Product Name :
ATP5H (D146) polyclonal antibody Background :
ATP5H (ATP synthase, H+ transporting, mitochondrial Fo complex, subunit δ), also known as ATPQ, is a 161 amino acid protein that belongs to the ATPase δ subunit family. F-type ATPases, such as ATP5H, consist of two linked components: CF1, a soluble catalytic core that consists of five different subunits (α, β, γ, δ and ε), and CF0, a membrane proton channel that contains nine subunits (α, β, χ, δ, ε, φ, γ, F6 and 8). ATP5H encodes the δ subunit of the F0 complex. ATP5H produces ATP from ADP in the presence of a proton gradient across the membrane, which is generated by electron transport complexes of the respiratory chain. Localizing to mitochondrial inner membrane, Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
ATP5H (D146) polyclonal antibody detects endogenous levels of ATP5H protein. Immunogen :
Synthetic peptide, corresponding to amino acids 121-170 of Human ATP5H. Conjugate :
Unconjugated Modification :
Unmodification

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  Western blot (WB) analysis of ATP5H (D146) pAb at 1:500 dilution Lane1:Hela whole cell lysate(40ug) Lane2:MCF-7 whole cell lysate(40ug) Lane3:H1792 whole cell lysate(40ug) Lane4:C6 whole cell lysate(40ug) Lane5:AML-12 whole cell lysate(40ug)
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  Immunohistochemistry (IHC) analyzes of ATP5H (D146) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.
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  Immunohistochemistry (IHC) analyzes of ATP5H (D146) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.

Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

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Price/Size :

USD 368/1mg/vial

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Tips: 

For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).

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Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

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Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

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Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 

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Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.

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