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MRP8 polyclonal antibody AP0476
  • Western blot (WB) analysis of MRP8 pAb at 1:500 dilution Lane1:PC12 whole cell lysate(40ug) Lane2:SP2/0 whole cell lysate(40ug) Lane3:EC9706 whole cell lysate(40ug) Lane4:A375 whole cell lysate(40ug) Lane5:HCC827 whole cell lysate(40ug)
  • Immunohistochemistry (IHC) analyzes of MRP8 pAb in paraffin-embedded human breast carcinoma tissue at 1:100.
Product NameMRP8 polyclonal antibody
Catalog No.AP0476
Swiss-ProtP05109
Host Rabbit
ReactivityHuman,Mouse,Rat
ApplicationsWB IHC
Application_allWB:1:500~1:1000 IHC:1:50~1:200
BiowMW~ 23 kDa
Alternative NameProtein S100-A8; Calgranulin-A; Calprotectin L1L subunit; Cystic fibrosis antigen; CFAG; Leukocyte L1 complex light chain; Migration inhibitory factor-related protein 8; MRP-8; S100 calcium-binding protein A8; Urinary stone protein band A; Protein S100-A8, N-terminally processed; S100A8; CAGA; CFAG; MRP8
Purification&PurityThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 101% (by SDS-PAGE).
ConjugateUnconjugated
ModificationUnmodification
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Size Price
50ul $158
100ul $275
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Product Name :
MRP8 polyclonal antibody
Background :
The family of EF-hand type Ca2+-binding proteins includes Calbindin (previously designated vitamin D-dependent Ca2+-binding protein), S-100 α and β, Cal-granulins A (also designated MRP8), B (also designated MRP14) and C (S-100 like proteins) and the parvalbumin family members, including parvalbumin α and parvalbumin β (also designated oncomodulin). Calbindin, S-100 proteins and parvalbumin proteins are each expressed in neural tissues. In addition, S-100 α and β are present in a variety of other tissues and Calbindin is present in intestine and kidney. Parvalbumin α is also found in fast-contracting/ relaxing skeletal muscle fibers and parvalbumin β is found in many tumor tissues as well as in the organ of Corti.
Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity :
MRP8 polyclonal antibody detects endogenous levels of MRP8 protein.
Immunogen :
Synthetic peptide, corresponding to amino acids CMGVAAHKKSHEESHKE of Human MRP8.
Conjugate :
Unconjugated
Modification :
Unmodification
  • Western blot (WB) analysis of MRP8 pAb at 1:500 dilution Lane1:PC12 whole cell lysate(40ug) Lane2:SP2/0 whole cell lysate(40ug) Lane3:EC9706 whole cell lysate(40ug) Lane4:A375 whole cell lysate(40ug) Lane5:HCC827 whole cell lysate(40ug)
  • Immunohistochemistry (IHC) analyzes of MRP8 pAb in paraffin-embedded human breast carcinoma tissue at 1:100.
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

Price/Size :

USD 368/1mg/vial



Tips: 

For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).

Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 


Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.
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