HSP27 polyclonal antibody 
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Product Name :
HSP27 polyclonal antibody Background :
Hsp27, also referred to as the Estrogen-Regulated 24K protein and HSP28, is one of several small heat shock proteins (HSP) produced by all organisms studied. Hsp27 synthesis is induced by elevated temperature, as well as estrogen in hormone responsive cells. This protein is involved in stress resistance and actin organization. Interestingly, human HSP27 also shares greater than 50% homology with low molecular weight Drosophila HSP's and mammalian a-crystalline lens protein. Because of the estrogen-responsive nature of Hsp27, this protein has been studied extensively in human estrogen-responsive tissues such as cervix, endometrium and breast tissue. This work has led to the suggestion that Hsp27 may be a useful marker in classifying various hormone sensitive tumors. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
HSP27 polyclonal antibody detects endogenous levels of HSP27 protein. Immunogen :
Synthetic peptide, corresponding to amino acids 50-100 of Human HSP27. Conjugate :
Unconjugated Modification :
Unmodification
HSP27 polyclonal antibody Background :
Hsp27, also referred to as the Estrogen-Regulated 24K protein and HSP28, is one of several small heat shock proteins (HSP) produced by all organisms studied. Hsp27 synthesis is induced by elevated temperature, as well as estrogen in hormone responsive cells. This protein is involved in stress resistance and actin organization. Interestingly, human HSP27 also shares greater than 50% homology with low molecular weight Drosophila HSP's and mammalian a-crystalline lens protein. Because of the estrogen-responsive nature of Hsp27, this protein has been studied extensively in human estrogen-responsive tissues such as cervix, endometrium and breast tissue. This work has led to the suggestion that Hsp27 may be a useful marker in classifying various hormone sensitive tumors. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
HSP27 polyclonal antibody detects endogenous levels of HSP27 protein. Immunogen :
Synthetic peptide, corresponding to amino acids 50-100 of Human HSP27. Conjugate :
Unconjugated Modification :
Unmodification
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Western blot (WB) analysis of HSP27 polyclonal antibody at 1:500 dilution Lane1:Hela whole cell lysate(40ug) Lane2:H9C2 whole cell lysate(40ug) Lane3:PC12 whole cell lysate(40ug) -
Western blot (WB) analysis of HSP27 (S78) pAb at 1:500 dilution Lane1:Hela whole cell lysate(40ug) Lane2:H9C2 whole cell lysate(40ug) Lane3:PC12 whole cell lysate(40ug) -
Western blot (WB) analysis of HSP27 (S78) pAb at 1:500 dilution Lane1:Hela whole cell lysate(40ug) Lane2:H9C2 whole cell lysate(40ug) Lane3:PC12 whole cell lysate(40ug)
PP2A-AMPKα-HSF1 axis regulates metal-induced HSPs expression and ROS clearance
PMCID: Pubmed No.:24412756
Proteomic Analysis of Cellular Response Induced by Multi-Walled Carbon Nanotubes Exposure in A549 Cells
PMCID: Pubmed No.:24454774
Identification of a< i> Bombyx mori gene encoding small heat shock protein BmHsp27. 4 expressed in response to high-temperature stress
PMCID: Pubmed No.:24440242
Proteomic analysis of sheep primary testicular cells infected with bluetongue virus
PMCID: Pubmed No.:26989863
Proteomic analysis of sheep primary testicular cells infected with bluetongue virus
PMCID: Pubmed No.:26989863
PP2A-AMPKα-HSF1 axis regulates metal-induced HSPs expression and ROS clearance
PMCID: Pubmed No.:24412756
Proteomic Analysis of Cellular Response Induced by Multi-Walled Carbon Nanotubes Exposure in A549 Cells
PMCID: Pubmed No.:24454774
Identification of a< i> Bombyx mori gene encoding small heat shock protein BmHsp27. 4 expressed in response to high-temperature stress
PMCID: Pubmed No.:24440242
Phosphorylated Hsp27 promotes adriamycin resistance in breast cancer cells through regulating dual phosphorylation of c-Myc
PMCID: Pubmed No.:37797796
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.
Price/Size :
USD 368/1mg/vial
Tips:
For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).Describe :
Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.Formula:
Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.
Storage:
The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C.
Note :
This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.