CXCR4 (phospho-S339) polyclonal antibody 
Datasheet
COA
MSDS
SHIP
Product Name :
CXCR4 (phospho-S339) polyclonal antibody Background :
The C-X-C or α chemokine family is characterized by a pair of cysteine residues separated by a single amino acid and primarily functions as chemoattractants for neutrophils. The C-X-C family includes IL-8, NAP-2, MSGA and stromal cell derived factor-1 or SDF-1. SDF-1 was originally described as a pre-B cell stimulatory factor, but has now been shown to function as a potent chemoattractant for T cells and monocytes but not neutrophils. Receptors for the C-X-C family are G protein coupled, seven pass transmembrane domain proteins which include IL-8RA, IL-8RB and fusin (variously referred to as LESTR or CXCR-4). Fusin is highly homologous to the IL-8 receptors, sharing 37% sequence identity at the amino acid level. The IL-8 receptors bind to IL-8, NAP-2 and MSGA, while fusin binds to its cognate ligand, SDF-1. Fusin has been identified as the major coreceptor for T-tropic HIV-1 and SDF-1 has been shown to inhibit HIV-1 infection. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
p-CXCR4 (S339) polyclonal antibody detects endogenous levels of CXCR4 protein only when phosphorylated at Ser339. Immunogen :
Synthetic phosphopeptide derived from human CXCR4 around the phosphorylation site of Serine 339. Conjugate :
Unconjugated Modification :
Phosphorylation
CXCR4 (phospho-S339) polyclonal antibody Background :
The C-X-C or α chemokine family is characterized by a pair of cysteine residues separated by a single amino acid and primarily functions as chemoattractants for neutrophils. The C-X-C family includes IL-8, NAP-2, MSGA and stromal cell derived factor-1 or SDF-1. SDF-1 was originally described as a pre-B cell stimulatory factor, but has now been shown to function as a potent chemoattractant for T cells and monocytes but not neutrophils. Receptors for the C-X-C family are G protein coupled, seven pass transmembrane domain proteins which include IL-8RA, IL-8RB and fusin (variously referred to as LESTR or CXCR-4). Fusin is highly homologous to the IL-8 receptors, sharing 37% sequence identity at the amino acid level. The IL-8 receptors bind to IL-8, NAP-2 and MSGA, while fusin binds to its cognate ligand, SDF-1. Fusin has been identified as the major coreceptor for T-tropic HIV-1 and SDF-1 has been shown to inhibit HIV-1 infection. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
p-CXCR4 (S339) polyclonal antibody detects endogenous levels of CXCR4 protein only when phosphorylated at Ser339. Immunogen :
Synthetic phosphopeptide derived from human CXCR4 around the phosphorylation site of Serine 339. Conjugate :
Unconjugated Modification :
Phosphorylation
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Western blot (WB) analysis of p-CXCR4 (S339) pAb at 1:500 dilution Lane1:Myla2059 whole cell lysate(40ug) Lane2:K562 whole cell lysate(40ug) Lane3:Jurkat whole cell lysate(40ug)
Specific chemotherapeutic agents induce metastatic behaviour through stromal‐ and tumour‐derived cytokine and angiogenic factor signalling
PMCID: Pubmed No.:25988668
CXCL12/CXCR4 Chemokine Signaling in Spinal Glia Induces Pain Hypersensitivity through MAPKs‐mediated Neuroinflammation in Bone Cancer Rats
PMCID: Pubmed No.:25393328
CXCL12/CXCR4 Chemokine Signaling in Spinal Glia Induces Pain Hypersensitivity through MAPKs‐mediated Neuroinflammation in Bone Cancer Rats
PMCID: Pubmed No.:25393328
Retracted: CXCL12/CXCR4 chemokine signaling in spinal glia induces pain hypersensitivity through MAPKs‐mediated neuroinflammation in bone cancer rats
PMCID: Pubmed No.:25393328
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.
Price/Size :
USD 368/1mg/vial
Tips:
For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).Describe :
Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.Formula:
Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.
Storage:
The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C.
Note :
This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.