor-Kappa B and Is Associated with Poor Prognosis in Human Oral Squamous Cell Carcinoma. PMCID:    Pubmed No.:24999732 Wnt3a increases the metastatic potential of non-small cell lung cancer cells in vitro in part via its upregulation of Notch3 PMCID:    Pubmed No.:25572698 Proton pump inhibitor pantoprazole abrogates adriamycin-resistant gastric cancer cell invasiveness via suppression of Akt/GSK-β/β-catenin signaling and epithelial PMCID:    Pubmed No.:25449432 Mycoplasma hyorhinis induces epithelial-mesenchymal transition in gastric cancer cell MGC803 via TLR4-NF-κB signaling PMCID:    Pubmed No.:25149064 HES1 promotes metastasis and predicts poor survival in patients with colorectal cancer PMCID:    Pubmed No.:25636905 Beta-casomorphin-7 prevents epithelial-mesenchymal transdifferentiation of NRK-52E cells at high glucose level: Involvement of AngII-TGF-β1 pathway PMCID:    Pubmed No.:25882007 Specific chemotherapeutic agents induce metastatic behaviour through stromal‐and tumour‐derived cytokine and angiogenic factor signalling PMCID:    Pubmed No.:25988668 TGFβ1 secreted by cancer-associated fibroblasts induces epithelial-mesenchymal transition of bladder cancer cells through lncRNA-ZEB2NAT PMCID:    Pubmed No.:26152796 The Effect of cAMP-PKA Activation on TGF-β1-Induced Profibrotic Signaling PMCID:    Pubmed No.:26202352 The effect of TGF-beta-induced epithelial-mesenchymal transition on the expression of intracellular calcium-handling proteins in T47D and MCF-7 human breast cancer cells. PMCID:    Pubmed No.:26247838 Suppression of motor protein KIF3C expression inhibits tumor growth and metastasis in breast cancer by inhibiting TGF-β signaling PMCID:    Pubmed No.:26272184 LncRNA‐Hh Strengthen Cancer Stem Cells Generation in Twist‐Positive Breast Cancer via Activation of Hedgehog Signaling Pathway PMCID:    Pubmed No.:26418365 Paeonol inhibits B16F10 melanoma metastasis In vitro and In Vivo via disrupting proinflammatory cytokines‐mediated NF‐κB and STAT3 pathways PMCID:    Pubmed No.:26452780 Hepatitis C virus core protein interacts with Snail and histone deacetylases to promote the metastasis of hepatocellular carcinoma PMCID:    Pubmed No.:26549030 Decreasing lncRNA HOTAIR expression inhibits human colorectal cancer stem cells PMCID:    Pubmed No.:27069543 Cancer stem cell vaccine expressing ESAT-6-gpi and IL-21 inhibits melanoma growth and metastases PMCID:    Pubmed No.:26692931 The effect and mechanism of bufalin on regulating hepatocellular carcinoma cell invasion and metastasis via Wnt/β-catenin signaling pathway PMCID:    Pubmed No.:26648168 Oroxylin A inhibits invasion and migration through suppressing ERK/GSK‐3β signaling in snail‐expressing non‐small‐cell lung cancer cells PMCID:    Pubmed No.:26741501 Gastrointestinal Factor GDDR Attenuates Epithelial–Mesenchymal Transition in Gastric Cancer via Inhibiting AKT Signal PMCID:    Pubmed No.:27017226 Reinforcing B16F10/GPI-IL-21 vaccine efficacy against melanoma by injecting mice with shZEB1 plasmid or miR200c agomir PMCID:    Pubmed No.:25895132 Proton pump inhibitor pantoprazole abrogates adriamycin-resistant gastric cancer cell invasiveness via suppression of Akt/GSK-β/β-catenin signaling and epithelial PMCID:    Pubmed No.:25449432 Mycoplasma hyorhinis induces epithelial-mesenchymal transition in gastric cancer cell MGC803 via TLR4-NF-κB signaling PMCID:    Pubmed No.:25149064 Interaction between cyclooxygenase-2, Snail, and E-cadherin in gastric cancer cells PMCID:    Pubmed No.:24115825 LncRNA expression signatures of twist-induced epithelial-to-mesenchymal transition in MCF10A cells PMCID:    Pubmed No.:24113349 H. pylori infection-induced MSC differentiation into CAFs promotes epithelial-mesenchymal transition in gastric epithelial cells PMCID:    Pubmed No.:24145921 LncRNA‐Hh Strengthen Cancer Stem Cells Generation in Twist‐Positive Breast Cancer via Activation of Hedgehog Signaling Pathway PMCID:    Pubmed No.:26418365 Paeonol inhibits B16F10 melanoma metastasis In vitro and In Vivo via disrupting proinflammatory cytokines‐mediated NF‐κB and STAT3 pathways PMCID:    Pubmed No.:26452780 Hepatitis C virus core protein interacts with Snail and histone deacetylases to promote the metastasis of hepatocellular carcinoma PMCID:    Pubmed No.:26549030 Decreasing lncRNA HOTAIR expression inhibits human colorectal cancer stem cells PMCID:    Pubmed No.:27069543 Cancer stem cell vaccine expressing ESAT-6-gpi and IL-21 inhibits melanoma growth and metastases PMCID:    Pubmed No.:26692931 The effect and mechanism of bufalin on regulating hepatocellular carcinoma cell invasion and metastasis via Wnt/β-catenin signaling pathway PMCID:    Pubmed No.:26648168 Oroxylin A inhibits invasion and migration through suppressing ERK/GSK‐3β signaling in snail‐expressing non‐small‐cell lung cancer cells PMCID:    Pubmed No.:26741501 Gastrointestinal Factor GDDR Attenuates Epithelial–Mesenchymal Transition in Gastric Cancer via Inhibiting AKT Signal PMCID:    Pubmed No.:27017226 Reinforcing B16F10/GPI-IL-21 vaccine efficacy against melanoma by injecting mice with shZEB1 plasmid or miR200c agomir PMCID:    Pubmed No.:25895132 Down-regulating ribonuclease inhibitor enhances metastasis of bladder cancer cells through regulating epithelial-mesenchymal transition and ILK signaling pathway PMCID:    Pubmed No.:24768914 PMCID:    Pubmed No.:24812621 Benidipine Protects Kidney through Inhibiting ROCK1 Activity and Reducing the Epithelium-Mesenchymal Transdifferentiation in Type 1 Diabetic Rats PMCID:    Pubmed No.:24364038 Epigenetic silencing of SFRP1 and SFRP5 by hepatitis B virus X protein enhances hepatoma cell tumorigenicity through Wnt signaling pathway PMCID:    Pubmed No.:24374650 Synergism from the combination of ulinastatin and curcumin offers greater inhibition against colorectal cancer liver metastases via modulating matrix metalloproteinase-9 and E-cadherin expression. PMCID:    Pubmed No.:24570592 TGFbetaR2 is a major target of miR-93 in nasopharyngeal carcinoma aggressiveness PMCID:    Pubmed No.:24606633 Regulation gene expression of miR200c and ZEB1 positively enhances effect of tumor vaccine B16F10/GPI-IL-21 on inhibition of melanoma growth and metastasis PMCID:    Pubmed No.:24625224 Oroxylin A inhibits hypoxia-induced invasion and migration of MCF-7 cells by suppressing the Notch pathway PMCID:    Pubmed No.:24637576 Evidence of TGF-β 1 mediated epithelial-mesenchymal transition in immortalized benign prostatic hyperplasia cells PMCID:    Pubmed No.:24650126 Id-1, a Protein Repressed by miR-29b, Facilitates the TGFβ1-Induced Epithelial-Mesenchymal Transition in Human Ovarian Cancer Cells PMCID:    Pubmed No.:24662327 Acquisition of epithelial-mesenchymal transition phenotype in the tamoxifen-resistant breast cancer cell: a new role for G protein-coupled estrogen receptor in mediating tamoxifen resistance through cancer-associated fibroblast-derived fibronectin and β1- PMCID:    Pubmed No.:25990368 HBx protein‐mediated ATOH1 downregulation suppresses ARID2 expression and promotes hepatocellular carcinoma PMCID:    Pubmed No.:28498550 AEG‐1 induces gastric cancer metastasis by upregulation of eIF4E expression PMCID:    Pubmed No.:28661037 Claudin-4 is required for modulation of paracellular permeability by muscarinic acetylcholine receptor in epithelial cells PMCID:    Pubmed No.:25948584 TGFβ1 secreted by cancer-associated fibroblasts induces epithelial-mesenchymal transition of bladder cancer cells through lncRNA-ZEB2NAT PMCID:    Pubmed No.:26152796 miR520c blocks EMT progression of human breast cancer cells by repressing STAT3 PMCID:    Pubmed No.:28112380 Wnt3a increases the metastatic potential of non-small cell lung cancer cells in vitro in part via its upregulation of Notch3 PMCID:    Pubmed No.:25572698 Transcriptional silencing of ETS-1 abrogates epithelial-mesenchymal transition resulting in reduced motility of pancreatic cancer cells PMCID:    Pubmed No.:25421630 Synuclein-γ promotes migration of MCF7 breast cancer cells by activating extracellular-signal regulated kinase pathway and breaking cell-cell junctions PMCID:    Pubmed No.:25997706 Honokiol inhibits bladder cancer cell invasion through repressing SRC‑3 expression and epithelial‑mesenchymal transition PMCID:    Pubmed No.:28943942 Ginsenoside Rb1 inhibits hypoxia-induced epithelial‑mesenchymal transition in ovarian cancer cells by regulating microRNA-25 PMCID:    Pubmed No.:28928801 p70S6K promotes IL-6-induced epithelial-mesenchymal transition and metastasis of head and neck squamous cell carcinoma PMCID:    Pubmed No.:27174914 LncRNA SNHG15 acts as a ceRNA to regulate YAP1-Hippo signaling pathway by sponging miR-200a-3p in papillary thyroid carcinoma PMCID:    Pubmed No.:30237435 Glibenclamide Targets Sulfonylurea Receptor 1 to Inhibit p70S6K Activity and Upregulate KLF4 Expression to Suppress Non-Small Cell Lung Carcinoma PMCID:    Pubmed No.:31341030 Migration inhibition of water stress proteins from Nostoc commune Vauch. via activation of autophagy in DLD-1 cells PMCID:    Pubmed No.:30071226 NEAT1/miR-101-dependent Up-regulation of DNA-PKcs Enhances Malignant Behaviors of Pancreatic Ductal Adenocarcinoma Cells PMCID:    Pubmed No.:34405022 WDR1 Promotes Cell Growth and Migration and Contributes to Malignant Phenotypes of Non-small Cell Lung Cancer through ADF/cofilin-mediated Actin Dynamics PMCID:    Pubmed No.:29989053 miR-7-5p acts as a tumor suppressor in bladder cancer by regulating the hedgehog pathway factor Gli3 PMCID:    Pubmed No.:30100065 Plasmodium infection prevents recurrence and metastasis of hepatocellular carcinoma possibly via inhibition of the epithelial‑mesenchymal transition PMCID:    Pubmed No.:33846776 WDR1-regulated actin dynamics is required for outflow tract and right ventricle development PMCID:    Pubmed No.:29654745 Knockout of Wdr1 results in cardiac hypertrophy and impaired cardiac function in adult mouse heart PMCID:    Pubmed No.:30794912 Macrophages activate mesenchymal stem cells to acquire cancer‑associated fibroblast‑like features resulting in gastric epithelial cell lesions and malignant transformation in vitro PMCID:    Pubmed No.:30655826 miR‑152 regulates TGF‑β1‑induced epithelial‑mesenchymal transition by targeting HPIP in tubular epithelial cells PMCID:    Pubmed No.:29620271 Role of miR-21 in the growth and metastasis of human salivary adenoid cystic carcinoma PMCID:    Pubmed No.:29328455 Long non-coding RNA ESCCAL-1/miR-590/LRP6 signaling pathway participates in the progression of esophageal squamous cell carcinoma PMCID:    Pubmed No.:35655441 Simiao pill inhibits epithelial mesenchymal transition in a mouse model of chronic hyperuricemic nephropathy by inhibiting NLRP3 inflammasome activation PMCID:    Pubmed No.:36271349 Bacterial infection promotes tumorigenesis of colorectal cancer via regulating CDC42 acetylation PMCID:    Pubmed No.:36812247 Delivery of miR-3529-3p using MnO2-SiO2-APTES nanoparticles combined with phototherapy suppresses lung adenocarcinoma progression by targeting HIGD1A PMCID:    Pubmed No.:36808485 Exosomal hsa_circ_000200 as a potential biomarker and metastasis enhancer of gastric cancer via miR-4659a/b-3p/HBEGF axis PMCID:    Pubmed No.:37525152 Plasmodium immunotherapy combined with gemcitabine has a synergistic inhibitory effect on tumor growth and metastasis in murine Lewis lung cancer models PMCID:    Pubmed No.:37916167
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

Price/Size :

USD 368/1mg/vial



Tips: 

For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).

Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 


Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.
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