APOB polyclonal antibody 
Datasheet
COA
MSDS
SHIP
Product Name :
APOB polyclonal antibody Background :
Post-transcriptional editing of apolipoprotein B (apoB) mRNA is regulated by APOBEC1 (also designated human (or rat) small intestinal apolipoprotein B mRNA editing protein, HEPR, or REPR) in hepatic cells to achieve a steady state proportion of edited and unedited RNA molecules. Two forms of apoB are known to circulate in the plasma of mammals. apoB-100 is a protein primarily synthesized in the liver as a structural component of very-low-density lipoprotein particles. A truncated form of apoB-100, apoB-48, is synthesized in the small intestine and contains the amino-terminal 2,152 amino acids of the larger protein. This organ-specific partitioning of apoB production is the result of RNA editing of a common apoB gene. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
APOB polyclonal antibody detects endogenous levels of APOB protein. Immunogen :
Recombinant APOB protein, corresponding to amino acids 97-526 of Human APOB. Conjugate :
Unconjugated Modification :
Unmodification
APOB polyclonal antibody Background :
Post-transcriptional editing of apolipoprotein B (apoB) mRNA is regulated by APOBEC1 (also designated human (or rat) small intestinal apolipoprotein B mRNA editing protein, HEPR, or REPR) in hepatic cells to achieve a steady state proportion of edited and unedited RNA molecules. Two forms of apoB are known to circulate in the plasma of mammals. apoB-100 is a protein primarily synthesized in the liver as a structural component of very-low-density lipoprotein particles. A truncated form of apoB-100, apoB-48, is synthesized in the small intestine and contains the amino-terminal 2,152 amino acids of the larger protein. This organ-specific partitioning of apoB production is the result of RNA editing of a common apoB gene. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
APOB polyclonal antibody detects endogenous levels of APOB protein. Immunogen :
Recombinant APOB protein, corresponding to amino acids 97-526 of Human APOB. Conjugate :
Unconjugated Modification :
Unmodification
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Western blot (WB) analysis of APOB polyclonal antibody at 1:1000 dilution Lane1:Recombinant APOB protein, corresponding to amino acids 191-235 of Human APOB(10ng) Lane2:Recombinant APOB protein, corresponding to amino acids 191-235 of Human APOB.(20ng) Lane3:Recombinant APOB protein, corresponding to amino acids 191-235 of Human APOB.(50ng)
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.
Price/Size :
USD 368/1mg/vial
Tips:
For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).Describe :
Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.Formula:
Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.
Storage:
The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C.
Note :
This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.