HSP27 (phospho-S15) polyclonal antibody 
Datasheet
COA
MSDS
SHIP
Product Name :
HSP27 (phospho-S15) polyclonal antibody Background :
Hsp27, also referred to as the Estrogen-Regulated 24K protein and HSP28, is one of several small heat shock proteins (HSP) produced by all organisms studied. Hsp27 synthesis is induced by elevated temperature, as well as estrogen in hormone responsive cells. This protein is involved in stress resistance and actin organization. Interestingly, human HSP27 also shares greater than 50% homology with low molecular weight Drosophila HSP's and mammalian a-crystalline lens protein. Because of the estrogen-responsive nature of Hsp27, this protein has been studied extensively in human estrogen-responsive tissues such as cervix, endometrium and breast tissue. This work has led to the suggestion that Hsp27 may be a useful marker in classifying various hormone sensitive tumors. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
HSP27 (phospho-S15) polyclonal antibody detects endogenous levels of HSP27 protein only when phosphorylated at Ser15 Immunogen :
Synthetic phosphopeptide derived from human HSP27 around the phosphorylation site of Serine 15. Conjugate :
Unconjugated Modification :
Phosphorylation
HSP27 (phospho-S15) polyclonal antibody Background :
Hsp27, also referred to as the Estrogen-Regulated 24K protein and HSP28, is one of several small heat shock proteins (HSP) produced by all organisms studied. Hsp27 synthesis is induced by elevated temperature, as well as estrogen in hormone responsive cells. This protein is involved in stress resistance and actin organization. Interestingly, human HSP27 also shares greater than 50% homology with low molecular weight Drosophila HSP's and mammalian a-crystalline lens protein. Because of the estrogen-responsive nature of Hsp27, this protein has been studied extensively in human estrogen-responsive tissues such as cervix, endometrium and breast tissue. This work has led to the suggestion that Hsp27 may be a useful marker in classifying various hormone sensitive tumors. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
HSP27 (phospho-S15) polyclonal antibody detects endogenous levels of HSP27 protein only when phosphorylated at Ser15 Immunogen :
Synthetic phosphopeptide derived from human HSP27 around the phosphorylation site of Serine 15. Conjugate :
Unconjugated Modification :
Phosphorylation
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Western blot (WB) analysis of HSP27 (phospho-S15) pAb at 1:500 dilution Lane1:L02 whole cell lysate Lane2:SGC7901 whole cell lysate Lane3:HEK293T transfected with pCDNA3.1-Flag-HSP27 whole cell lysate Lane4:HEK293T whole cell lysate -
Immunoprecipitation of L02 whole cell lysate using HSP27 (phospho-S15) pAb (Sepharose Bead Conjugate) #BD0047(lane 2 and lane 3) and Nonspecific IgG Control (Sepharose Bead Conjugate)#BD0047 (lane 4) .Lane 1 is 20% input. The western blot was probed using HSP27 (phospho-S15) pAb #AP0245.“↑”（supernatant）; “↓”（deposition）
Microcystin-LR induces cytoskeleton system reorganization through hyperphosphorylation of tau and HSP27 via PP2A inhibition and subsequent activation of the p38 MAPK signaling pathway in neuroendocrine (PC12) cells.
PMCID: Pubmed No.:22000993
Phosphorylation of heat shock protein 27 antagonizes TNF-α induced HeLa cell apoptosis via regulating TAK1 ubiquitination and activation of p38 and ERK signaling.
PMCID: Pubmed No.:24686082
Crosstalk between JNK and NF-κB signaling pathways via HSP27 phosphorylation in HepG2 cells
PMCID: Pubmed No.:25446109
Heat shock protein 27 phosphorylation in the proliferation and apoptosis of human umbilical vein endothelial cells induced by high glucose through the phosphoinositide 3‑kinase/Akt and extracellular signal‑regulated kinase 1/2 pathways.
PMCID: Pubmed No.:25373458
Plasma miR-185 is decreased in esophageal squamous cell carcinoma patients and might suppress tumor migration and invasion by targeting RAGE.
PMCID: Pubmed No.:26316588
Crosstalk between JNK and NF-κB signaling pathways via HSP27 phosphorylation in HepG2 cells
PMCID: Pubmed No.:25446109
Heat shock protein 27 phosphorylation in the proliferation and apoptosis of human umbilical vein endothelial cells induced by high glucose through the phosphoinositide 3‑kinase/Akt and extracellular signal‑regulated kinase 1/2 pathways.
PMCID: Pubmed No.:25373458
Phosphorylation of heat shock protein 27 antagonizes TNF-α induced HeLa cell apoptosis via regulating TAK1 ubiquitination and activation of p38 and ERK signaling.
PMCID: Pubmed No.:24686082
Phosphorylated Hsp27 promotes adriamycin resistance in breast cancer cells through regulating dual phosphorylation of c-Myc
PMCID: Pubmed No.:37797796
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.
Price/Size :
USD 368/1mg/vial
Tips:
For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).Describe :
Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.Formula:
Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.
Storage:
The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C.
Note :
This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.