Apaf-1-ALT (H324) polyclonal antibody 
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Product Name :
Apaf-1-ALT (H324) polyclonal antibody Background :
APAF-1-ALT harbors the caspase recruitment domain and an incomplete CED-4 like/ATPase domain, but lacks the WD-40 repeat units. The LNCaP cell expressed the full-length APAF-1 weakly and APAF-1-ALT rather abundantly, especially after mycoplasma infection. LNCaP underwent a retarded DNA damage-induced apoptosis, which was independent of caspase 9 activity. APAF-1-ALT functioned less effectively in inducing apoptosis than did APAF-1-XL, the full-length APAF-1, in transient transfection. Moreover, APAF-1-ALT interfered with APAF-1-XL's ability to induce apoptosis in transient double transfection experiment. These results indicate that APAF-1-ALT is a molecule that is deficient and impeded for mediating apoptosis and that it may contribute to the resistance to DNA damage-induced treatment observed in LNCaP. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
APAF-1-ALT(H324) polyclonal antibody detects endogenous levels of APAF-1 Isoform 6 (Apaf-1-ALT) protein. Immunogen :
Synthetic peptide, corresponding to amino acids 301-350 of Human Apaf-1-ALT. Conjugate :
Unconjugated Modification :
Unmodification
Apaf-1-ALT (H324) polyclonal antibody Background :
APAF-1-ALT harbors the caspase recruitment domain and an incomplete CED-4 like/ATPase domain, but lacks the WD-40 repeat units. The LNCaP cell expressed the full-length APAF-1 weakly and APAF-1-ALT rather abundantly, especially after mycoplasma infection. LNCaP underwent a retarded DNA damage-induced apoptosis, which was independent of caspase 9 activity. APAF-1-ALT functioned less effectively in inducing apoptosis than did APAF-1-XL, the full-length APAF-1, in transient transfection. Moreover, APAF-1-ALT interfered with APAF-1-XL's ability to induce apoptosis in transient double transfection experiment. These results indicate that APAF-1-ALT is a molecule that is deficient and impeded for mediating apoptosis and that it may contribute to the resistance to DNA damage-induced treatment observed in LNCaP. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
APAF-1-ALT(H324) polyclonal antibody detects endogenous levels of APAF-1 Isoform 6 (Apaf-1-ALT) protein. Immunogen :
Synthetic peptide, corresponding to amino acids 301-350 of Human Apaf-1-ALT. Conjugate :
Unconjugated Modification :
Unmodification
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Western blot (WB) analysis of APAF-1-ALT (H324) polyclonal antibody in extracts from COLO205 cells. -
Immunohistochemistry (IHC) analyzes of Apaf-1-ALT (H324) pAb in paraffin-embedded human lung carcinoma tissue at 1:50,showing cytoplasmic staining.Negative control (the right)Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.
MicroRNA-145 Protects Cardiomyocytes against Hydrogen Peroxide (H2O2)-Induced Apoptosis through Targeting the Mitochondria Apoptotic Pathway
PMCID: Pubmed No.:23028672
Alteration of apoptotic protease activating factor 1 expression and possible role in ONOO--induced apoptosis in human cerebral vascular smooth muscle cells.
PMCID: Pubmed No.:26770639
Alteration of apoptotic protease activating factor 1 expression and possible role in ONOO--induced apoptosis in human cerebral vascular smooth muscle cells.
PMCID: Pubmed No.:26770639
Alteration of apoptotic protease activating factor 1 expression and possible role in ONOO--induced apoptosis in human cerebral vascular smooth muscle cells
PMCID: Pubmed No.:26770639
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.
Price/Size :
USD 368/1mg/vial
Tips:
For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).Describe :
Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.Formula:
Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.
Storage:
The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C.
Note :
This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.