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β-Actin (D8) polyclonal antibody AP0731
  • Western blot (WB) analysis of beta-actin (D8) polyclonal antibody at 1:5000 dilution Lane1:Hela cell lysate Lane2:sp2/0 cell lysate Lane3:PC12 cell lysate Western blot (WB) analysis of beta-actin (D8) polyclonal antibody at 1:10000 dilution Lane4:Hela cell lysate Lane5:sp2/0 cell lysate Lane6:PC12 cell lysate
Product Nameβ-Actin (D8) polyclonal antibody
Catalog No.AP0731
Swiss-ProtN/A
Host Rabbit
ReactivityHuman,Mouse,Rat
ApplicationsWB
Application_allWB:1:5000~1:10000
BiowMW~ 42 kDa
Alternative NameActin, cytoplasmic 1; Beta-actin; Actin, cytoplasmic 1, N-terminally processed; ACTB; Actin-β
Purification&PurityThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
ConjugateUnconjugated
ModificationUnmodification
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50ul $148
100ul $258
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Product Name :
β-Actin (D8) polyclonal antibody
Background :
All eukaryotic cells express actin, which often constitutes asmuch as 50% of total cellular protein. Actin filaments can form both stable and labile structures and are crucial components of microvilli and the contractile apparatus of muscle cells. While lower eukaryotes, such as yeast, have only one actin gene, higher eukaryotes have several isoforms encoded by a family of genes. At least six types of actin are present in mammalian tissues and fall into three classes. -actin expression is limited to various types of muscle, whereas – and -actin are the principle constituents of filaments in other tissues.Members of the small GTPase family regulate the organization of the actin cytoskeleton. Rho controls the assembly of ctin stress fibers and focal adhesion, Rac regulates ctin filament accumulation at the plasma membrane and Cdc42 stimulates formation of filopodia.
Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity :
β-Actin (D8) polyclonal antibody detects endogenous levels of β-actin protein.
Immunogen :
Recombinant full length Human β-Actin.
Conjugate :
Unconjugated
Modification :
Unmodification
  • Western blot (WB) analysis of beta-actin (D8) polyclonal antibody at 1:5000 dilution Lane1:Hela cell lysate Lane2:sp2/0 cell lysate Lane3:PC12 cell lysate Western blot (WB) analysis of beta-actin (D8) polyclonal antibody at 1:10000 dilution Lane4:Hela cell lysate Lane5:sp2/0 cell lysate Lane6:PC12 cell lysate
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Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

Price/Size :

USD 368/1mg/vial



Tips: 

For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).

Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 


Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.
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