COL2A1 (P133) polyclonal antibody 
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Product Name :
COL2A1 (P133) polyclonal antibody Background :
The extensive family of COL gene products (collagens) is composed of several chain types, including fibril-forming interstitial collagens (types I, II, III and V) and basement membrane collagens (type IV), each type containing multiple isoforms. Collagens are fibrous, extracellular matrix proteins with high tensile strength and are the major components of connective tissue, such as tendons and cartilage. All collagens contain a triple helix domain and frequently show lateral self-association in order to form complex connective tissues. Several collagens also play a role in cell adhesion, important for maintaining normal tissue architecture and function. In cartilage, Collagen Type II constitutes the bulk of the fibril. Sensitization with Collagen Type II induces an erosive polyarthritis in rats, mice and higher primates which can resemble rheumatoid arthritis and relapsing polychrondritis. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
COL2A1 (P133) polyclonal antibody detects endogenous levels of COL2A1 protein Immunogen :
Synthetic peptide, corresponding to amino acids 101-150 of Human COL2A1. Conjugate :
Unconjugated Modification :
Unmodification
COL2A1 (P133) polyclonal antibody Background :
The extensive family of COL gene products (collagens) is composed of several chain types, including fibril-forming interstitial collagens (types I, II, III and V) and basement membrane collagens (type IV), each type containing multiple isoforms. Collagens are fibrous, extracellular matrix proteins with high tensile strength and are the major components of connective tissue, such as tendons and cartilage. All collagens contain a triple helix domain and frequently show lateral self-association in order to form complex connective tissues. Several collagens also play a role in cell adhesion, important for maintaining normal tissue architecture and function. In cartilage, Collagen Type II constitutes the bulk of the fibril. Sensitization with Collagen Type II induces an erosive polyarthritis in rats, mice and higher primates which can resemble rheumatoid arthritis and relapsing polychrondritis. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
COL2A1 (P133) polyclonal antibody detects endogenous levels of COL2A1 protein Immunogen :
Synthetic peptide, corresponding to amino acids 101-150 of Human COL2A1. Conjugate :
Unconjugated Modification :
Unmodification
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Immunohistochemistry (IHC) analyzes of COL2A1 (P133) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.
MicroRNA-145 attenuates TNF- α -driven cartilage matrix degradation in osteoarthritis via direct suppression of MKK4
PMCID: Pubmed No.:29072705
miR-146a facilitates osteoarthritis by regulating cartilage homeostasis via targeting Camk2d and Ppp3r2
PMCID: Pubmed No.:28383548
Nerve growth factor from Chinese cobra venom stimulates chondrogenic differentiation of mesenchymal stem cells
PMCID: Pubmed No.:28518137
The combined effects of continuous passive motion treatment and acellular PLGA implants on osteochondral regeneration in the rabbit
PMCID: Pubmed No.:22264523
All-trans-retinoid acid (ATRA) may have inhibited chondrogenesis of primary hind limb bud mesenchymal cells by downregulating Pitx1 expression.
PMCID: Pubmed No.:23810783
All-trans-retinoid acid (ATRA) may have inhibited chondrogenesis of primary hind limb bud mesenchymal cells by downregulating Pitx1 expression.
PMCID: Pubmed No.:23810783
Tougu Xiaotong formula induces chondrogenic differentiation in association with transforming growth factor-β1 and promotes proliferation in bone marrow stromal
PMCID: Pubmed No.:25543835
Study of differential properties of fibrochondrocytes and hyaline chondrocytes in growing rabbits
PMCID: Pubmed No.:25529749
Bushen Zhuangjin Decoction promotes chondrocyte proliferation by stimulating cell cycle progression
PMCID: Pubmed No.:25667638
The effects of static pressure on chondrogenic and osteogenic differentiation in condylar chondrocytes from temporomandibular joint
PMCID: Pubmed No.:25656086
Bushen Zhuangjin decoction inhibits TM-induced chondrocyte apoptosis mediated by endoplasmic reticulum stress
PMCID: Pubmed No.:26497741
Fuyuan Decoction Enhances SOX9 and COL2A1 Expression and Smad2/3 Phosphorylation in IL-1β-Activated Chondrocytes
PMCID: Pubmed No.:26770254
Altered function in cartilage derived mesenchymal stem cell leads to OA-related cartilage erosion
PMCID: Pubmed No.:27158337
Tougu Xiaotong formula induces chondrogenic differentiation in association with transforming growth factor-β1 and promotes proliferation in bone marrow stromal
PMCID: Pubmed No.:25543835
Study of differential properties of fibrochondrocytes and hyaline chondrocytes in growing rabbits
PMCID: Pubmed No.:25529749
Bushen Zhuangjin decoction inhibits TM-induced chondrocyte apoptosis mediated by endoplasmic reticulum stress
PMCID: Pubmed No.:26497741
Fuyuan Decoction Enhances SOX9 and COL2A1 Expression and Smad2/3 Phosphorylation in IL-1β-Activated Chondrocytes
PMCID: Pubmed No.:26770254
Psoralen promotes the expression of cyclin D1 in chondrocytes via the Wnt/β-catenin signaling pathway
PMCID: Pubmed No.:28949389
Tougu Xiaotong formula induces chondrogenic differentiation in association with transforming growth factor-β1 and promotes proliferation in bone marrow stromal cells
PMCID: Pubmed No.:25543835
Bushen Zhuangjin decoction inhibits TM-induced chondrocyte apoptosis mediated by endoplasmic reticulum stress
PMCID: Pubmed No.:26497741
All‑trans‑retinoic acid inhibits chondrogenesis of rat embryo hindlimb bud mesenchymal cells by downregulating p53 expression
PMCID: Pubmed No.:25738595
Electroacupuncture serum inhibits TNF‑α‑mediated chondrocyte inflammation via the Ras‑Raf‑MEK1/2‑ERK1/2 signaling pathway
PMCID: Pubmed No.:28849229
Fuyuan Decoction Enhances SOX9 and COL2A1 Expression and Smad2/3 Phosphorylation in IL-1β-Activated Chondrocytes
PMCID: Pubmed No.:26770254
The effects of static pressure on chondrogenic and osteogenic differentiation in condylar chondrocytes from temporomandibular joint
PMCID: Pubmed No.:25656086
Peroxisome Proliferator-Activated Receptor-γ Coactivator-1α Inhibits Vascular Calcification Through Sirtuin 3-Mediated Reduction of Mitochondrial Oxidative Stress
PMCID: Pubmed No.:30829051
Coniferaldehyde prevents articular cartilage destruction in a murine model via Nrf2/HO‑1 pathway
PMCID: Pubmed No.:33495836
Glucosamine promotes chondrocyte proliferation via the Wnt/β‑catenin signaling pathway
PMCID: Pubmed No.:29568900
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.
Price/Size :
USD 368/1mg/vial
Tips:
For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).Describe :
Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.Formula:
Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.
Storage:
The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C.
Note :
This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.