Product Name :
Factor IX HC (V227) polyclonal antibody Background :
Hemostasis following tissue injury involves the deployment of essential plasma procoagulants (Prothrombin and factors X, IX, V and VIII), which are involved in a blood coagulation cascade that leads to the formation of insoluble Fibrin clots and the promotion of platelet aggregation. Coagulation Factor IX (plasma thromboplastic component, F9, F.IX, HEMB) is a vitamin Kdependent, single chain serine protease that is synthesized in the liver and circulates as an inactive precursor. Factor XI A mediated proteolytic cleavage of Factor IX generates Factor IX A, an active serine protease composed of a 145 amino acid light chain and a 236 amino acid catalytic heavy chain, linked through disulfide bonds. Genetic alterations at the Factor IX locus such as point mutations, insertions and deletions, can lead to hemophilia B, also known as Christmas disease. Product :
1 mg/ml in Phosphate buffered saline (PBS) with 0.05% sodium azide, approx. pH 7.3. Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
Factor IX HC (V227) polyclonal antibody detects endogenous levels of Factor IX HC protein. Immunogen :
Synthetic peptide, corresponding to amino acids 227-291 of Human Factor IX HC. Conjugate :
Unconjugated Modification :
Unmodification

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  Western blot (WB) analysis of Factor IX HC (V227) polyclonal antibody at 1:500 dilution Lane1:H9C2 whole cell lysate(40ug) Lane2:Jurkat whole cell lysate(40ug) Lane3:K562 whole cell lysate(40ug) Lane4:AML-12 whole cell lysate(40ug)

Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

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Price/Size :

USD 368/1mg/vial

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Tips: 

For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).

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Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

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Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

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Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 

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Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.

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