PPAR-γ (K160) polyclonal antibody 
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Product Name :
PPAR-γ (K160) polyclonal antibody Background :
PPAR gamma is implicated in numerous diseases including obesity, diabetes, atherosclerosis and cancer. PPAR gamma activators include prostanoids, fatty acids, thiazolidinediones and N-(2-benzoylphenyl) tyrosine analogues. A key component in adipocyte differentiation and fat-specific gene expression, PPAR gamma may modulate macrophage functions such as proinflammatory activities, and stimulate oxidized low-density lipoprotein (x-LDL) uptake. A Pro12Ala polymorphism of the PPAR gamma2 gene has been reported to reduce transactivation activity in vitro. This substitution may affect the immune response to ox-LDL and be associated with type 2 diabetes. In addition, the Pro12Ala variant of the PPAR gamma2 gene maybe correlated with abdominal obesity in type 2 diabetes. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
PPAR-γ (K160) polyclonal antibody detects endogenous levels of PPAR gamma protein. Immunogen :
Synthetic peptide, corresponding to amino acids 130-180 of Human PPAR-γ. Conjugate :
Unconjugated Modification :
Unmodification
PPAR-γ (K160) polyclonal antibody Background :
PPAR gamma is implicated in numerous diseases including obesity, diabetes, atherosclerosis and cancer. PPAR gamma activators include prostanoids, fatty acids, thiazolidinediones and N-(2-benzoylphenyl) tyrosine analogues. A key component in adipocyte differentiation and fat-specific gene expression, PPAR gamma may modulate macrophage functions such as proinflammatory activities, and stimulate oxidized low-density lipoprotein (x-LDL) uptake. A Pro12Ala polymorphism of the PPAR gamma2 gene has been reported to reduce transactivation activity in vitro. This substitution may affect the immune response to ox-LDL and be associated with type 2 diabetes. In addition, the Pro12Ala variant of the PPAR gamma2 gene maybe correlated with abdominal obesity in type 2 diabetes. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
PPAR-γ (K160) polyclonal antibody detects endogenous levels of PPAR gamma protein. Immunogen :
Synthetic peptide, corresponding to amino acids 130-180 of Human PPAR-γ. Conjugate :
Unconjugated Modification :
Unmodification
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Western blot (WB) analysis of PPAR-γ (K160) polyclonal antibody at 1:500 dilution Lane1:HEK293T whole cell lysate Lane2:Raw264.7 whole cell lysate Lane3:H9C2 whole cell lysate
Double antioxidant activities of rosiglitazone against high glucose-induced oxidative stress in hepatocyte
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The endocrine disruptor diethylstilbestrol induces adipocyte differentiation and promotes obesity in mice
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MicroRNA-130b and microRNA-374b mediate the effect of maternal dietary protein on offspring lipid metabolism in Meishan pigs
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Influence of Rosiglitazone on the Expression of PPARγ, NF-κB, and TNF-α in RatModel of Ulcerative Colitis
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Microvesicle-shuttled miR-130b reduces fat deposition in recipient primary cultured porcine adipocytes by inhibiting PPAR-γ expression
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Cerebroprotective effects of ibuprofen on diabetic encephalopathy in rats
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Effects of Caloric Intake on Learning and Memory Function in Juvenile C57BL/6J Mice
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14, 15‐epoxyeicosatrienoic acid promotes production of BDNF from astrocytes and exerts neuroprotective effects during ischemic injury
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Microvesicle-shuttled miR-130b reduces fat deposition in recipient primary cultured porcine adipocytes by inhibiting PPAR-γ expression
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Effects of Caloric Intake on Learning and Memory Function in Juvenile C57BL/6J Mice
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14, 15‐epoxyeicosatrienoic acid promotes production of BDNF from astrocytes and exerts neuroprotective effects during ischemic injury
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Cerebroprotective effects of ibuprofen on diabetic encephalopathy in rats
PMCID: Pubmed No.:24291733
Maternal butyrate supplementation induces insulin resistance associated with enhanced intramuscular fat deposition in the offspring
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ColXV promotes adipocyte differentiation via inhibiting DNA methylation and cAMP/PKA pathway in mice
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MicroRNA-130b attenuates dexamethasone-induced increase of lipid accumulation in porcine preadipocytes by suppressing PPAR-γ expression
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Maternal butyrate supplementation affects the lipid metabolism and fatty acid composition in the skeletal muscle of offspring piglets
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Hoxa5 alleviates obesity‐induced chronic inflammation by reducing ER stress and promoting M2 macrophage polarization in mouse adipose tissue
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MicroRNA-128 is involved in dexamethasone-induced lipid accumulation via repressing SIRT1 expression in cultured pig preadipocytes
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Hoxa5 Promotes Adipose Differentiation via Increasing DNA Methylation Level and Inhibiting PKA/HSL Signal Pathway in Mice
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Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.
Price/Size :
USD 368/1mg/vial
Tips:
For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).Describe :
Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.Formula:
Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.
Storage:
The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C.
Note :
This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.