Claudin-4 (P192) polyclonal antibody 
Datasheet
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MSDS
SHIP
Product Name :
Claudin-4 (P192) polyclonal antibody Background :
The Claudin superfamily consists of many structurally related proteins in humans. These proteins are important structural and functional components of tight junctions in paracellular transport. Claudins are located in both epithelial and endothelial cells in all tight junction-bearing tissues. Three classes of proteins are known to localize to tight junctions, including the Claudins, Occludin and junction adhesion molecule (JAM). Claudins, which consist of four transmembrane domains and two extracellular loops, make up tight junction strands. Claudin expression is highly restricted to specfic regions of different tissues and variations of Claudin expression may have an important role in transcellular transport through tight junctions. In rat liver, claudin-3 is uniformly expressed, whereas in the pancreas, claudin-3 is expressed in junctions of the duct epithelia and junctions of acinar cells. Claudin-3 binds the peptide toxin Clostridium perfringens enterotoxin (CPE) at the cell surface via the second extracellular loop of claudin-3. The gene encoding human claudin-3 maps to chromosome 7q11.23. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
Claudin 4 (P192) polyclonal antibody detects endogenous levels of Claudin 4 protein. Immunogen :
Synthetic peptide, corresponding to amino acids 171-220 of Human Claudin-4. Conjugate :
Unconjugated Modification :
Unmodification
Claudin-4 (P192) polyclonal antibody Background :
The Claudin superfamily consists of many structurally related proteins in humans. These proteins are important structural and functional components of tight junctions in paracellular transport. Claudins are located in both epithelial and endothelial cells in all tight junction-bearing tissues. Three classes of proteins are known to localize to tight junctions, including the Claudins, Occludin and junction adhesion molecule (JAM). Claudins, which consist of four transmembrane domains and two extracellular loops, make up tight junction strands. Claudin expression is highly restricted to specfic regions of different tissues and variations of Claudin expression may have an important role in transcellular transport through tight junctions. In rat liver, claudin-3 is uniformly expressed, whereas in the pancreas, claudin-3 is expressed in junctions of the duct epithelia and junctions of acinar cells. Claudin-3 binds the peptide toxin Clostridium perfringens enterotoxin (CPE) at the cell surface via the second extracellular loop of claudin-3. The gene encoding human claudin-3 maps to chromosome 7q11.23. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
Claudin 4 (P192) polyclonal antibody detects endogenous levels of Claudin 4 protein. Immunogen :
Synthetic peptide, corresponding to amino acids 171-220 of Human Claudin-4. Conjugate :
Unconjugated Modification :
Unmodification
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Western blot (WB) analysis of Claudin-4 (P192) polyclonal antibody at 1:500 dilution Lane1:A549 whole cell lysate(40ug) Lane2:HCT116 whole cell lysate(40ug) Lane3:DLD whole cell lysate(40ug) -
Immunohistochemistry (IHC) analyzes of Claudin-4 (P192) pAb in paraffin-embedded human breast carcinoma tissue at 1:100. -
Immunohistochemistry (IHC) analyzes of Claudin-4 (P192) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.
Identification of Claudins by Western Blot and Immunofluorescence in Different Cell Lines and Tissues
PMCID: Pubmed No.:21717359
Protective role of uncoupling protein-2 against dextran sodium sulfate-induced colitis
PMCID: Pubmed No.:21871020
Claudin-3 is required for modulation of paracellular permeability by TNF-α through ERK1/2/slug signaling axis in submandibular gland
PMCID: Pubmed No.:26148935
Protective role of uncoupling protein-2 against dextran sodium sulfate-induced colitis
PMCID: Pubmed No.:21871020
Claudin-4 is required for modulation of paracellular permeability by muscarinic acetylcholine receptor in epithelial cells
PMCID: Pubmed No.:25948584
Parasympathectomy increases resting secretion of the submandibular gland in minipigs in the long term
PMCID: Pubmed No.:30387129
CTRP3 promotes TNF-α-induced apoptosis and barrier dysfunction in salivary epithelial cells
PMCID: Pubmed No.:33991612
Shp2 regulates PM2.5-induced airway epithelial barrier dysfunction by modulating ERK1/2 signaling pathway
PMCID: Pubmed No.:34252507
Disruption of tight junction structure contributes to secretory dysfunction in IgG4-related sialadenitis
PMCID: Pubmed No.:31865502
Expression patterns of tight junction proteins in porcine major salivary glands: a comparison study with human and murine glands
PMCID: Pubmed No.:29851087
Effect of ischemic preconditioning on radiation damage to the submandibular gland in rats
PMCID: Pubmed No.:33786924
Disruption of tight junctions contributes to hyposalivation of salivary glands in a mouse model of type 2 diabetes
PMCID: Pubmed No.:32374057
Alteration of tight junctions during botulinum toxin type A-inhibited salivary secretion
PMCID: Pubmed No.:35472254
High concentrate diet induced inflammatory response and tight junction disruption in the mammary gland of dairy cows
PMCID: Pubmed No.:36709006
A high-concentrate diet induces colonic inflammation and barrier damage in Hu sheep
PMCID: Pubmed No.:37641289
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.
Price/Size :
USD 368/1mg/vial
Tips:
For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).Describe :
Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.Formula:
Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.
Storage:
The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C.
Note :
This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.