Product Name :
HBO1 (S167) polyclonal antibody Background :
KAT7 / Hbo1 is a member of the MYST histone acetyltransferae family which acetylates both histone H3 and H4 and is involved in the regulation of replication. The initiation of DNA replication is tightly regulated in eukaryotic cells to ensure that the genome is precisely duplicated once per cell cycle. This is accomplished by controlling the pre-replicative complex. KAT7 acts as a positive regulatory factor for pre-RC assembly and thus controls DNA replication. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
HBO1 (S167) polyclonal antibody detects endogenous levels of HBO1 protein. Immunogen :
Synthetic peptide, corresponding to amino acids 141-190 of Human HBO1. Conjugate :
Unconjugated Modification :
Unmodification

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  Western blot (WB) analysis of HBO1 (S167) polyclonal antibody at 1:500 dilution Lane1:HepG2 whole cell lysate(40ug) Lane2:A549 whole cell lysate(40ug) Lane3:HEK293T whole cell lysate(40ug) Lane4:PC12 whole cell lysate(40ug) Lane5:AML-12 whole cell lysate(40ug)
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Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

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Price/Size :

USD 368/1mg/vial

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Tips: 

For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).

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Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

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Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

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Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 

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Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.

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