Product Name :
GFP-tag (3A10) monoclonal antibody Background :
Green fluorescent protein (GFP) is a 27 a protein derived from the jellyfish Aequorea victoria, which emits green light (emission peak at a wavelength of 509 nm) when excited by blue light (excitation peak at a wavelength of 395 nm). GFP has become an invaluable tool in cell biology research, since its intrinsic fluorescence can be visualized in living cells. GFP fluorescence is stable under fixation conditions and suitable for a variety of applications. GFP has been widely used as a reporter for gene expression, enabling researchers to visualize and localize GFP-tagged proteins within living cells without the need for chemical staining. Other applications of GFP include assessment of protein protein interactions through the yeast two hybrid system and measurement of distance between proteins through fluorescence energy transfer (FRET) protocols. Product :
1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
GFP-tag (3A10) mAb detects over-expressed or recombinant proteins containing the GFP epitope tag. Immunogen :
Synthetic peptide GFP-tag. Conjugate :
Unconjugated Modification :
Unmodification

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  Western blot (WB) analysis of GFP-tag (3A10) mAb-HRP at 1:2000/1:5000/1:10000 dilution Lane1:HEK293T whole cell lysate Lane2:HEK293T whole cell lysate,transfected with pEGFP-C3-ATG3. Lane3:HEK293T whole cell lysate,transfected with pEGFP-C3-ATG12.
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  Immunoprecipitation of HEK293T,transfected with pEGFP-C3-ATG3#PPL00361-2a, cell lysates using GFP-tag (3A10) mAb (Sepharose Bead Conjugate)#BD0047 (lane 2)and Nonspecific IgG Control (Sepharose Bead Conjugate)#BD0047 (lane 3) .Lane 1 is 30% input. The western blot was probed using GFP-tag (3A10) mAb#AP0675M.“↑”(supernatant); “↓”(deposition)

Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

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Price/Size :

USD 368/1mg/vial

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Tips: 

For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).

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Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

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Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

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Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 

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Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.

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