Cyclin A (W250) polyclonal antibody 
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Product Name :
Cyclin A (W250) polyclonal antibody Background :
The critical role that the family of regulatory proteins known as cyclins play in eukaryotic cell cycle regulation is well established. The best-characterized cyclin complex is the mitotic cyclin B/Cdc2 p34 kinase, the active component of maturing promoting factor. Cyclin A accumulates prior to cyclin B in the cell cycle, appears to be involved in control of S phase and has been shown to associate with cyclin-dependent kinase-2 (Cdk2). In addition, cyclin A has been implicated in cell transformation and is found in complexes with E1A, transcription factors DRTF1 and E2F and retinoblastoma protein, p110. A second form of cyclin A, named cyclin A1 because of its high sequence homology to Xenopus cyclin A1, is most highly expressed in germ cells. It has been proposed that cyclin A1 can associate with Cdk2, p39 and Cdc2 p34. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
Cyclin A (W250) polyclonal antibody detects endogenous levels of Cyclin A protein. Immunogen :
Synthetic peptide, corresponding to amino acids 220-270 of Human Cyclin A. Conjugate :
Unconjugated Modification :
Unmodification
Cyclin A (W250) polyclonal antibody Background :
The critical role that the family of regulatory proteins known as cyclins play in eukaryotic cell cycle regulation is well established. The best-characterized cyclin complex is the mitotic cyclin B/Cdc2 p34 kinase, the active component of maturing promoting factor. Cyclin A accumulates prior to cyclin B in the cell cycle, appears to be involved in control of S phase and has been shown to associate with cyclin-dependent kinase-2 (Cdk2). In addition, cyclin A has been implicated in cell transformation and is found in complexes with E1A, transcription factors DRTF1 and E2F and retinoblastoma protein, p110. A second form of cyclin A, named cyclin A1 because of its high sequence homology to Xenopus cyclin A1, is most highly expressed in germ cells. It has been proposed that cyclin A1 can associate with Cdk2, p39 and Cdc2 p34. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
Cyclin A (W250) polyclonal antibody detects endogenous levels of Cyclin A protein. Immunogen :
Synthetic peptide, corresponding to amino acids 220-270 of Human Cyclin A. Conjugate :
Unconjugated Modification :
Unmodification
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Western blot (WB) analysis of Cyclin A (W250) pAb at 1:500 dilution Lane1:K562 whole cell lysate(40ug) Lane2:SGC7901 whole cell lysate(40ug) Lane3:The Testis tissue lysate of Mouse(40ug) Lane4:The Testis tissue lysate of Rat(40ug) -
Immunohistochemistry (IHC) analyzes of Cyclin A (W250) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.
Lewis y Regulate Cell Cycle Related Factors in Ovarian Carcinoma Cell RMG-I in Vitro via ERK and Akt Signaling Pathways
PMCID: Pubmed No.:22312289
Lewis y Regulate Cell Cycle Related Factors in Ovarian Carcinoma Cell RMG-I in Vitro via ERK and Akt Signaling Pathways
PMCID: Pubmed No.:22312289
Low Molecular Weight Apple Polysaccharides Induced Cell Cycle Arrest in Colorectal Tumor
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Effects of PEG-liposomal oxaliplatin on apoptosis, and expression of Cyclin A and Cyclin D1 in colorectal cancer cells.
PMCID: Pubmed No.:22710431
Euphol arrests breast cancer cells at the G1 phase through the modulation of cyclin D1, p21 and p27 expression
PMCID: Pubmed No.:23969579
GPER mediates enhanced cell viability and motility via non-genomic signaling induced by 17β-estradiol in triple-negative breast cancer cells
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Selective ligands of estrogen receptor β discovered using pharmacophore mapping and structure-based virtual screening
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Wogonoside induces growth inhibition and cell cycle arrest via promoting the expression and binding activity of GATA-1 in chronic myelogenous leukemia cells
PMCID: Pubmed No.:26104856
Suppression of RAD21 induces senescence of MDA-MB-231 human breast cancer cells through RB1 pathway activation via c-Myc downregulation.
PMCID: Pubmed No.:26529363
Raltitrexed Inhibits HepG2 Cell Proliferation via G0/G1 Cell Cycle Arrest
PMCID: Pubmed No.:22415097
Selective ligands of estrogen receptor β discovered using pharmacophore mapping and structure-based virtual screening
PMCID: Pubmed No.:25176400
Suppression of RAD21 induces senescence of MDA-MB-231 human breast cancer cells through RB1 pathway activation via c-Myc downregulation.
PMCID: Pubmed No.:26529363
Raltitrexed Inhibits HepG2 Cell Proliferation via G0/G1 Cell Cycle Arrest
PMCID: Pubmed No.:22415097
Raltitrexed Inhibits HepG2 Cell Proliferation via G0/G1 Cell Cycle Arrest
PMCID: Pubmed No.:27098147
Wogonoside induces growth inhibition and cell cycle arrest via promoting the expression and binding activity of GATA-1 in chronic myelogenous leukemia cells
PMCID: Pubmed No.:26104856
Suppression of RAD21 Induces Senescence of MDA‐MB‐231 Human Breast Cancer Cells Through RB1 Pathway Activation Via c‐Myc Downregulation
PMCID: Pubmed No.:26529363
ONTD induces growth arrest and apoptosis of human hepatoma Bel-7402 cells though a peroxisome proliferator-activated receptor γ-dependent pathway
PMCID: Pubmed No.:28834734
Vacuolar protein sorting 4B regulates the proliferation and odontoblastic differentiation of human dental pulp stem cells through the Wnt-β-catenin signalling pathway
PMCID: Pubmed No.:31218890
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.
Price/Size :
USD 368/1mg/vial
Tips:
For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).Describe :
Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.Formula:
Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.
Storage:
The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C.
Note :
This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.