Contact us : info@bioworlde.com
Home > Product > Primary Antibodies
Cox-2 (T594) polyclonal antibody BS1076
  • Western blot (WB) analysis of Cox-2 (T594) polyclonal antibody at 1:500 dilution Lane1:CT26 whole cell lysate(20ug) Lane2:PC12 whole cell lysate(40ug) Lane3:Hela whole cell lysate(40ug)
  • Immunohistochemistry (IHC) analyzes of Cox-2 (T594) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.
Product NameCox-2 (T594) polyclonal antibody
Catalog No.BS1076
Swiss-ProtP35354
Host Rabbit
ReactivityHuman,Mouse,Rat
ApplicationsWB IHC IF
Application_allWB: 1:500~1:1000 IHC: 1:50~1:200 IF: 1:50~1:200
BiowMW~ 74 kDa
Alternative NameProstaglandin G/H synthase 2; Cyclooxygenase-2; COX-2; PHS II; Prostaglandin H2 synthase 2; PGH synthase 2; PGHS-2; PGHS2; Prostaglandin-endoperoxide synthase 2; PTGS2; COX2
Purification&PurityThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
ConjugateUnconjugated
ModificationUnmodification
Browse similar products>>
Size Price
50ul $158
100ul $275
Add to cart My orders
Product Name :
Cox-2 (T594) polyclonal antibody
Background :
Prostaglandins are a diverse group of autocrine and paracrine hormones that mediate many cellular and physiologic processes. Prostaglandin H2 (PGH2) is an intermediate in formation of the prostaglandins. Two prostaglandin synthases that catalyze the formation of PGH2 from arachidonic acid (AA) are cyclooxygenase-1 and cyclooxygenase-2. Cyclooxygenase-2, or Cox-2, is efficiently induced in migratory cells responding to pro-inflammatory stimuli and is considered to be an important mediator of inflammation. An alternative form of the protein, designated Cox-1, is constitutively expressed in most tissues and is thought to serve in general “housekeeping” functions. Both enzymes are targets for the nonsteroidal therapeutic anti-inflammatorydrugs, NSAIDs.
Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity :
Cox2 (T594) polyclonal antibody detects endogenous levels of Cox2 protein.
Immunogen :
Synthetic peptide, corresponding to amino acids C-terminus of Human Cox-2.
Conjugate :
Unconjugated
Modification :
Unmodification
  • Western blot (WB) analysis of Cox-2 (T594) polyclonal antibody at 1:500 dilution Lane1:CT26 whole cell lysate(20ug) Lane2:PC12 whole cell lysate(40ug) Lane3:Hela whole cell lysate(40ug)
  • Immunohistochemistry (IHC) analyzes of Cox-2 (T594) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.
Synergistic effect of 5-fluorouracil and the flavanoid oroxylin A on HepG2 human hepatocellular carcinoma and on H22 transplanted mice PMCID:    Pubmed No.:19585117 Enhanced anticancer effect of gemcitabine by genistein in osteosarcoma: the role of Akt and nuclear factor-kappaB PMCID:    Pubmed No.:20038829 Double antioxidant activities of rosiglitazone against high glucose-induced oxidative stress in hepatocyte PMCID:    Pubmed No.:21333731 Oxidative Stress Mediates Chemical Hypoxia-Induced Injury and Inflammation by Activating NF-κb-COX-2 Pathway in HaCaT Cells PMCID:    Pubmed No.:21533553 Hydrogen Sulfide Protects against Chemical Hypoxia-Induced Cytotoxicity and Inflammation in HaCaT Cells through Inhibition of ROS/NF-kB/COX-2 Pathway PMCID:    Pubmed No.:21779360 Luteolin protects against reactive oxygen species-mediated cell death induced by zinc toxicity via the PI3K–Akt–NF-κB–ERK-dependent pathway PMCID:    Pubmed No.:21800350 Omega-3 docosahexaenoic acid and procyanidins inhibit cyclooxygenase activity and attenuate NF-κB activation through a p105/p50 regulatory mechanism in macrophage inflammation PMCID:    Pubmed No.:21954853 Genistein potentiates the anti-cancer effects of gemcitabine in human osteosarcoma via the downregulation of Akt and nuclear factor-κB pathway. PMCID:    Pubmed No.:22263786 Protective effect of L-theanine on carbon tetrachloride-induced acute liver injury in mice PMCID:    Pubmed No.:22583898 Additive Effects of Ulinastatin and Docetaxel on Growth of Breast Cancer Xenograft in Nude Mice and Expression of PGE2, IL-10, and IL-2 in Primary Breast Cancer Cells PMCID:    Pubmed No.:22594905 Oroxylin A improves the sensitivity of HT-29 human colon cancer cells to 5-FU through modulation of the COX-2 signaling pathway PMCID:    Pubmed No.:22607196 Oxidative Burst of Circulating Neutrophils Following Traumatic Brain Injury in Human PMCID:    Pubmed No.:23894384 Interaction between cyclooxygenase-2, Snail, and E-cadherin in gastric cancer cells PMCID:    Pubmed No.:24115825 Cobalt Protoporphyrin Induces HO-1 Expression Mediated Partially by FOXO1 and Reduces Mitochondria-Derived Reactive Oxygen Species Production PMCID:    Pubmed No.:24255720 The Adverse Effect of Selective Cyclooxygenase-2 Inhibitor on Random Skin Flap Survival in Rats PMCID:    Pubmed No.:24324831 FOXP3 inhibits NF-κB activity and hence COX2 expression in gastric cancer cells PMCID:    Pubmed No.:24308961 Urocortin increased endothelial ICAM1 by cPLA2-dependent NF-κB and PKA pathways in HUVECs PMCID:    Pubmed No.:24363440 Effects of Cichoric Acid Extract from Echinacea purpurea on Collagen-Induced Arthritis in Rats PMCID:    Pubmed No.:24871659 Phenolcarboxylic acids from medicinal herbs exert anticancer effects through disruption of COX-2 activity PMCID:    Pubmed No.:24916702 Effect of celecoxib combined with chemotherapy drug on malignant biological behaviors of gastric cancer PMCID:    Pubmed No.:25550798 Procyanidin B2 inhibits inflammasome-mediated IL-1β production in lipopolysaccharide-stimulated macrophages. PMCID:    Pubmed No.:25379992 Alpha-Tomatine Exhibits Anti-inflammatory Activity in Lipopolysaccharide-Activated Macrophages PMCID:    Pubmed No.:25772114 Sulfiredoxin-1 protects primary cultured astrocytes from ischemia-induced damage PMCID:    Pubmed No.:25620665 Cancer-promoting effect of capsaicin on DMBA/TPA-induced skin tumorigenesis by modulating inflammation, Erk and P38 in mice PMCID:    Pubmed No.:25846502 Chemopreventive efficacy of menthol on carcinogen-induced cutaneous carcinoma through inhibition of inflammation and oxidative stress in mice PMCID:    Pubmed No.:25956868 Esculentoside A suppresses Aβ1-42-induced neuroinflammation by down-regulating MAPKs pathways in vivo PMCID:    Pubmed No.:26104317 Resveratrol prevents alveolar bone loss in an experimental rat model of periodontitis PMCID:    Pubmed No.:26497626 Nano-microcapsule basic fibroblast growth factor combined with hypoxia-inducible factor-1 improves random skin flap survival in rats PMCID:    Pubmed No.:26707180 The effect and mechanism of bufalin on regulating hepatocellular carcinoma cell invasion and metastasis via Wnt/β-catenin signaling pathway PMCID:    Pubmed No.:26648168 TATA boxes in gene transcription and poly (A) tails in mRNA stability: New perspective on the effects of berberine PMCID:    Pubmed No.:26671652 Targeted deletion of miR‐139‐5p activates MAPK, NF‐κB and STAT3 signaling and promotes intestinal inflammation and colorectal cancer PMCID:    Pubmed No.:26859226 Procyanidin B2 inhibits inflammasome-mediated IL-1β production in lipopolysaccharide-stimulated macrophages. PMCID:    Pubmed No.:25379992 Interaction between cyclooxygenase-2, Snail, and E-cadherin in gastric cancer cells PMCID:    Pubmed No.:24115825 Cobalt Protoporphyrin Induces HO-1 Expression Mediated Partially by FOXO1 and Reduces Mitochondria-Derived Reactive Oxygen Species Production PMCID:    Pubmed No.:24255720 Resveratrol prevents alveolar bone loss in an experimental rat model of periodontitis PMCID:    Pubmed No.:26497626 Nano-microcapsule basic fibroblast growth factor combined with hypoxia-inducible factor-1 improves random skin flap survival in rats PMCID:    Pubmed No.:26707180 The effect and mechanism of bufalin on regulating hepatocellular carcinoma cell invasion and metastasis via Wnt/β-catenin signaling pathway PMCID:    Pubmed No.:26648168 TATA boxes in gene transcription and poly (A) tails in mRNA stability: New perspective on the effects of berberine PMCID:    Pubmed No.:26671652 Targeted deletion of miR‐139‐5p activates MAPK, NF‐κB and STAT3 signaling and promotes intestinal inflammation and colorectal cancer PMCID:    Pubmed No.:26859226 The Adverse Effect of Selective Cyclooxygenase-2 Inhibitor on Random Skin Flap Survival in Rats PMCID:    Pubmed No.:24324831 FOXP3 inhibits NF-κB activity and hence COX2 expression in gastric cancer cells PMCID:    Pubmed No.:24308961 Urocortin increased endothelial ICAM1 by cPLA2-dependent NF-κB and PKA pathways in HUVECs PMCID:    Pubmed No.:24363440 Anti-inflammatory, anti-osteoclastic, and antioxidant activities of genistein protect against alveolar bone loss and periodontal tissue degradation in a mouse model of periodontitis PMCID:    Pubmed No.:28509410 Targeted deletion of miR‐139‐5p activates MAPK, NF‐κB and STAT3 signaling and promotes intestinal inflammation and colorectal cancer PMCID:    Pubmed No.:26859226 COMP-Ang1 prevents periodontitic damages and enhances mandible bone growth in an experimental animal model PMCID:    Pubmed No.:27612438 Anti‑inflammatory effects of Fagopyrum cymosum administered as a potential drug for ulcerative colitis PMCID:    Pubmed No.:29201175 Tumor-associated macrophages induce vasculogenic mimicry of glioblastoma multiforme through cyclooxygenase-2 activation PMCID:    Pubmed No.:27824617 Prunus mume Seed Exhibits Inhibitory Effect on Skin Senescence via SIRT1 and MMP-1 Regulation PMCID:    Pubmed No.:34122721 TREM2 deficiency aggravates α-synuclein–induced neurodegeneration and neuroinflammation in Parkinson’s disease models PMCID:    Pubmed No.:31370707 A thiopyran derivative with low murine toxicity with therapeutic potential on lung cancer acting through a NF-κB mediated apoptosis-to-pyroptosis switch PMCID:    Pubmed No.:30519834 Oral supplementation with p-coumaric acid protects mice against diabetes-associated spontaneous destruction of periodontal tissue PMCID:    Pubmed No.:31328274 Cichorium pumilum Jacq Extract Inhibits LPS-Induced Inflammation via MAPK Signaling Pathway and Protects Rats From Hepatic Fibrosis Caused by Abnormalities in the Gut-Liver Axis PMCID:    Pubmed No.:33995112 Mas-related G protein-coupled receptor D participates in inflammatory pain by promoting NF-κB activation through interaction with TAK1 and IKK complex PMCID:    Pubmed No.:33080316 GDNF enhances the anti-inflammatory effect of human adipose-derived mesenchymal stem cell-based therapy in renal interstitial fibrosis PMCID:    Pubmed No.:31706095 Lipophilic Extract and Tanshinone IIA Derived from Salvia miltiorrhiza Attenuate Uric Acid Nephropathy through Suppressing Oxidative Stress-Activated MAPK Pathways PMCID:    Pubmed No.:32933312 Berberine Protects Secondary Injury in Mice with Traumatic Brain Injury Through Anti-oxidative and Anti-inflammatory Modulation PMCID:    Pubmed No.:30027364 Dapagliflozin protects against nonalcoholic steatohepatitis in db/db mice. PMCID:    Pubmed No.:36059948 Exposure of newborns to atmospherically relevant artificial particulate matter induces hematopoietic stem cell senescence PMCID:    Pubmed No.:37002998 (+)-Borneol exerts neuroprotective effects via suppressing the NF-κB pathway in the pilocarpine-induced epileptogenesis rat model PMCID:    Pubmed No.:37127175 Anti-oxidant and Anti-inflammatory Effects of Ethanol Extract from Polygala sibirica L. var megalopha Fr. on Lipopolysaccharide-Stimulated RAW264.7 Cells PMCID:    Pubmed No.:37434032
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

Price/Size :

USD 368/1mg/vial



Tips: 

For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).

Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 


Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.
COPYRIGHT © 2015-2018 Bioworld Technology, Inc. All rights reserved POLYCLONAL AND MONOCLONAL ANTIBODY CENTER