Product Name :
β-Actin (D8) polyclonal antibody Background :
All eukaryotic cells express actin, which often constitutes asmuch as 50% of total cellular protein. Actin filaments can form both stable and labile structures and are crucial components of microvilli and the contractile apparatus of muscle cells. While lower eukaryotes, such as yeast, have only one actin gene, higher eukaryotes have several isoforms encoded by a family of genes. At least six types of actin are present in mammalian tissues and fall into three classes. -actin expression is limited to various types of muscle, whereas – and -actin are the principle constituents of filaments in other tissues.Members of the small GTPase family regulate the organization of the actin cytoskeleton. Rho controls the assembly of ctin stress fibers and focal adhesion, Rac regulates ctin filament accumulation at the plasma membrane and Cdc42 stimulates formation of filopodia. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
β-Actin (D8) polyclonal antibody detects endogenous levels of β-actin protein. Immunogen :
Recombinant full length Human β-Actin. Conjugate :
Unconjugated Modification :
Unmodification
β-Actin (D8) polyclonal antibody Background :
All eukaryotic cells express actin, which often constitutes asmuch as 50% of total cellular protein. Actin filaments can form both stable and labile structures and are crucial components of microvilli and the contractile apparatus of muscle cells. While lower eukaryotes, such as yeast, have only one actin gene, higher eukaryotes have several isoforms encoded by a family of genes. At least six types of actin are present in mammalian tissues and fall into three classes. -actin expression is limited to various types of muscle, whereas – and -actin are the principle constituents of filaments in other tissues.Members of the small GTPase family regulate the organization of the actin cytoskeleton. Rho controls the assembly of ctin stress fibers and focal adhesion, Rac regulates ctin filament accumulation at the plasma membrane and Cdc42 stimulates formation of filopodia. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
β-Actin (D8) polyclonal antibody detects endogenous levels of β-actin protein. Immunogen :
Recombinant full length Human β-Actin. Conjugate :
Unconjugated Modification :
Unmodification
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Western blot (WB) analysis of beta-actin (D8) polyclonal antibody at 1:5000 dilution Lane1:Hela cell lysate Lane2:sp2/0 cell lysate Lane3:PC12 cell lysate Western blot (WB) analysis of beta-actin (D8) polyclonal antibody at 1:10000 dilution Lane4:Hela cell lysate Lane5:sp2/0 cell lysate Lane6:PC12 cell lysate
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Roles of bovine viral diarrhea virus envelope glycoproteins in inducing autophagy in MDBK cells
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Dietary cholesterol promotes AOM-induced colorectal cancer through activating the NLRP3 inflammasome
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Roles of bovine viral diarrhea virus envelope glycoproteins in inducing autophagy in MDBK cells
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(S)-ZJM-289 Preconditioning Induces a Late Phase Protection Against Nervous Injury Induced by Transient Cerebral Ischemia and Oxygen-Glucose Deprivation
PMCID: Pubmed No.:24277159
Expression and Cell Distribution of Neuroglobin in the Brain Tissue After Experimental Subarachnoid Hemorrhage in Rats: A Pilot Study
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Dietary cholesterol promotes AOM-induced colorectal cancer through activating the NLRP3 inflammasome
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The Adverse Effect of Selective Cyclooxygenase-2 Inhibitor on Random Skin Flap Survival in Rats
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Dietary cholesterol promotes AOM-induced colorectal cancer through activating the NLRP3 inflammasome
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Lentivirus-mediated Bos taurus bta-miR-29b overexpression interferes with bovine viral diarrhoea virus replication and viral infection-related autophagy by directly targeting ATG14 and ATG9A in Madin–Darby bovine kidney cells
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Abnormal mRNA splicing but normal auditory brainstem response (ABR) in mice with the prestin (SLC26A5) IVS2-2A > G mutation
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A conditionally replicating adenovirus expressing IL-24 acts synergistically with temozolomide to enhance apoptosis in melanoma cells in vitro
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Chemopreventive activity of GEN-27, a genistein derivative, in colitis-associated cancer is mediated by p65-CDX2-β-catenin axis
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Cathelicidin‐related antimicrobial peptide protects against ischaemia reperfusion‐induced acute kidney injury in mice
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Rutin attenuates Sorafenib-induced Chemoresistance and Autophagy in Hepatocellular Carcinoma by regulating BANCR/miRNA-590-5P/OLR1 Axis
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Clemastine improves hypomyelination in rats with hypoxic–ischemic brain injury by reducing microglia-derived IL-1β via P38 signaling pathway
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Downregulation of Frizzled-7 induces the apoptosis of hepatocellular carcinoma cells through inhibition of NF-κB
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Luteolin inhibits angiogenesis of the M2‑like TAMs via the downregulation of hypoxia inducible factor‑1α and the STAT3 signalling pathway under hypoxia
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Neddylation inactivation affects cell cycle and apoptosis in sheep follicular granulosa cells
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Oncogenic TRIB2 interacts with and regulates PKM2 to promote aerobic glycolysis and lung cancer cell procession
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LncRNA-84277 is involved in chronic pain-related depressive behaviors through miR-128-3p/SIRT1 axis in central amygdala
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Semaglutide Protects against 6-OHDA Toxicity by Enhancing Autophagy and Inhibiting Oxidative Stress
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Effects of neuron autophagy induced by arsenic and fluoride on spatial learning and memory in offspring rats
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Effects of neuron autophagy induced by arsenic and fluoride on spatial learning and memory in offspring rats
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Effects of Compound Mycotoxin Detoxifier on Alleviating Aflatoxin B1-Induced Inflammatory Responses in Intestine, Liver and Kidney of Broilers
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Oridonin ameliorates caspase-9-mediated brain neuronal apoptosis in mouse with ischemic stroke by inhibiting RIPK3-mediated mitophagy
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β-catenin ISGylation promotes lipid deposition and apoptosis in ethanol-stimulated liver injury models
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CircRalgapa1 facilitates morphine tolerance via miR-873a-5p/A20 axis in mice
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FABP4-mediated lipid droplet formation in Streptococcus uberis-infected macrophages supports host defence
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Maternal circadian disruption before pregnancy impairs the ovarian function of female offspring in mice
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Halofuginone ameliorates systemic lupus erythematosus by targeting Blk in myeloid-derived suppressor cells
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SS31 Confers Cerebral Protection by Reversing Mitochondrial Dysfunction in Early Brain Injury Following Subarachnoid Hemorrhage, via the Nrf2- and PGC-1α-Dependent Pathways
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Mycobacterium tuberculosis PE_PGRS1 promotes mycobacteria intracellular survival via reducing the concentration of intracellular free Ca2+ and suppressing endoplasmic reticulum stress
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The functional link between TCTP and Drsl1 in deltamethrin-stressed Drosophila Kc cells
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Neuroprotective effect of hyperoside in MPP+/MPTP -induced dopaminergic neurodegeneration
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Small Molecule Compound K-7174 Attenuates Neuropsychiatric Manifestations in Lupus-prone Mice
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Increased Expression of the p-STAT3/IL-17 Signaling pathway in patients with Dermatomyositis
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Baicalin Nanocomplexes with an In Situ-Forming Biomimetic Gel Implant for Repair of Calvarial Bone Defects via Localized Sclerostin Inhibition
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The novel role of etoposide in inhibiting the migration and proliferation of small cell lung cancer and breast cancer via targeting Daam1
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MiR-146a-5p Contributes to Microglial Polarization Transitions Associated With AGEs
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Reduction of NADPH oxidase 4 in adipocytes contributes to the anti-obesity effect of dihydroartemisinin
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Imidazo[1,2-a]pyridine Derivatives as AMPK Activators: Synthesis, Structure–Activity Relationships, and Regulation of Reactive Oxygen Species in Renal Fibroblasts
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Delivery of miR-3529-3p using MnO2-SiO2-APTES nanoparticles combined with phototherapy suppresses lung adenocarcinoma progression by targeting HIGD1A
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Improved targeting delivery of WED-load immunoliposomes modified with SP-A mAb for the treatment of pulmonary fibrosis
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Wwl70-induced ABHD6 inhibition attenuates memory deficits and pathological phenotypes in APPswe/PS1dE9 mice
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Decidual macrophages derived NO downregulates PD-L1 in trophoblasts leading to decreased Treg cells in recurrent miscarriage
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A newly-synthesized compound CP-07 alleviates microglia-mediated neuroinflammation and ischemic brain injury via inhibiting STAT3 phosphorylation
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Caveolin-1 is essential for the increased release of glutamate in the anterior cingulate cortex in neuropathic pain mice
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Konjac glucomannan attenuate high-fat diet-fed obesity through enhancing β-adrenergic-mediated thermogenesis in inguinal white adipose tissue in mice
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Exo-miR-1290-induced by COX-2 overexpression promotes cancer-associated fibroblasts activation and tumor progression by CUL3-Nrf2 pathway in lung adenocarcinoma
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Targeting SDCBP2 in acute myeloid leukemia
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GSDME in Endothelial Cells: Inducing Vascular Inflammation and Atherosclerosis via Mitochondrial Damage and STING Pathway Activation
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Resveratrol ameliorates diabetic encephalopathy through PDE4D/PKA/Drp1 signaling
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Simvastatin ameliorates synaptic plasticity impairment in chronic mild stress-induced depressed mice by modulating hippocampal NMDA receptor
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Hippocampus protection from apoptosis by Baicalin in a LiCl-pilocarpine-induced rat status epilepticus model through autophagy activation
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Brassica oleracea L. extract ameliorates isoproterenol-induced myocardial injury by regulating HIF-1α-mediated glycolysis
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Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.
Price/Size :
USD 368/1mg/vial
Tips:
For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).Describe :
Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.Formula:
Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.
Storage:
The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C.
Note :
This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.