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Nrf2 (L593) polyclonal antibody BS1258
  • Western blot (WB) analysis of Nrf2 (L593) pAb at 1:500 dilution Lane1:AML-12 whole cell lysate(40ug) Lane2:PC12 whole cell lysate(40ug) Lane3:Myla2059 whole cell lysate(40ug) Lane4:SGC7901 whole cell lysate(40ug) Lane5:Hela whole cell lysate(40ug)
  • Immunohistochemistry (IHC) analyzes of Nrf2 (L593) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.
Product NameNrf2 (L593) polyclonal antibody
Catalog No.BS1258
Swiss-ProtQ16236
Host Rabbit
ReactivityHuman,Mouse,Rat
ApplicationsWB IHC
Application_allWB: 1:500~1:1000_x000D_ IHC: 1:50~1:200
BiowMW~ 100 kDa
Alternative NameNuclear factor erythroid 2-related factor 2; NF-E2-related factor 2; NFE2-related factor 2; HEBP1; NFE2L2; NRF2
Purification&PurityThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
ConjugateUnconjugated
ModificationUnmodification
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50ul $158
100ul $275
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Product Name :
Nrf2 (L593) polyclonal antibody
Background :
Nuclear factor erythroid 2-related factor 2 (Nrf2) is a transcription factor which regulates the expression of many detoxification and antioxidant enzymes. Nrf2 can potentially play a significant role in adaptive responses to oxidative stress. Nrf2 belongs to the Cap N Collar (CNC-bZIP subfamily of basic /leucine zipper (bZIP) transcription factors.
Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity :
Nrf2/NFE2L2 (L593) polyclonal antibody detects endogenous levels of Nrf2/NFE2L2 protein.
Immunogen :
Synthetic peptide, corresponding to amino acids 560-621 of Human Nrf2.
Conjugate :
Unconjugated
Modification :
Unmodification
  • Western blot (WB) analysis of Nrf2 (L593) pAb at 1:500 dilution Lane1:AML-12 whole cell lysate(40ug) Lane2:PC12 whole cell lysate(40ug) Lane3:Myla2059 whole cell lysate(40ug) Lane4:SGC7901 whole cell lysate(40ug) Lane5:Hela whole cell lysate(40ug)
  • Immunohistochemistry (IHC) analyzes of Nrf2 (L593) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.
PGD2 and PGE2 regulate gene expression of Prx 6 in primary macrophages via Nrf2 PMCID:    Pubmed No.:21651978 Cardioprotective Effect of Paeonol and Danshensu Combination on Isoproterenol-Induced Myocardial Injury in Rats PMCID:    Pubmed No.:23139821 3,3′-Diindolylmethane Protects against Cardiac Hypertrophy via 5′-Adenosine Monophosphate-Activated Protein Kinase-α2 PMCID:    Pubmed No.:23326427 The Protective Effect of Alpha-Lipoic Acid in Lipopolysaccharide-Induced Acute Lung Injury Is Mediated by Heme Oxygenase-1 PMCID:    Pubmed No.:23573137 Olmesartan ameliorates urinary dysfunction in the spontaneously hypertensive rat via recovering bladder blood flow and decreasing oxidative stress PMCID:    Pubmed No.:23775684 Regulation of antioxidant system, lipids and fatty acid β-oxidation contributes to the cardioprotective effect of sodium tanshinone IIA sulphonate in isoproterenol-induced myocardial infarction in rats PMCID:    Pubmed No.:23958267 Sodium hydrosulfide alleviates lung inflammation and cell apoptosis following resuscitated hemorrhagic shock in rats PMCID:    Pubmed No.:24122010 (S)-ZJM-289 Preconditioning Induces a Late Phase Protection Against Nervous Injury Induced by Transient Cerebral Ischemia and Oxygen-Glucose Deprivation PMCID:    Pubmed No.:24277159 Attenuation of Cigarette Smoke-Induced Airway Mucus Production by Hydrogen-Rich Saline in Rats PMCID:    Pubmed No.:24376700 Oxymatrine prevents hypoxia- and monocrotaline-induced pulmonary hypertension in rats PMCID:    Pubmed No.:24440469 Baicalein, an active component of< i> Scutellaria baicalensis, protects against lipopolysaccharide-induced acute lung injury in rats PMCID:    Pubmed No.:24534526 Protective Effects of BML-111 on Cerulein-Induced Acute Pancreatitis-Associated Lung Injury via Activation of Nrf2/ARE Signaling Pathway PMCID:    Pubmed No.:24550037 Wogonin-enhanced reactive oxygen species-induced apoptosis and potentiated cytotoxic effects of chemotherapeutic agents by suppression Nrf2-mediated signaling in HepG2 cells. PMCID:    Pubmed No.:24666416 Long-term blue light exposure induces RGC-5 cell death in vitro: involvement of mitochondria-dependent apoptosis, oxidative stress, and MAPK signaling pathways PMCID:    Pubmed No.:24682673 Oroxylin A exerts anti-inflammatory activity on lipopolysaccharide-induced mouse macrophage via Nrf2/ARE activation. PMCID:    Pubmed No.:25247252 Dichlorodiphenyldichloroethylene exposure reduces r‐GCS via suppressed Nrf2 in HepG2 cells PMCID:    Pubmed No.:25263748 Reduction of DNA damage induced by titanium dioxide nanoparticles through Nrf2 in vitro and in vivo PMCID:    Pubmed No.:26091733 Histone deacetylase inhibition activates Nrf2 and protects against osteoarthritis PMCID:    Pubmed No.:26408027 An integrated pathway interaction network for the combination of four effective compounds from ShengMai preparations in the treatment of cardio-cerebral ischemic diseases. PMCID:    Pubmed No.:26456587 3-aroylmethylene-2,3,6,7-tetrahydro-1H-pyrazino[2,1-a]isoquinolin-4(11bH)-ones as potent Nrf2/ARE inducers in human cancer cells and AOM-DSS treated mice. PMCID:    Pubmed No.:24053646 Protective effects of alisol B 23-acetate from edible botanical Rhizoma alismatis against carbon tetrachloride-induced hepatotoxicity in mice PMCID:    Pubmed No.:25747392 Genomic and proteomic analysis of the inhibition of synthesis and secretion of aldosterone hormone induced by quinocetone in NCI-H295R cells PMCID:    Pubmed No.:27046791 Oroxylin A exerts anti-inflammatory activity on lipopolysaccharide-induced mouse macrophage via Nrf2/ARE activation. PMCID:    Pubmed No.:25247252 Dichlorodiphenyldichloroethylene exposure reduces r‐GCS via suppressed Nrf2 in HepG2 cells PMCID:    Pubmed No.:25263748 Sodium hydrosulfide alleviates lung inflammation and cell apoptosis following resuscitated hemorrhagic shock in rats PMCID:    Pubmed No.:24122010 (S)-ZJM-289 Preconditioning Induces a Late Phase Protection Against Nervous Injury Induced by Transient Cerebral Ischemia and Oxygen-Glucose Deprivation PMCID:    Pubmed No.:24277159 Histone deacetylase inhibition activates Nrf2 and protects against osteoarthritis PMCID:    Pubmed No.:26408027 An integrated pathway interaction network for the combination of four effective compounds from ShengMai preparations in the treatment of cardio-cerebral ischemic diseases. PMCID:    Pubmed No.:26456587 3-aroylmethylene-2,3,6,7-tetrahydro-1H-pyrazino[2,1-a]isoquinolin-4(11bH)-ones as potent Nrf2/ARE inducers in human cancer cells and AOM-DSS treated mice. PMCID:    Pubmed No.:24053646 Protective effects of alisol B 23-acetate from edible botanical Rhizoma alismatis against carbon tetrachloride-induced hepatotoxicity in mice PMCID:    Pubmed No.:25747392 Genomic and proteomic analysis of the inhibition of synthesis and secretion of aldosterone hormone induced by quinocetone in NCI-H295R cells PMCID:    Pubmed No.:27046791 Attenuation of Cigarette Smoke-Induced Airway Mucus Production by Hydrogen-Rich Saline in Rats PMCID:    Pubmed No.:24376700 Oxymatrine prevents hypoxia- and monocrotaline-induced pulmonary hypertension in rats PMCID:    Pubmed No.:24440469 Baicalein, an active component of< i> Scutellaria baicalensis, protects against lipopolysaccharide-induced acute lung injury in rats PMCID:    Pubmed No.:24534526 Protective Effects of BML-111 on Cerulein-Induced Acute Pancreatitis-Associated Lung Injury via Activation of Nrf2/ARE Signaling Pathway PMCID:    Pubmed No.:24550037 Wogonin-enhanced reactive oxygen species-induced apoptosis and potentiated cytotoxic effects of chemotherapeutic agents by suppression Nrf2-mediated signaling in HepG2 cells. PMCID:    Pubmed No.:24666416 Long-term blue light exposure induces RGC-5 cell death in vitro: involvement of mitochondria-dependent apoptosis, oxidative stress, and MAPK signaling pathways PMCID:    Pubmed No.:24682673 Heme Oxygenase-1 and Carbon Monoxide Promote Burkholderia pseudomallei Infection PMCID:    Pubmed No.:27316684 Piceatannol inhibits the IL-1β-induced inflammatory response in human osteoarthritic chondrocytes and ameliorates osteoarthritis in mice by activating Nrf2 PMCID:    Pubmed No.:28933476 Wogonin reversed resistant human myelogenous leukemia cells via inhibiting Nrf2 signaling by Stat3/NF-κB inactivation PMCID:    Pubmed No.:28150717 Lipopolysaccharide–induced preconditioning protects against traumatic spinal cord injury by upregulating Nrf2 expression in rats PMCID:    Pubmed No.:27515502 Reduction of oxidative damages induced by titanium dioxide nanoparticles correlates with induction of the Nrf2 pathway by GSPE supplementation in mice PMCID:    Pubmed No.:28780322 Synthesis and cytotoxicity evaluation of 3-amino-2-hydroxypropoxyisoflavone derivatives PMCID:    Pubmed No.:27473965 Protective effect of melatonin on myenteric neuron damage in experimental colitis in rats PMCID:    Pubmed No.:26787455 Deletion of Jdp2 enhances Slc7a11 expression in Atoh-1 positive cerebellum granule cell progenitors in vivo PMCID:    Pubmed No.:34187574 Protective Effects of Pterostilbene on Lipopolysaccharide-Induced Acute Lung Injury in Mice by Inhibiting NF-κB and Activating Nrf2/HO-1 Signaling Pathways. PMCID:    Pubmed No.:33633565 Neuroprotective Effects of Shenqi Fuzheng Injection in a Transgenic SOD1-G93A Mouse Model of Amyotrophic Lateral Sclerosis PMCID:    Pubmed No.:34737697 LL202 ameliorates colitis against oxidative stress of macrophage by activation of the Nrf2/HO‐1 pathway PMCID:    Pubmed No.:30426485 β-Hydroxyisovalerylshikonin inhibits IL-1β-induced chondrocyte inflammation via Nrf2 and retards osteoarthritis in mice PMCID:    Pubmed No.:33169745 Targeting PI3K/Akt/Nrf2 pathway by glabridin alleviates acetaminophen-induced hepatic injury in rats PMCID:    Pubmed No.:33932887 3-(1H-Benzo[d]imidazol-6-yl)-5-(4-fluorophenyl)-1,2,4-oxadiazole (DDO7232), a Novel Potent Nrf2/ARE Inducer, Ameliorates DSS-Induced Murine Colitis and Protects NCM460 Cells against Oxidative Stress via ERK1/2 Phosphorylation PMCID:    Pubmed No.:29887940 Ginseng root extract attenuates inflammation by inhibiting the MAPK/NF-κB signaling pathway and activating autophagy and p62-Nrf2-Keap1 signaling in vitro and in vivo PMCID:    Pubmed No.:34648903 Sodium butyrate protects against high-fat diet-induced oxidative stress in rat liver by promoting expression of nuclear factor E2-related factor 2 PMCID:    Pubmed No.:31204637 1,25-Dihydroxyvitamin D insufficiency accelerates age-related bone loss by increasing oxidative stress and cell senescence PMCID:    Pubmed No.:32194899 Genistein attenuates di‑(2‑ethylhexyl) phthalate-induced testicular injuries via activation of Nrf2/HO‑1 following prepubertal exposure PMCID:    Pubmed No.:29328408 Ameliorative effects and mechanisms of salvianic acid A on retinal iron overload in vivo and in vitro PMCID:    Pubmed No.:34058232 7,8‑Dihydroxyflavone activates Nrf2/HO‑1 signaling pathways and protects against osteoarthritis PMCID:    Pubmed No.:31410125 Preparation of baicalin-loaded ligand-modified nanoparticles for nose-to-brain delivery for neuroprotection in cerebral ischemia PMCID:    Pubmed No.:35467483 Neuroprotective effect of Ginsenoside Re against neurotoxin‑induced Parkinson's disease models via induction of Nrf2 PMCID:    Pubmed No.:35543148 Ubiquitin-specific protease 7 regulates myocardial ischemia/reperfusion injury by stabilizing Keap1 PMCID:    Pubmed No.:35710902 Mesenchymal stem cells-derived small extracellular vesicles alleviate diabetic retinopathy by delivering NEDD4 PMCID:    Pubmed No.:35841055 Probiotic Pediococcus pentosaceus ameliorates MPTP-induced oxidative stress via regulating the gut microbiota–gut–brain axis PMCID:    Pubmed No.:36439235 Effects of CEACAM1 in oral keratinocytes on HO-1 expression induced by Candida β-glucan particles PMCID:    Pubmed No.:36350873 N6-methyladenosine mediates Nrf2 protein expression involved in PM2.5-induced pulmonary fibrosis PMCID:    Pubmed No.:36917877
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

Price/Size :

USD 368/1mg/vial



Tips: 

For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).

Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 


Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.
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