Cytochrome c (H19) polyclonal antibody 
Datasheet
COA
MSDS
SHIP
Product Name :
Cytochrome c (H19) polyclonal antibody Background :
Cytochrome C is an electron transporting protein that resides within the intermembrane space of the mitochondria, where it plays a critical role in the process of oxidative phosphorylation and production of cellular ATP. An increasing amount of interest has been directed toward the role which cytocrome C has been demonstrated to play in apoptotic processes. Following exposure to apoptotic stimuli, cytochrome C is rapidly released from the mitochondria into the cytosol, an event which may be required for the completion of apoptosis in some systems. Cytosolic cytochrome C functions in the activation of caspase 3, an ICE family molecule that is a key effector of apoptosis. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
Cytochrome c (H19) polyclonal antibody detects endogenous levels of Cytochrome c protein. Immunogen :
Synthetic peptide, corresponding to the N-terminal of Human Cytochrome c. Conjugate :
Unconjugated Modification :
Unmodification
Cytochrome c (H19) polyclonal antibody Background :
Cytochrome C is an electron transporting protein that resides within the intermembrane space of the mitochondria, where it plays a critical role in the process of oxidative phosphorylation and production of cellular ATP. An increasing amount of interest has been directed toward the role which cytocrome C has been demonstrated to play in apoptotic processes. Following exposure to apoptotic stimuli, cytochrome C is rapidly released from the mitochondria into the cytosol, an event which may be required for the completion of apoptosis in some systems. Cytosolic cytochrome C functions in the activation of caspase 3, an ICE family molecule that is a key effector of apoptosis. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
Cytochrome c (H19) polyclonal antibody detects endogenous levels of Cytochrome c protein. Immunogen :
Synthetic peptide, corresponding to the N-terminal of Human Cytochrome c. Conjugate :
Unconjugated Modification :
Unmodification
-
Western blot (WB) analysis of Cytochrome c (H19) polyclonal antibody at 1:500 dilution Lane1:HEK293T whole cell lysate(40ug) Lane2:Hela whole cell lysate(40ug) Lane3:RAW264.7 whole cell lysate(40ug) Lane4:PC12 whole cell lysate(40ug) -
Immunohistochemistry (IHC) analyzes of Cytochrome c (H19) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.
Coupling of mitochondrial function and skeletal muscle fiber type by a miR‐499/Fnip1/AMPK circuit
PMCID: Pubmed No.:27506764
ZL11n is a novel nitric oxide-releasing derivative of farnesylthiosalicylic acid that induces apoptosis in human hepatoma HepG2 cells via MAPK/mitochondrial pathways
PMCID: Pubmed No.:21621522
Xanthatin Induces Cell Cycle Arrest at G2/M Checkpoint and Apoptosis via Disrupting NF-κB Pathway in A549 Non-Small-Cell Lung Cancer Cells
PMCID: Pubmed No.:22450683
MicroRNA-145 Protects Cardiomyocytes against Hydrogen Peroxide (H2O2)-Induced Apoptosis through Targeting the Mitochondria Apoptotic Pathway
PMCID: Pubmed No.:23028672
Decoy receptor 3 suppresses RANKL-induced osteoclastogenesis via down-regulating NFATc1 and enhancing cell apoptosis
PMCID: Pubmed No.:23221330
Role of mitochondrial fission in neuronal injury in pilocarpine-induced epileptic rats
PMCID: Pubmed No.:23597828
Active extracts of black tea (Camellia Sinensis) induce apoptosis of PC-3 prostate cancer cells via mitochondrial dysfunction.
PMCID: Pubmed No.:23715786
Norcantharidin Induced DU145 Cell Apoptosis through ROS-Mediated Mitochondrial Dysfunction and Energy Depletion
PMCID: Pubmed No.:24367681
Norisoboldine induces apoptosis of fibroblast-like synoviocytes from adjuvant-induced arthritis rats
PMCID: Pubmed No.:24613208
Gastrodin Inhibits Glutamate-Induced Apoptosis of PC12 Cells via Inhibition of CaMKII/ASK-1/p38 MAPK/p53 Signaling Cascade
PMCID: Pubmed No.:24619207
NG as a novel nitric oxide donor induces apoptosis by increasing reactive oxygen species and inhibiting mitochondrial function in MGC803 cells
PMCID: Pubmed No.:25135879
8-Methoxypsoralen Induces Intrinsic Apoptosis in HepG2 Cells: Involvement of Reactive Oxygen Species Generation and ERK1/2 Pathway Inhibition
PMCID: Pubmed No.:26316083
Chamaejasmin B exerts anti-MDR effect in vitro and in vivo via initiating mitochondria-dependant intrinsic apoptosis pathway
PMCID: Pubmed No.:26445529
Alpinetin inhibits lung cancer progression and elevates sensitization drug-resistant lung cancer cells to cis-diammined dichloridoplatium
PMCID: Pubmed No.:26604699
Neuroprotective Effect of Human Adipose Stem Cell-Derived Extract in Amyotrophic Lateral Sclerosis
PMCID: Pubmed No.:26646002
The Pivotal Role of Ca2+ Homeostasis in PBDE-47-Induced Neuronal Apoptosis
PMCID: Pubmed No.:26676572
Excessive apoptosis and defective autophagy contribute to developmental testicular toxicity induced by fluoride
PMCID: Pubmed No.:26840522
NG as a novel nitric oxide donor induces apoptosis by increasing reactive oxygen species and inhibiting mitochondrial function in MGC803 cells
PMCID: Pubmed No.:25135879
Chamaejasmin B exerts anti-MDR effect in vitro and in vivo via initiating mitochondria-dependant intrinsic apoptosis pathway
PMCID: Pubmed No.:26445529
Alpinetin inhibits lung cancer progression and elevates sensitization drug-resistant lung cancer cells to cis-diammined dichloridoplatium
PMCID: Pubmed No.:26604699
Neuroprotective Effect of Human Adipose Stem Cell-Derived Extract in Amyotrophic Lateral Sclerosis
PMCID: Pubmed No.:26646002
The Pivotal Role of Ca2+ Homeostasis in PBDE-47-Induced Neuronal Apoptosis
PMCID: Pubmed No.:26676572
Excessive apoptosis and defective autophagy contribute to developmental testicular toxicity induced by fluoride
PMCID: Pubmed No.:26840522
Norcantharidin Induced DU145 Cell Apoptosis through ROS-Mediated Mitochondrial Dysfunction and Energy Depletion
PMCID: Pubmed No.:24367681
Norisoboldine induces apoptosis of fibroblast-like synoviocytes from adjuvant-induced arthritis rats
PMCID: Pubmed No.:24613208
Gastrodin Inhibits Glutamate-Induced Apoptosis of PC12 Cells via Inhibition of CaMKII/ASK-1/p38 MAPK/p53 Signaling Cascade
PMCID: Pubmed No.:24619207
Exercise Inducible Lactate Dehydrogenase B Regulates Mitochondrial Function in Skeletal Muscle
PMCID: Pubmed No.:27738103
AMPK-dependent and -independent coordination of mitochondrial function and muscle fiber type by FNIP1
PMCID: Pubmed No.:33780446
Apoptosis Induction by Iron Radiation via Inhibition of Autophagy in Trp53+/-Mouse Testes: Is Chronic Restraint-Induced Stress a Modifying Factor?
PMCID: Pubmed No.:29989073
Ameliorative effects and mechanisms of salvianic acid A on retinal iron overload in vivo and in vitro
PMCID: Pubmed No.:34058232
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.
Price/Size :
USD 368/1mg/vial
Tips:
For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).Describe :
Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.Formula:
Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.
Storage:
The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C.
Note :
This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.