Product Name :
Lamin B1 polyclonal antibody Background :
An important part of the nucleus is formed by nuclear lamina. Nuclear lamins form a network of filaments at the nucleoplasmic site of the nuclear membrane. Two main subtypes of nuclear lamins can be distinguished, i.e. A type lamins and B type lamins. The A type lamins comprise a set of three proteins arising from the same gene by alternative splicing, i.e. lamin A, lamin C and lamin Adel10, while the B-type lamins include two proteins arising from two distinct genes, i.e. lamin B1 and lamin B2. The nuclear lamins comprise a unique subclass of the intermediate filament protein family. They share a molecular domain organisation with the other intermediate filament proteins in that they are fibrous molecules that have an aminoterminal globular head, a central rod of alpha helices and a carboxy terminal globular domain. Many biochemical and molecular features of lamins have been studied, but their functions remain still largely undetermined. One of the functions ascribed to the lamina is the maintenance of the structural integrity of the nucleus. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
Lamin B1 polyclonal antibody detects endogenous levels of Lamin B1 protein. Immunogen :
Recombinant full length Human Lamin B1. Conjugate :
Unconjugated Modification :
Unmodification
Lamin B1 polyclonal antibody Background :
An important part of the nucleus is formed by nuclear lamina. Nuclear lamins form a network of filaments at the nucleoplasmic site of the nuclear membrane. Two main subtypes of nuclear lamins can be distinguished, i.e. A type lamins and B type lamins. The A type lamins comprise a set of three proteins arising from the same gene by alternative splicing, i.e. lamin A, lamin C and lamin Adel10, while the B-type lamins include two proteins arising from two distinct genes, i.e. lamin B1 and lamin B2. The nuclear lamins comprise a unique subclass of the intermediate filament protein family. They share a molecular domain organisation with the other intermediate filament proteins in that they are fibrous molecules that have an aminoterminal globular head, a central rod of alpha helices and a carboxy terminal globular domain. Many biochemical and molecular features of lamins have been studied, but their functions remain still largely undetermined. One of the functions ascribed to the lamina is the maintenance of the structural integrity of the nucleus. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
Lamin B1 polyclonal antibody detects endogenous levels of Lamin B1 protein. Immunogen :
Recombinant full length Human Lamin B1. Conjugate :
Unconjugated Modification :
Unmodification
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Western blot (WB) analysis of Lamin B1 polyclonal antibody at 1:20000 dilution Lane1:A549 whole cell lysate(40ug) Lane2:DLD whole cell lysate(40ug) Lane3:The Spleen tissue lysate of Mouse(40ug) Lane4:The Lung tissue lysate of Rat(40ug)
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Immunofluores image of AP6001 stained HEK293T cells. The cells were 4% paraformaldehyde fixed (20 min) and then incubated in 10% normal goat serum for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody Lamin B1 pAb #AP6001(1:200) at 5µg/ml overnight at +4°C. The secondary antibody (Red) was Goat Anti-Rabbit IgG (H+L) Rhodamine (TRITC) #BS10250 used at a 1/2000 dilution for 1h. DAPI #BD5014 was used to stain the cell nuclei (blue).
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Immunofluores image of AP6001 stained HEK293T cells. The cells were 4% paraformaldehyde fixed (20 min) and then incubated in 10% normal goat serum for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody Lamin B1 pAb #AP6001(1:200) at 5µg/ml overnight at +4°C. The secondary antibody (Red) was Goat Anti-Rabbit IgG (H+L) Rhodamine (TRITC) #BS10250 used at a 1/2000 dilution for 1h. DAPI #BD5014 was used to stain the cell nuclei (blue).
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Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.
Price/Size :
USD 368/1mg/vial
Tips:
For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).Describe :
Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.Formula:
Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.
Storage:
The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C.
Note :
This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.